galactosidase 中文意思是什麼

galactosidase 解釋
半乳糖激酶
  1. In this paper, thirty strains of yeast were isolated from nineteen samples of fermented milk ( ten koumiss samples, three fermented goat milk samples, four fermented milk samples, two shubat samples ) which were collected from the west - centre region of inner mongolia from which we aimed to select the yeast with high vitalistic ? galactosidase. according to the results of dedermination that were the metabolism rate and ratio vitality of ? galactosidase, stk - 1 - 1 has been selected. the result of identification showed that stk - 1 - 1 belonged to kluyveromyces marxianus of kluyveromyces

    本研究主要以內蒙古中西部地區牧區採集的19個發酵乳樣品（ 10份酸馬奶、 3份發酵山羊奶、 4份酸牛奶和2份發酵駝奶）為研究對象，分離獲得30株酵母菌，從中篩選乳糖酶高活力酵母菌株。根據乳糖代謝率和乳糖酶比活力的測定結果，選出菌株stk - 1 - 1為優勝菌株。經鑒定，該菌株屬克魯維酵母屬（ kluyveromyces ）的馬克斯克魯維酵母（ kluyveromycesmarxianus ） 。
  2. Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned

    乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。
  3. Cloning and sequence of - galactosidase gene of lactobacillus burgarlus

    半乳糖苷酶基因克隆和序列分析
  4. Ability to a - complement of the ea / ed protein was determined by the addition of onpg western blot test with rabbit to e. coli p - galactosidase pcab as first antibody was used to verify the fusion proteins

    以兔抗p半乳糖昔酶抗體做western blot以證實與gst融合表達的ea工d蛋白是p半乳糖昔酶的成分。實驗結果1
  5. Cloning and expression of - d - galactosidase from coffee bean coffea liberica amp; amp; coffea canephora

    半乳糖苷酶基因的克隆與表達
  6. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  7. The effect of - galactosidase and polygalacturonase on peach ripening and softening

    半乳糖苷酶及多聚半乳糖醛酸酶對桃果實成熟軟化的影響
  8. We succeeded in constructing the fusion protein plasmids of ea and ed of p - galactosidase. 2

    構建的質粒能高效表達具有較高a一互補活性的gs …蛋白、 gsthd蛋白。
  9. The a - complementation reaction of & - galactosidase ea and ed was also used in dna cloning, protein protein interactions monitoring and expressing immunoassays studying

    -半乳糖苷酶的ea 、 ed這種-互補性還被用於分子生物學、蛋白質相互作用的監控、表達免疫分析等方面的研究。
  10. Then the linked products were transformed into the high competent cell of e. coli dh5a. based on - complementation of the detective - galactosidase, positive recombinant clone were screened from x - gal plate

    從引物和基因序列的比對分析結果看， zhyf006序列與上下游引物的配對比例分別為s4
  11. P - galactosidase of e. coli which is scarce in human blood plasma and has a variety of substrates, is one of the most frequently used enzyme labels. it is used both in heterogeneous and homogeneous enzyme immunoassay

    大腸桿菌的-半乳糖苷酶，因人血漿中缺乏此酶，並具有大量易得的底物，被用於均相及非均相免疫分析，是目前最常用的標記酶之一。
  12. Recombinant fpvs were selected and purified by blue plaques expressing 3 - galactosidase. the expression of foreign genes in rfpvs were confirmed by ifa. trails for evaluating protective efficacy of rfpvs against very virulent mdv or aiv challenge

    純化后脂質體轉染，藍斑篩選純化得到穩定的重組病毒rfpv - ps - ha - pe / l - f ，間接免疫熒光法證實， ha基因和f基因同時得到了表達。
  13. Analysis of activity of p - galactosidase showed that cryld - lacz expressed differently in the same host because of different carbon sources and differenly in different hosts utlizing same carbon sources, suggesting that metabolism pathway of carbon source in hd - 133 - 5 was special

    首次報道了cry1d - lacz在同一菌株中的表達因碳源不同而異，在碳源相同的條件下不同菌株的表達也不相同，而衍生菌株hd - 133 - 5的代謝途徑非常特殊。
  14. No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract

    將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。
  15. Light microtechnique and sa - galactosidase method was used to study the effects of super - high - concentration of glucose on the senescence of human diploid fibroblast 2bs cells, ros and the membrane potential of mitochondria during this process were measured. our results showed that 200 mmol l of glucose inhibited the growth of 2bs cells, led to the changes of reactive oxygen species and decrease of mitochondrial membrane potential, and caused senescence of 2bs cells rapidly. it supports the hypothesis of oxidative damage of senescence. moreover it is a better system for the study of the effects of ros during the process of replicative senescence

    利用光學顯微鏡觀察和酸性-半乳糖苷酶染色技術研究了高濃度葡萄糖對人二倍體成纖維細胞2bs細胞衰老進程的影響，並用流式細胞儀檢測了此過程中活性氧和線粒體膜電位差的變化。結果表明： 200 mmol l的葡萄糖對2bs細胞有生長抑制作用，能引起活性氧含量的變化，導致線粒體膜電位差顯著下降，並誘導了細胞的衰老。這為氧化損傷假說提供了新的證據，並為研究活性氧和復制衰老之間的關系提供了較好的體系。
  16. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  17. Primary cultured nscs ( labbled by hoechst33342 ) and c17. 2 ( a kind of immortal nsc cell line expressing b - galactosidase ) were injected into the lateral ventricles of neonatal mouse, then the cells " survial, integration and migration were compared 1w and 6w after the transplantation. the results are as follows : after lw, the living cells of primary nscs were much more than c17. 2 ( p < 0. 01 ), besides, the former mainly resided in the ventricle whereas the latter integrated to the parenchyma

    利用此條件，在體比較了原代nscs及永塵化神經幹細胞系c17 . 2相同條件下移植后的異同，即將兩種細胞注射于新生小鼠側腦室，分別於1w ， 6w后觀察比較它們的存活、整合及遷移情況，結果如下： 1w時，原代nscs存活的細胞數遠大於c17 . 2 （ p 0 . 01 ） ，且主要分佈於腦室系統內，而c17
  18. These results suggest that kyot plays an important role in the development of testis and spermatogenesis. 2 we performed yeast two - hybrid screening of a cdna library from human lymph nodes using kyot2 as a bait protein. 42 clones were gotten after 5xl06 were screened by four kinds of nutrition limitation and p - galactosidase assay, 22 clones were got after restriction of positive clones, at last, 13 genes were got by sequence assay including rbp - j known as the interacting protein with kyot before and two novel genes

    對此22個克隆進行序列分析，共獲得13種基因，其中包括已知的kyot相互作用蛋白rbpj和兩個未知基因，也包括在哺乳動物睪丸中特異3第四軍醫大學博士學位論文性表達的蛋白piasi ，同時還篩到了具有可與lm結構鞠互作用的pdzdomain分子緊密連接蛋白2和兩種同屬于kg家族的蛋白ringi和polycomb2
  19. A novel homogeneous immunoassay system, cloned enzyme donor immunoassay system ( cedia ), has been developed by henderson in 1986 on the basis of a - complementation reaction of 3 - galactosidase ( e. coli ). in the cedia test, genetic engineering was used for the generation and selection of enzyme acceptor ( ea ) and enzyme donor ( ed ) of p - galactosidase

    Henderson等人在1986年利用基因工程技術生產大腸桿菌-半乳糖苷酶酶受體（ ea ）和酶供體（ ed ） ，並利用其-互補功能創立了克隆酶供體免疫分析（ cedia ）技術。
  20. Objective to prepare ea and ed protein of p - galactosidase with gst fusion protein by pgex - 4t - 2 expression system, and study its a complementation activity. to lay a good foundation for further study of cedia and utilization in expression immunoassay. methods an artificial synthesized dna segment coding for residues 6 - 56 ( modified ) of p - galactosidase ( ed protein ) was ligated to pgex - 4t - 2 vector

    實驗方法通過人工合成編碼d半乳糖昔酶ed蛋白的dna並插入原核表達載體pgex 4t 2 ；從陀v p galactosldasecontrolvector質粒中用sal及earl進行雙酶切得到編碼卜半乳糖昔酶a一受體（ ea ）的大部分dna ， n端再加上約60hp人工合成洲a ，連接后轉入pgex葉t億載體中。
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