gene disruption 中文意思是什麼

gene disruption 解釋
基因打斷,基因破壞
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • disruption : n 分裂，破裂，瓦解；中斷。 the disruption of rock 巖石的破裂。 family disruption 家庭破裂。 The s...
  1. Because the phenotype of osdd2 mutant was segregated with the basta resistance, it suggested that the mutation was arose by disruption of the wrky gene

    根據已有的共分離數據和初步分析結果表明osdd2突變體的發育遲緩性狀是有外源基因插入引起。
  2. Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function

    進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。
  3. Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

    利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
  4. In order to facilitate the study of biological function of add gene cluster, e. coli - s. avermitilis intragenus conjugation system was established. in addition, phz2114 for the replacement of the entire add gene cluster and phz2130 for disruption of adda were constructed

    建立了除蟲鏈黴菌的接合轉移系統，並構建了用於置換全部基因簇的基因置換質粒phz2114和adda的基因中斷質粒phz2130 ，為研究除蟲鏈黴菌add基因簇的生物學功能奠定了基礎。
  5. The complicated life cycle of streptomyccs is one of the most important reason that attracts researchers on its genetics. a great deal of valuable information on the morphological, physiological differentiation of streptomyccs coclicolor will emerge after genome sequencing is completed. based on the s. coelicolor dna sequences that have been finished and focusing on three ftsk homologous, which may be closely related with cell division, this study tried to find out the functions of three ftsk homologues genes by gene replacement or disruption

    鏈黴菌復雜的生活史是研究其遺傳學的一個重要原因，天藍色鏈黴菌全基因組測序將會為其形態及生理分化發育的研究提供大量有用的信息，本研究基於天藍色鏈黴菌已測出的98序列，對一組可能與細胞分裂有關的基因? ftdk同源基因? ?進行基因置換或中斷並對其功能進行初步研究。
  6. Anti - en antibody staining and in situ hybridization on oxt glc embryo using wg antisense probe showed defective en protein and defective wg transcripts bands. the embryonic analysis results indicated that the disruption of oxt gene would lead to the disruption of wg transcription and the expression of engrailed gene, as the wg transcription is dependant on the engrailed

    通過對oxt生殖系克隆胚胎進行的抗en抗體染色和wg反義探針a雜交結果表明，與野生型果蠅的胚胎相比， en蛋白和wg轉錄物條帶都出現了明顯的缺失。
  7. A construct derived from phz2157 is now ready for the constrction of mddl mutant by gene disruption experiment

    在此基礎上構建了用於在mc ~ 2155中進行mddl基因中斷的載體phz2157 。
  8. An internal segment of the whig gene of s. griseus was amplified from plzl through pcr. the 304bp dna fragment was inserted into the ecori / bamhi site of e. coli - streptomyces shuttle plasmid pkc1139, generating pkc1139 : : a whig, named plz107, for gene disruption

    Pcr法克隆whig基因內部304bp片段，連接到大腸桿菌-鏈黴菌穿梭質粒嚇0139 ，構建了基因陽壞用重組質粒賊q139 ： ：凸mg ，命名plz107 。
  9. At present, there are several approaches to targeted gene disruption ( or gene targeting, as it ' s usually called ) that can be customised to attack anywhere in the genome, but they ' re all highly error - prone, disrupting other locations a great deal

    目前，有幾種方法可用於打靶的基因破壞（或稱基因打靶，實際名稱也是這樣） ，它可以定製，用來攻擊基因組的任何地方，但它們全都容易出錯，在非常多的地方破壞其他位點。
  10. The derivatives of phz2104 resulting from the disruption of the three putative orfs respectively by aada can not confer dnd phenotype on zx1. using erase - a - base system, a series of exoiii progressive deletion mutants were prepared for sequencing of add gene cluster

    將add基因簇亞克隆到測序載體pbluescript sk （ + ）上，採用exo缺失突變法得到了66個單向遞減缺失亞克隆，然後對這些克隆進行測序。
  11. Lividans mutant strains la1 and la2 generated by gene replacement in dndb and dndc were obtained respectively. both of these two mutants lost dnd phenotype because of disruption of a respective dnd gene

    首先利用基因置換實驗獲得分別在dndb 、 dndc基因內部發生基因中斷的突變菌株la1利la2 ，並通過southern雜交進行了驗證。
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