gene base 中文意思是什麼

gene base 解釋
基因庫
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • base : n 1 基底，基，根基，底座；底層，底子；（紀念碑等的）基址；（山）麓。2 【軍事】基地，根據地。3 根...
  1. The gene-base for adaptability must be increased.

    適應性的基因基性必然會有所增加。
  2. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  3. Some tendency of tn5gusa5 transposition were found that all preferred sites of tn5gusa5 in xcc 8004 genomic dna are in at - rich regions ; target sequences of tn5gusa5 have some features that the probabilities of guanine and cytosine are high respectively at the head and tail base of target sequence ; the level of gene transcription does not influence insertion density of tn5gusa5 significantly

    結果表明， tn5gusa5插入位點有一定的規律性： tn5gusa5在xcc8004基因組上傾向于插入低gc含量（ 50左右的區域插入密度最高）區段；插入位點的靶序列有一定的特異性，在靶序列的首位鳥嘌呤出現的幾率高，而在靶序列的末位胞嘧啶出現幾率高； tn5gusa5的插入密度與該區段基因的轉錄水平無明顯關系。
  4. Abstract : hill mourning the special and advantageous natural conditions, ailaoshan become enormous natural biological species of germplasm resources gene pool, yunnan as " animals and plants " kingdom of " natural museum " and " specimen garden, " is a variety of integrated science experiments, is the ideal many disciplines scientific investigation and research base, is the scientific expedition, adventure, photography, research and returned to the natural forest of choice for this paper, through the ailaoshan reptilia from the investigation, ailaoshan the right reptilia is a more systematic and well described

    摘要：哀勞山特殊而優越的自然條件，使哀牢山成為巨大的天然生物種的種質資源的基因庫，成為雲南"動、植物"王國中的"天然博物館"和"標本園" ，是多種自然科學的綜合實驗地，是眾多學科的理想的科學考察和研究基地，是開展科考、探險、攝影、科研、回歸自然、森林的首選之地，本文通過對哀牢山爬行綱的抽取調查，對哀牢山的爬行綱給出一個較系統的，完善的敘述。
  5. The other amylase activity is 40 times of native amylase. the non - amylase activity gene has 9 base changes, resulting in 5 amino acid changes from the native enzyme

    其中，無酶功能基因突變位點最多，導致全長編碼區內5個氨基酸突變，低酶活和高酶活基因各兩個突變位點。
  6. The mature region cdna of melittin of apis cerana was cloned by using puct - vector developed from pucis vector. the sequence was compared with the corresponding region of melittin of apis mellifera reported in genbank, the mutant site was found in 33rd base ( a : apis mellifera ; g : apis cerana ), but the primary structure of the two proteins were identical, which suggested melittin, as an important defensive gene, was very conservative in evolution

    測序結果顯示與意蜂（ apismellifera ） melittin成熟區序列相比，中蜂（ apiscerana ） melittin成熟區序列在第33位存在差異（意蜂為a ，中蜂為g ） ，但兩者蛋白質一級結構相同，說明melittin作為蜜蜂一種重要的防禦基因在進化上保守。
  7. It is possible that exogenous dna fragment integerated into acceptor genome, changing gene base sequence or base deletion or base insertion, inducing to mutant at gene level

    這可能是外源dna片段整合進受體的基因組中，改變基因的順序或者引起基因堿基的缺失、插入，從而在基因水平上發生突變。
  8. With the base of related research on nucleic acid immunization, the technology was used to develop th e research and application of cpti transgenic plant. a new antibody preparation method by nucleic acid immunization to assay the expression of the transgenic gene was explored and compared with the traditional one which immune animal by protein

    在國內外大量核酸免疫的研究基礎上，本研究首次將核酸免疫技術應用於轉基因植物檢測研究中，探討一種核酸免疫法制備抗cpti抗體來檢測基因表達的方法，並與傳統的蛋白免疫方式制備抗體進行比較。
  9. Further sequence analysis show that only 6 base pairs of nucleotide and 2 amino acids are different between them. the homological cry3aa gene was expressed in escherichia coli. and the expressed products which contain a fused peptide of 66 - 97 kilo - dalton was observed by means of sds - page

    生物活性測定結果表明該菌株對榆藍葉甲（ pyrrhaltaaenescens （ fairmaire ） ）和光肩星天牛等鞘翅目昆蟲具有較高的毒力，因此初步確認該菌株屬于cry3類； （ 2 ）發現該菌株中編碼毒蛋白的基因位於質粒上，並且已經成功地克隆到該基因。
  10. In the third chapter, the experiments of the anaerobic continuous fermentation of glycerol by klebsiella are explained. on the base of the experiments, dynamic models for the reaction of the microorganism are builded. at the same time, a certain dynamic models based on enzyme catalyzing and gene controlling is listed

    第三章數學模型的建立，介紹肺炎桿菌將甘油發酵生產1 ， 3 ?丙二醇的實驗過程和在實驗的基礎上建立微生物反應動力學模型的過程，並進一步給出了幾個酶催化反應和基因調控動力學模型。
  11. A 40 - base polymorphic repeat sequence located in the 3 ' - untranslated region of the dat gene was purified and amplified by polymerase chain reaction ( pcr )

    將位於多巴胺運輸器基因3 '端未轉譯區段的40 -堿基多形性重復序列予以純化、經聚合酶鏈鎖反應放大。
  12. This thesis studied on bacillus thuringiensis strain bt886 which was separated and selected by researchers of our laboratory. according to the observation of crystal shape and the bioassay of motschulsky and fairmaire, bacillus thuringiensis strain bt886 was identified as cry3 type, and the conclusion was assured by the further study on molecular level. the 1956 base pairs full lengrh homological cry3aa gene which was toxic to motschulsky was cloned and sequenced

    以該菌株為材料，克隆出了對光肩星天牛（ anoplophralabripennis （ motsch . ） ）具有毒殺作用的cry3aa同源基因，並且對該基因進行了人工改造、人工合成、大腸桿菌表達、生物活性測定、雙元表達載體的構建以及對楊樹的轉化等一系列研究，主要結果如下： （ 1 ）顯微觀察該菌株所形成的伴孢晶體為方形。
  13. In e coli, dna polymerases are key enzymes involved in two distinguished pathways contributing to untargeted mutagenesis. replication of dna by pol v ( umuc ), in the presence of umud1, reca and single strand binding protein ( ssb ), is highly mutagenic and exhibits a predominant mutation pattern of base transversion. another error prone polymerase involved in untargeted mutagenesis is pol iv, encoded by dinb gene

    在umud ' ， reca和單鏈結合蛋白ssb的協助下， polv （ umuc ）能在單鏈模板上催化dna合成並產生高頻率的以堿基顛換為主要形式的突變；另一個與非定標性突變有關的易誤dna聚合酶是pol ，為dinb基因的編碼產物。
  14. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  15. In addition, base on the certain level of genetic differentiation of among the 4 possible natural populations, it is advised that adult plants or seedlings of ginkgo be transplanted mutually in order to enhance the gene flow among them and preserve the genetic diversity of ginkgo furthest

    因銀杏群體間已出現了一定程度的遺傳分化，建議在可能的自然群體間進行植株和種子幼苗相互移栽，以增強群體間的基因流，最大限度地保護銀杏的遺傳多樣性。
  16. A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4. 1 softwarre, they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain, therery, they serve mutually as template and primer, and their extension were carried out to synthesize 90 bp " gnrh / trs

    本研究根據genbank中已發表的人gnrh2基因mrna序列以及轉運肽（ transporter ， trs ）基因核苷酸序列，藉助oligo4 . 1設計了一對寡核苷酸引物，以引物3末端的短互補序列退火形成小段雙鏈，從而互為模板和引物，通過引導合成長達90bp的gnrh trs序列gnrh trs 。
  17. An optically addressed spatial light modulator ( oa - slm ) made of brd96n and based on its good nonlinear optical characteristics is presented. an experiment study on the mutual conversion of coherent - to - incoherent image is carried out, and the mutual modulation of two spatial light beams can be realized base on the gene - variant br films

    基於br膜良好的非線性光學特性，開展了br膜作為一種光尋址的空間光調制器（ br - oa - slm ）的研究，進行了相干光圖像信息與非相干光圖像信息的相互轉換實驗，實現了空間兩束光的相互調制。
  18. Sequence variation analysis exhibits high transition to transversion substitution ratios, and transitions and transversions are unsaturated in the sequence of cytochorme b gene. the base composition of the cytochrome b sequences was highly biased. using a structural model of the cytochrome b protein as a template, we divided cyt b sequence data for species within anserifomes into three functional domains

    3 ）序列具有較高的轉換顛換比，基因序列中的轉換與顛換都未飽和，序列中的堿基組成高度偏倚： 4 ）按照細胞色素為蛋白結構模型，細胞色素b基因全序列可分為三個功能區，堿基替換飽和狀況與不同功能區相關聯。
  19. 9 expression of mxmybl gene in e. coli was experimented. the structure of its recombinant expression vectors had not base mutant and drift. sds - page of pet - mxmybl proteins showed that there was a strengthened protein band in expectant 38kda protein marker

    構建的原核表達載體結構正確，未出現堿基突變及移碼現象； lmmoffeiptg誘導zh后，在預期的蛋白分子量約38kda處出現一條表達加強的蛋白帶。
  20. The low - enzyme activity gene has 2 base changes, resulting in short amino acid sequence with native enzyme

    其中低酶活基因編碼區內一突變位點導致氨基酸序列的提前終止。
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