gal virus 中文意思是什麼
gal virus
解釋
家禽病毒-
Fixed with gluteraldehyde, stained with ph 7. 4 phosphate buffered x - gal dye solution, observed by reverse microscope, the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus
病毒感染后的8h , 12h , 16h , 20h提取中腸組織,按常規石蠟包埋切片進行洗滌、透明、浸蠟、包埋、切片、粘片等操作程序,製成連續切片,在倒置顯微鏡下逐一進行觀察計數。 -
After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞,採用甲基纖維素固定病變, x - gal染色,經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株,命名為rprv - lacz 。
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