full-length cdna 中文意思是什麼

full-length cdna 解釋
全長cdna
  • full : adj 1 充滿的，裝滿的。2 充分的，豐富的；擠滿的；（吃、喝等）盡興的，3 （精神）飽滿的。4 完全的，...
  • length : n. 1. 長，長度，長短。2. （時間的）長短，期間。3. （賽艇的）一艇的長度；一馬的長度。4. 程度，范圍。5. 【板球】球程；投至適當距離的球。6. 【語言學】音長。7. 一段，一節。
  1. Ah above showed that the full length cdna ej175 encoded pollen allergen - like protein and could be induced by abiotic stress factors

    綜合分析，推測該基因屬于病程關聯蛋白betvi家族。
  2. The two cdna fragments, ap - 2a full length cdna and ap - 2 a cdna fragments were inserted in frame into gst gene fusion system. with an ap - 2a monoclonal antibody we detected the expression of gst - ap - 2a

    將這兩段篩選所得cdna片段和ap - 2 cdna全長及ap - 2 cdna的分段形式用gst基因融合系統進行表達，並用ap - 2的單克隆抗體檢測了gst - ap - 2融合蛋白表達的情況。
  3. Mao et al ( 2002 ) cloned and identified the full - length cdna for ecbp21

    隨后，對ecbp21cdna進行了克隆及鑒定（毛國紅等， 2002 ） 。
  4. The full - length cdna of hbrp is amplified by pcr reaction and sequenced after cloning. 3

    在序列的兩端設計引物， pcr擴增全長cdna ，克隆后測序分析。
  5. Linearized full - length cdna was used as template then genomic rna of csfv was in vitro transcriped by t7 rna polymerase

    以線性化的全長cdna為模板，體外轉錄得到了csfv基因組rna 。
  6. The full - length cdna of ttom1, 1280 bp in length, encodes a deduced 288 a. a. protein with 7 - pass explasmic domains

    Ttom1全長cdna有1280個堿基對，編碼288個氨基酸，有7個跨膜區，是個典型的跨膜蛋白。
  7. Successful construction of infectious full - length cdna of csfv c - strain has provided an excellent tool for further study csfv c - strain on level of molecular biology

    這一技術為眾多病毒分子生物學研究領域提供了極為有價值的研究工具。
  8. Artificially using t - a cloning techniques and initiated one - step ligation of multi - fragments method were the main factors in the process of full - length cdna construction

    T - a克隆的巧妙運用和多片段一步連接法的成功運用是全長cdna得以快速構建的主要原因。
  9. Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system

    進一步通過rt . pcr克隆了ked樣蛋白編碼區全長cdna ，通過雙雜交驗證了全長ked樣蛋白與acamz的相互作用，結果為陽性。
  10. The full - length cdna sequence was finally generated by 5 ' race and 3 ' race respectively. at the same time, structure and function of ejol gene were primarily analyzed by bioinformatics method

    結果表明獲得的ejo3基因的cdna長度為1514hp ，開放閱讀框長度為1368hp ，編碼456個氨基酸。
  11. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  12. The full length cdna has 1677 bp and encodes a polypeptide of 329 amino acids

    小鼠pdip1基因cdna全長為1677bp ，編碼329個氨基酸殘基。
  13. Detailly, it was ( l ) to isolate and construct high efficiency expression vector of rice starch branching enzyme gene sbe2b and ( 2 ) to establish a high - effecient rice transformation system. total rna was extracted from maize endosperm 15dap ( days after pollination ). and cdna of sbe2b was obtained through rt - pcr. sequencing result showed that the full length cdna was 2. 4kbp, coding for 800 amino acids, with estimated mw 93kd

    本研究從授粉15天左右的玉米胚乳中提取了總rna ，採用rt - pcr方法，克隆了玉米胚乳的澱粉分支酶基因sbe2b ，測序結果表明，玉米胚乳sbe2b的cdna全長為2 . 4kb ，編碼800個氨基酸，推測的氨基酸的分子量為93000d ，該序列與genbank中登錄號為af072725的玉米sbe2b的cdna序列有98的同源性，與水稻、大麥、小麥等禾本科植物的有關澱粉分支酶的dna序列都有極高的同源性。
  14. Clone human wnk4 full length cdna into pgem - t vector human kidney total una was extracted and used as template to amplify wnk4 full length cdna with the forward primer ( 7 - 27 ) and reverse primer ( 3808 - 3833 ) with the long template expanded pcr system kit

    5 .原位雜交以小鼠腎臟總翩a為模板，擴增wnki基因外顯子1 ， 24一28 ，以a片段和wnk4基因外顯子13一16片段，純化后連接在pgem一t載體上。
  15. Phytoene desaturase ( pds ) has recently been identified as an important enzyme in carotenoid metabolitic pathway. a new full - length cdna clone encoding phytoene desaturase gene was isolated from stigma of saffron using rt - pcr and race techniques ( ( genbank accession no. ay 183118 ) )

    其中主要的活性成分是西紅花甙，需經類胡蘿卜素代謝途徑合成，而八氫番茄紅素脫氫酶（ phytoenedesaturase ）是此途徑中的一個早期關鍵酶。
  16. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail

    3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。
  17. We cloned full - length cdna of ca coding region via pcr amplification, but a negative result was obtained when the interaction of full - length ca and ga was tested in yeast two - hybrid system

    通過rt - pcr克隆了ca編碼區全長cdna ，但是在雙雜交系統檢測全長ca與g蛋白的相互作用時，結果為陰性。
  18. In our studies, we have used the nuclear localization signal department of medical genetics and development biology 4 ( nls ) selection system to isolate a novel gene from a mouse embryonic cdna library. the full - length cdna is about 1802bp and contains an open reading frame of 1296 nucleotides. it encodes 431 amino acid

    本研究工作，利用本室構建的核定位信號篩選系統從小鼠胚胎cdna文庫中克隆到一個新的全長cdna片段，分析表明在其1802bp育回軍區大學刃士學位論文的序列中含有一個長1293hp的開放閱讀框，編碼431個氨基酸。
  19. Successfully cloned and constructed infectious full - length cdna of attenuated lapinized csfv chinese - strain ( derived from spleen ) could make us get pure rna virus genome of csfv c - strain, and further study and utilize mutation, deletion, insertion and substitution of csfv gene on dna molecular level

    中國豬瘟兔化弱毒（脾淋毒）全長感染性cdna的克隆和構建，可以使我們得到純粹的csfvc -株rna病毒基因組，在dna水平上研究和利用csfv的基因突變、缺失、插入和替換。
  20. In this study, by combining rt - pcr and race, a full - length cdna encoding the lagurus lagurus zp3 ( lzp3 ) was isolated from the lagurus ' s ovary and cloned into a prokaryotic expression vector and a eukaryotic expression vector for further immunocontraceptive investigations

    本論文研究工作採用rt - pcr與race相結合的方法，首次克隆了草原兔尾鼠zp3全長cdna並進行了序列分析，構建了真核表達質粒，為草原兔尾鼠免疫不育的研究奠定了基礎。
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