gel column 中文意思是什麼

gel column 解釋
凝膠柱
  • gel : n. 凍膠，凝膠（體）；定型發膠。vi. （-ll-） 成凍膠，膠化。
  • column : n 1 【建築】圓柱；圓柱狀物〈如煙柱〉。2 【軍事】縱隊 （opp line）; 隊；（艦隊的）縱陣，縱列，艦列...
  1. We got 4 parts from the extraction of astragalus variabilis bunge through systemic research ways of phytochemistry, which respectively are the part of petroleum ether, the part of petroleum ether, the part of etoa and the part of n - buoh the part of n - buoh are isolated by the successively methods of silica - gel column chromatography, tlc and sublimation

    摘要採用植物化學成分系統研究的方法，從變異黃芪醇提總浸膏中分段萃取得到石油醚提取部分、氯仿提取部分、乙酸乙酯提取部分和正丁醇提取部分。
  2. The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent. after concentrating and drying, relatively pure fr - 008 was obtained. hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008, candicidin and a mixture of both

    對鏈黴菌fr - 008和灰色鏈黴菌imru3570的rflp分析發現，它們的染色體具有很高同源性； pfge的比較研究發現，這兩個菌株的明顯區別是鏈黴菌fr - 008攜帶有兩個線性質粒，而在灰色鏈黴菌imru3570中則沒有這兩個線性質粒。
  3. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  4. Methylbenzyl amine as chiral auxiliaries to react with racimic ketorolac. then authors got optical amides. the diastereoisomeric mixture were separated by column - chroatography on silica gel. the results of separation depend on the structure of r

    苯乙胺作為手性輔基，與外消旋酮咯酸生成一對光學活性的酰胺衍生物，再用簡單易行的柱層析方法分離其非對映異構體。
  5. This material was loaded onto a silica gel column and the column was eluted with chloroform several times until the fraction of interest was collected. finally we obtained some light yellow green ointment liquid f i

    採用硅膠作干層析柱填充物，用氯仿作展開劑和洗脫液，反復硅膠層析，對柄海鞘鞘囊氯仿浸提物進行分離、提取與純化，最後得到一淺黃綠色的油狀液體fraction （以下均稱f ） 。
  6. A white needle crystal was isolated and purified on systematic extraction of the alkaloid by silica gel column and gradient sublimation from oxytropis glabra

    摘要採用生物?系統提取、矽膠柱層析分離及梯度升華技術從小花棘豆乾草粉中分離出一種白色針狀結晶。
  7. The methods such as distn. and silica gel column chromatography were used to sep. the various components of the thickener, and the ir spectrometry, gc - ms combination and esi - ms were used to certify the structures of the different components

    用蒸餾和矽膠柱色譜等方法對增稠劑中的各組分進行分離，並用紅外光譜法、氣質聯用和電噴霧質譜法對各組分進行結構鑒定。
  8. An active metabolite was obtained by purification with precipitated by ethanol, sephadex g - 25 gel, deae - cellulose ion exchange resin and silica gel column chromatography

    經乙醇（ 95 ）沉澱、 sephadexg - 25凝膠層析、 deae -纖維素離子交換層析和硅膠層析純化，得到抑制黑麴黴生長的單一組分。
  9. Conclusion the co - utilization of silica gel column and gel chromatography can greatly improve the efficiency of isolation and purification

    結論硅膠柱色譜和凝膠柱色譜的混合利用可以提高供試品的分離純化效果。
  10. Chromatographic column : the most commonly used filler of chromatographic column is chemically bonded silica gel

    色譜柱最常用的色譜柱填充劑為化學鍵合硅膠。
  11. During the course of purification, the method of orthophenanthroline staining is used to detect the ferritin. 30 | il of the fractions eluted from the aca ^ gel column were pulled into the cells of the minim incubation plate. then sopil 20 % dithionite and 40 | il 0. 1 % orthophenanthroline solution were pulled into respectively

    在酶標板小孔中加入30 l待測液，加入30 l20的連二亞硫酸鈉溶液還原后，再加入40 l0 . 1鄰菲咯啉溶液，根據有無桔紅色顏色產生確定是否含鐵蛋白。
  12. Methods the optimal elution requirement was found by comparing several different elution agents, and the pure compound was purified by silica gel column and gel chromatography

    方法通過最佳展開劑的選擇，找到硅膠柱色譜系統的最佳洗脫條件，粗分離后的供試品通過凝膠柱色譜系統分離純化得到純供試品。
  13. The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively

    我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析，並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物，從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子，其在sds - page電泳中的分子量約為98 . 5kda 。
  14. 3. the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100. the specific activity of the purified enzyme was increased by 37. 9 folds over crude extract, and the recovery yield was 8. 9 %

    以粗酶液為起始，經硫酸銨分級沉澱、 sephadexg - 100凝膠過濾后，分離純化了該酶，純化倍數約為37 . 9 ，酶活力回收率8 . 9 。
  15. The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod

    通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。
  16. 4m phosphate buffer ; the second is that the crude enzyme runs on sephadex g100 - g50 column under the same experimental conditions ( only with sephadex g100, g50 ). at last polyacrylamide gel electrophoresis and sod assay tell us both of products are highly purified sod

    因而不管從第一次的純化來看，還是從文獻上報道的來看，本實驗的串聯柱層析是較為合理有效的一種生產方法，值得推廣。
  17. In the process of experiment, we separated better efficient antioxidative fractions by silica gel column chromatography with different extensible reagent, ethyl acetate : hexane ( 3 : 7 ) and chloroform : methanol ( 9 : 1 )

    實驗中採用乙酸乙酯環己烷（ 3 : 7 ） 、氯仿甲醇（ 9 : 1 ）為展開劑依次二次干柱層析分離出抗氧化物質。
  18. Sal - gel column is a new - type of chromatographic column with high temperatures tolerance, excellent separation and repeatability

    摘要溶膠凝膠柱是一種新型色譜柱，具有耐高溫、分離效果佳、重現性好、製作工藝簡單等特點。
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