gene clone 中文意思是什麼

gene clone 解釋
基因克隆
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • clone : n. 1. 【生物學】純種細胞，無性系。2. 復製品；（幾乎）一模一樣的人。3. （不動腦筋）機械行事的人；機器人。vt. 把…培養為純種細胞；無性繁殖；〈比喻〉復制。
  1. The ast gene was located in bac clone t13m11

    初步確定該bac克隆中的基因t13m11 . 8可能是ast基因。
  2. However, only a few host factors with clear in vivo function have been identified. by using pcr and 5 " and 3 " race, we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene, tom1, which supports the replication of tobacco mosaic virus

    根據從擬南芥中已經克隆的支持煙草花葉病毒復制的基因tom1序列設計一對特異性引物，用rt - pcr的方法獲得番茄同源基因的部分序列，然後利用5 』 race與3 』 race方法從番茄中克隆出全長ttom1基因。
  3. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  4. The sequence analysis indicated that eight of the nine analogues contained the conserved motifs of nbs - lrr of disease resistant genes, such as p - loop ( kinase la ), kinase 2, kinase 3a and trans - membrane domain. the sequence of amino acid of clone cr271 was highly homologic ( 61 % identify and 76 % similarity ) to blight resistance gene xal of rice to xanthomonas oryzae pv

    然後進行了序列的測定，通過全序列分析，結果表明：所獲得的8個抗病基因同源序列均含有nbs - lrr類抗病基因的保守序列，如p - loop （ kinase1a ） 、 kinase2 、 kinase3a以及跨膜結構域等。
  5. Clone and expression of mycobacterium tuberculosis gene rv1009 in e. coli

    1009基因蛋白的克隆和表達
  6. We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression

    對其進行缺失突變，構建5個缺失表達載體轉基因擬南芥，葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達，說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。
  7. The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine

    利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ，並對其進行原核和真核表達，是建立小鵝瘟分子診斷方法、構建vp3基因重組禽痘病毒活載體疫苗的基礎，具有極為重要理論和實踐意義。
  8. Gene clone and sequence analysis of glycerol dehydratase

    甘油脫水酶結構基因
  9. To clone large and random dna fragments, the second generation of yac, pjs97 - pjs98 was modified as gene replacement vector in streptomyces

    4kb和1 . okb片段，而且還插入了勿，七刃j和spc心t廠抗性基因。
  10. An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined

    將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。
  11. It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his

    本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體（ pok8266 ）為模板，通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點，形成含有酶切位點（ nspv ）的突變體（ p1p4 ） 。
  12. Full - length clone of protein ht036 gene interacting with endostatin and its molecular mechanism in promoting the apoptosis of vascular endothelial cell

    036基因的全長克隆及其促血管上皮細胞凋亡的分子機制
  13. The objectives of this study are to clone the ban fragment of brassica napus and to apply the floral - dip in the transformation of oilseed rape to establish a convenient method for oilseed rape breeding. ban is dfr - like gene in chalcone synthesis pathway functioned as a negative regulator, and has been well studied in arabidopsis thaliana

    為利用轉基因技術創造甘藍型黃籽油菜，本文克隆了與甘藍型油菜（ brassicanapus ）色素生物合成有關的ban基因同源片段，並探索了甘藍型油菜花序浸泡法轉基因新方法。
  14. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  15. Phytoene desaturase ( pds ) has recently been identified as an important enzyme in carotenoid metabolitic pathway. a new full - length cdna clone encoding phytoene desaturase gene was isolated from stigma of saffron using rt - pcr and race techniques ( ( genbank accession no. ay 183118 ) )

    其中主要的活性成分是西紅花甙，需經類胡蘿卜素代謝途徑合成，而八氫番茄紅素脫氫酶（ phytoenedesaturase ）是此途徑中的一個早期關鍵酶。
  16. Gene cloning differential fragment gha27 was produced by cdna - aflp method employed to compare the gene expression in developing anthers between the male sterile and fertile plants of cotton dong a. a cdna clone, designated gharf ( gossypium hirsutum adp - ribosy lation factor ), was isolated from a cotton ( gossypium hirsutum l. ) cdna library using gha27 as probe. 2

    棉花arf基因的克隆選取棉花洞a雄性不育株和可育株花藥cdna - aflp差別顯示的差異片段gha27 ，用地高辛標記作為探針，篩選棉花洞a花藥的cdna文庫，克隆得到了棉花arf基因的cdna全長序列，將其命名為gharf （ gossypiumhirsutumadp - ribosylationfactor ） 。
  17. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  18. To elucidate further the presense and function of integrin - like protein, we try to clone 3 " terminus seqence of integrin - like gene from germinated pollen of lily by 3 " race using degenerate primers corresponding to the conserved cytoplasmic regions of a and 3 subunits

    而要進一步從分子水平上證實類整合素的存在以及研究它的功能，必須克隆其基因。根據動物整合素、亞基的胞質域保守序列設計簡並引物，利用3 race的方法擴增、亞基的3末端序列。
  19. There is no characteristic in the amino acid sequence 63 - 152 and it is the piece that we want to delete to identify the function of the segment. ie180 gene mutants deleted the 64 - 151 amino acid was amplified by muti - pcr and were cloned by pmdist - vector. the clone plasmids were named pjmp1p3p2. the segment corresponding to the sequence of 1 - 1079 amino acid of the genbank sequence amplified by pcr, its clone plasmids was named pjmp1p2. the segment corresponding to the sequence of 454 - 1079 of genbank sequence amplified by pcr and its clone plasmids is named pjmp6p2 - three clone plasmids and pcdna3 were digested by restriction enzyme bamhi and hind, the gene segment of p1p2, p1p3p2, p6p2 were recycled

    本試驗應用dnamis及prosis軟體分別對genbank中登記號為no352564的ie180序列進行了蛋白質序列分析，結果表明其1 - 34段氨基酸序列具有典型helix - turn - helix特徵序列，並且富含酸性氨基酸d及e ，是典型的dna識別序列；富含絲氨酸序列的152 - 409氨基酸序列是一個與激活有關的、潛在的磷酸化位點； 454 - 696氨基酸區域是dna結合域； 64 - 151氨基酸片段沒有明顯的序列特徵；從中可看出ie180蛋白的1 - 1080氨基酸段具有典型的轉錄激活因子結構特徵。
  20. Gene clone and nucleotid sequence analysis of grapevine leafroll virus

    基因的克隆及序列分析
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