gene exchange 中文意思是什麼
gene exchange
解釋
基因交換-
This will promote gene exchange among colonies of the population in unfavorable years and inferior habitats.
這在不利年份和生境較差時，會促進種群集群之間基因的交換。 -
Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。 -
Because callow birds had not be marked with rings in the early period of protection, the pedigree was confused. so, it is very difficult to carry out artificial pairing. for its reference value to set reasonable breeding plan, the pedigree chart constructed by us can be applied in the protection to crested ibis. according to a good breeding project, inbreeding can be avoided and gene exchange can be increased
由於在朱?保護的初期階段未能對離巢幼鳥進行環志，故現存朱?的譜系關系混亂，給人工選種配對帶來了困難；我們所構建的譜系圖可以在一定程度上應用到西北大學碩士學位論文沐潭鳥保護的實際工作中，為合理地制定繁殖考。 -
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 -
It is necessary for a species " adaptation and evolutionary potential to keep genetic variety at a relative level. genetic diversity in the population must not be lost furthermore and gene exchange among individuals should be enhanced as much as possible to avoid extinction of crested ibis
一定程度的遺傳變異性是維持種群的適應力與進化潛力的必要條件，為使朱?免於滅絕的厄運，必須避免其種群遺傳多樣性的進一步喪失，並盡可能增加個體間的基因交流，最大限度地避免近親繁殖。 -
This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。 -
The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction. marker exchange method was used to study the relationship between the production of protease and the 3 orfs. a deletion mutant of xcc _ 4463 was constructed successfully
Orf注釋表明，共中一個基因為胞外蛋白酶結構基因， 3個基因與合成調控有關， 5個與轉運分泌有關， 2個與lps合成有關，其餘3個orf的注釋功能與胞外蛋白酶的關系未見報道，為研究這3個orf與胞外蛋白酶產生的關系，採用同源雙交換orf缺失法進行了進一步驗證，成功地構建了xcc _ 4463缺失突變體，所得缺失突變體經檢測胞外蛋白酶減少，在寄主上致病性降低。 -
The unequal exchange of dna in a reproductive cell and subsequent mutation of an extra copy of a gene for a pigment sensitive to long wavelengths resulted in the creation of a second long - wavelength - sensitive pigment, which had a shift in its wavelength of maximum sensitivity
生殖細胞的dna不對等互換，以及長波長色素重復基因的后續突變，創造出了第二種對長波長敏感的色素，只不過最敏感的波長已經變了。 -
This type of natural gene exchange is safe and fairly predictable
這種自然的基因交換既安全，又具有相當的可預見性。
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