fragment of a novel 中文意思是什麼

fragment of a novel 解釋
小說的片斷
  • fragment : n. 1. 碎屑，碎片，破片，斷片。2. 未完稿，斷簡殘篇。vi. ,vt. （使）成碎片，（使）分裂。
  • of : OF ＝Old French 古法語。
  • a : an 用在以母音音素開始的詞前〉 indefinite art 1 〈普通可數名詞第一次提到時，冠以不定冠詞主要表示類...
  • novel : adj. 新的，新穎的；新奇的，珍奇的，異常的。 a novel experience 新的經驗。n. 1. （長篇）小說。2. 〈N-〉〈常 pl. 〉 【羅馬法】新法，附律。
  1. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  2. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  3. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  4. Histochemical gus assay showed that the gus staining was observed only in the mature pollen and germination pollen tube. there is no detectable gus activity in other floral organs, leaves and stems. these results suggested that st901 is a novel pollen - specific gene and the 288bp promoter fragment ( - 297 ? xfrom the translation start codon atg ) is sufficient for pollen - specific expression and os - element regulatory of st901 promoter was possibly concentrated in the region - 297 to - 9

    通過對gus酶活性的組織化學定位分析，表明， st901基因啟動子驅動gus基因特異地在成熟花粉和花粉管中表達， st901基因具有花粉表達特性；且288bp （ - 297至- 9 ） （ atg定為+ 1 ）的啟動子區段足以驅動gus基因在花粉中的特異表達， st901基因啟動子的花粉順式表達元件可能位於- 297至- 9之間。
  5. However, there are some problems in the original method, such as low availability of the extracted fragments, error position of the match results, and high complexity of the algorithm etc. in this thesis, a novel method to extract fragments is proposed, and it improves the availability of the extracted fragments ; a novel match method called her is also proposed, which is a hybrid one combining the object ’ s edge and region features, and its advancement is showed in the experimental results ; a prototypal top - down image object segmentation system named io - seg is devised and implemented in this thesis, which is based on class - specific fragment and integrates the methods proposed in this thesis

    由於csf - seg方法存在提取的子圖可用性差、匹配位置誤差大、計算復雜性高等問題，通過深入分析和研究，本文提出了一種新的提取子圖的方法，提高了產生的子圖的有效性;在深入研究匹配方法的基礎上，提出了一種新的結合對象邊緣和區域的形狀匹配方法? ? her方法，通過實驗證明了該方法的優越性;最後，結合文中提出的方法設計並實現了一個top - down的圖像對象分割原型系統io - seg 。
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