gal e 中文意思是什麼
gal e
解釋
epimerase]半乳糖表異構酶-
Aries still remember when year begging and receiving interview by e - fesco magazine aries told readers about new year wishes, including love aspects and career aspect, he almost fulfilled career one but love aspects is a little bit different and didn ' t “ hold her hands and watch the red leaf in autumn together ” but the god of love didn ' t forget aries in 2007, and give him opportunity to meet excellent gal, but somehow aries is too young and didn ' t cherish well, after a painful twisting and self - blame, aries decide to achieve love wishes in 2008
在年初接受《外服時代》雜志采訪時，永潔表達了事業和愛情兩方面的新年願望，其中事業部分基本達成，愛情部分少有出入，並沒有牽著她的手一起欣賞秋天的楓葉，事實上，愛情之神在2007年並未將永潔遺忘，生命中也遇到清清楊婉一般的女子，怎奈永潔同學年少氣盛，不懂得珍惜，錯失良緣，在一番痛定思痛，沒有一番寒徹骨哪得梅花撲鼻香的自我檢討與譴責后，永潔同學下定決心在2008年達成心愿。 -
Then the linked products were transformed into the high competent cell of e. coli dh5a. based on - complementation of the detective - galactosidase, positive recombinant clone were screened from x - gal plate
從引物和基因序列的比對分析結果看， zhyf006序列與上下游引物的配對比例分別為s4 -
A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
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