fusion gene 中文意思是什麼

fusion gene 解釋
融合基因
  • fusion : n. 1. 熔解，熔化；【物理學】（核）聚變，合成。2. 〈美國〉融合；（政黨等的）合併，聯合。
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  2. Therefore, toreniafournieri is a very suitable model plant for studies of double fertilization in living material. we transferred the fusion gene ofgfp : mtn to toreniafournieri by leaf disc transformation mediated by agrobacterium and gain transgenic plant

    我們將嵌合基因gfp : mtn通過葉盤法導入藍豬耳，探索了藍豬耳遺傳轉化的方法和轉基因苗的再生條件，建立了成熟的藍豬耳轉化系統。
  3. Construction of p21 egfp fusion gene expression vector and its expression in hrpe cells

    15細胞乙型肝炎病毒復制的實驗研究
  4. Construction and characterization of stable 1 - integrin - gfp fusion gene overexpressing hcc cell lines

    融合蛋白穩定過表達肝癌細胞系的建立
  5. By genetic engineering methods, ureb gene and ureb - hspa fusion gene were amplified by pcr and cloned into a prokaryotic expression plasmid ptrc99a - asd, and identified recombinant plasmid was then

    口服、鼻飼免疫balb / c小鼠，在腸液和血清中可以分別檢測到針對hpylori的特異性分泌型iga和址g抗體。
  6. To study the localization of the peptide, we also generated recombinant viruses containing the ctl - egfp fusion gene, following observation of infected larvae by such viruses will provide insight into the function of baculoviral conotoxinlike genes

    為了研究毒素在蟲體中的定位，構建了類蝸牛毒素和增強的綠熒光蛋白融合的重組病毒racbacctlgfp2和rhabacctlgfp ，為下一步研究奠定了基礎。
  7. Based on the foundation research of the interaction of virus and host actin, we recombine gfp gene - a reporter gene - with 5c actin gene of drosophila melanogaster, a gfp - actin fusion gene was obtained

    本文在總結前人關于病毒與宿主肌動蛋白相互作用的研究的基礎上，利用綠色熒光蛋白基因為標記基因，與果蠅肌動蛋白基因5cactin基因融合，構成gfp - actin融合基因。
  8. Construction and expression of recombinant eukaryotic expression plasmid of ubiquitin and epstein - barr virus nuclear antigen 1 fusion gene

    病毒核抗原1的融合表達載體的構建及表達
  9. Gfp expression was monitored using a fluorescence microscope. the result showed that the fusion gene was expressed at a low level

    利用脂質體轉染的方法在美國棉鈴蟲細胞hz中表達了gfp - actin融合基因。
  10. Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr

    從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。
  11. To solve this problem, we used bac - to - bac system to recombine gfp - actin fusion gene and gfp gene under the control of the promoter of polyhedrin gene respectively. two recombinant virus : acmnpv - gfp - actin which contains gfp - actin fusion gene and acmnpv - gfp which contains gfp gene were obtained. the latter was set as a control

    為了解決以上問題，在以上實驗的基礎上，我們利用bac - to - bac系統分別將gfp基因和gfp - actin融合基因重組于多角體基因啟動子之下，構建了兩種重組病毒，即含gfp - actin融合基因的重組病毒acmnpv - gfp - actin和含gfp基因的重組病毒acmnpv - gfp 。
  12. In this study, vp7 gene and ctb - vp7 fusion gene expression vectors were constructed, and a high - efficient genetic transfomation system of carrot ( daucus carota l. ) was established. vp7 gene was amplified from cdna of rotavirus antigene by pcr and was inserted into expression vector pbi121 containing no gus gene

    本實驗以輪狀病毒的vp7為目的基因，構建了vp7基因的表達載體和vp7 - ctb融合表達載體；以胡蘿卜為受體材料，建立並優化了胡蘿卜的轉化體系，獲得了能正常轉錄vp7基因的轉基因胡蘿卜植株。
  13. The result demonstrated that the ctbvpl fusion gene was inserted into the c. reinhardtii chloroplast genome and after three rounds of spc selection the recombinant chloroplast dnas were the majority, and the wild - type chlorop last dnas

    W七sternblot和elisa免疫雜交分析表明ctbvpi融合蛋白在衣藻葉綠體中得到表達，表達量占可溶性總蛋白量的3一4 % （ lmg可溶性總蛋白含有30一40pg的重組蛋白） 。
  14. Construction and expression of recombinant human thymosin fusion gene in pichia pastoris

    在畢赤酵母中的表達
  15. Cloning and expression of fusion gene of transmembrane domain of human cd20 and g3pn in escherichia coli

    端融合基因的克隆及其在大腸桿菌中的表達
  16. Put this fusion gene under the control of the promoter of ie1 gene, and then an eucaryotic cell expression plasmid vector pgem / ie1 " - gfp - actin resulted

    將該融合基因置於ie1基因啟動子的控制之下，構建成了真核表達質粒pgem ie1 - gfp - actin 。
  17. On the base of construction of pbi121vp7, we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7

    設計具有ndei和saci單限制性酶切位點的vp7基因引物，通過pcr擴增出vp7基因並經測序驗證。
  18. Recombinant plasmids pgfpexpa that containing cry3apro - gfp fusion gene and pgfpexpb that containing btl - bthpro - gfp fusion gene were transformed into e. coli and b. thuringiensis plasmidless strains, respectively

    該質粒的dna序列已被genbank不11genome收錄，序列號分別為af202532不11nc001272 。
  19. The fusion gene ofgfp : mtn ( mtn is binding domain of microfilament binding protein talin, which can show the microfilament in living cell ) was transferred to arabidopsis thaliana. stable expression of gfp - mtn can visualize the actin cytoskeleton in different types in living cells without affecting cell morphology and function

    將gfp : mtn （ mtn是微絲結合蛋白talin的微絲結合域，可以顯示活體細胞中微絲的結構）導入擬南芥后，發現表達的融合蛋白能夠標記微絲骨架，同時又不影響植物細胞的正常形態和功能。
  20. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
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