fusion yeast 中文意思是什麼
fusion yeast
解釋
分裂酵母-
The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain
成膜素相關蛋白1 （ phrip1 ）是一個含608個氨基酸的蛋白質，它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制，對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中，根據phrip1的序列設計引物對其進行pcr擴增，得到該基因后將其連接到了pks質粒上，並進一步構建成了誘餌質粒peg202 - phrip1 。 -
By yeast two - hybrid assay, aes was found to interact with gp130 intracellular region through its conserved q domain. results from the yeast two - hybrid assay, gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain, and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction. to investigate the consequence of this interaction, tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector
在通過酵母雙雜交分析確定aes通過q結構域與sp130分子胞漿區結合的基礎上，為確定tle1分子是否也能通過保守的q結構域與gp130分子胞漿區結合，我們通過pcr擴增編碼gp130胞漿區與tleq分子不同結構域的cdna ，構建了含有這些不同結構域的酵母雙雜交載體，通過酵母雙雜交分析證實： tle1分子通過其氨基端的q結構域與gp130分子胞漿區近膜段結合。 -
The yeast recombinant expression vector pplc3. 5k - ndrg2 was constructed, then the ndrg2 protein was expressed in soluable form in pichia. after purification by metal chelate affinity chromatography, we get 6his - ndrg2 fusion which be nature in structure and will be useful to reseach its functions in future
為研究ndrgz的結構與功能，構建了ndrgz的酵母融合表達質粒，並在比赤酵母中得到可溶形式的表達；利用金屬螫合親合層析純化出了6his融合的ndrgz蛋白。
分享友人