gene expression system 中文意思是什麼

gene expression system 解釋
基因表達系統
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  • system : n 1 體系，系統；分類法；組織；設備，裝置。2 方式；方法；作業方法。3 制度；主義。4 次序，規律。5 ...
  1. Objective to investigate the effects of fluid shear stress on il - 8 gene in human umbilical vein endothelial cells ( huvecs ) and the roles of time course of low shear stress and intensity of fluid shear stress using lightcycler ? system ; to investigate the gene expression profiles in huvecs exposed on low shear stress ( 4. 20 dyne / cm2, 2 h ) and incubated by 17 p - estradiol ( 10 - 7 m ) + low shear stress ( 4. 20 dyne / cm2, 2 h ) using the cdna microarray approach. methods endothelial cells were isolated from human umbilical cord veins by collagenase treatment as described by jaffe and modified

    目的探討人臍靜脈內皮細胞（ humanumbilicalveinendothelialcells ， huvecs ）在流體切應力作用下il - 8基因的誘導表達以及切應力的作用時間和作用強度對il - 8基因表達影響的變化規律；利用表達譜基因晶元技術研究內皮細胞在低切應力（ 4 . 20dyne cm ~ 2 ， 2h ）作用下其切應力相關基因的表達情況以及生理濃度（ 10 ~ （ - 7 ）的17 -雌二醇孵育內皮細胞48h ，再經同樣條件的切應力處理后對內皮細胞切應力相關基因表達的可能影響。
  2. With the successful expression of exogenous gene in plant. the advantage of plant expression system become a highlight increasingly. based on the successful expression of hbmp - 3m gene into tobacco, we maked the study o f transferring hbmp - 3m gene into tomato and hbmp - 3 gene into tobacco, in order to obtain tomato transgenic plant with hbmp - 3m gene and tobacco transgenic plant with hbmp - 3 gene, to establish basis for getting step farther of leaning the expression of hbmp - 3m gene and hbmp - 3 gene in plant, the difference between the product of hbmp - 3m gene and hbmp - 3 gene in plant and the active of expressible product

    隨著植物基因工程的迅猛發展，一些疫苗、抗體、細胞因子等異源蛋白在植物中的成功表達，植物表達系統的優點日益受到關注。本研究室在成功地將hbmp - 3成熟肽基因轉入煙草並檢測有目的蛋白表達的基礎上，進行hbmp - 3成熟肽基因轉化番茄和hbmp - 3全長基因轉化煙草的研究，以期獲得轉hbmp - 3成熟肽基因番茄和轉hbmp - 3全長基因煙草植株，為進一步研究hbmp - 3成熟肽基因和全長基因在植物體內的表達及區別奠定基礎。
  3. Since 1980s he has been studying the mechanism of normalization of abnormal blood pressure and the resetting of baroreceptor reflex by acupuncture and somatic nerve stimulation, effects of brain osmoreceptor stimulation on sympathetic nerve discharge and renal activity, the role of vasopressin in body fluid and salt homeostasis, difference in gene expression between hypertensive and normotensive rats, hypertension and cardiac ischemia - induced myocardial remodeling, effect of steroids on the nervous system, etc. with his research achievements, dr. yao was awarded science and technology progress prize sponsored by the state education commission grade a award in 1986, grade b award in 1995 and by the ministry of health grade b award in 1989 and 1993, grade a award of guang - hua science and technology prize 1995, and chinese universities science and technology prize grade b award in 2000

    近二十年來，他主要從事針刺和刺激軀體神經對心血管和腎臟活動的調整作用刺激腦內滲透壓感受器引起的交感神經放電和腎臟活動的改變血管升壓素在心血管活動調節和水鹽平衡中的作用原發性高血壓的相關基因高血壓和心肌梗死引起的心肌重構甾體激素對神經系統的影響等。他的科研成果曾獲得國家教委科技進步獎一等獎一項1986年二等獎一項1995年衛生部醫藥衛生科技進步獎二等獎二項1989 1993年光華科技基金獎一等獎一項1995年中國高校科學技術獎二等獎一項2000年。
  4. The two cdna fragments, ap - 2a full length cdna and ap - 2 a cdna fragments were inserted in frame into gst gene fusion system. with an ap - 2a monoclonal antibody we detected the expression of gst - ap - 2a

    將這兩段篩選所得cdna片段和ap - 2 cdna全長及ap - 2 cdna的分段形式用gst基因融合系統進行表達，並用ap - 2的單克隆抗體檢測了gst - ap - 2融合蛋白表達的情況。
  5. They are also the key components of insect - baculovirus expression system ( bevs ), which has been one of the four main expression systems in gene engineering

    同時，桿狀病毒昆蟲細胞表達系統是一類重要的真核表達系統，現已成為目前基因工程四大表達系統之一。
  6. The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses

    同時，還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試，並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學，細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞，但其遺傳學和細胞生物學背景知之甚少。
  7. It has been used to express in an attractive level a variety of intracellular and extracellular proteins of interest. ln our study, pichia pastoris gene expression system was used to express the soluble part of human trail ( tnf related apoptosis induced ligand ) and antibacterial peptide - ceme

    因此它在基因工程方面的應用越來越受到人們的重視，已被廣泛應用於一系列胞內及胞外蛋白的高水平表達。我們利用這個系統分別對人的trail （ tnfrelatedapoptosisinducedligand ， trail acl2 ）基因可溶性部分和抗菌肽ceme基因的表達進行了研究。
  8. Serial studies on relationship between nucleotide excision repair system gene expression and chemotherapeutic

    核苷酸切除修復系統基因表達水平與化療藥物耐藥相關性的系列研究
  9. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。
  10. The genetic regulation of stomatal movement mainly depends on an efficient control system of gene expression, and guard cell - specific promoter is becoming one of the best choices

    因此有必要對氣孔的開關進行調控。氣孔運動的分子調控離不開高效的基因開關系統，保衛細胞特異性表達的啟動子近幾年倍受關注。
  11. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  12. Construction of a high - effective expression system of bacillus subtilis recombined xylanase gene

    幾丁質結合區三個結構功能域的鏈黴菌
  13. 4. the wild tomato and nr mutant fruit discs treated with wound, nacl and peg to detect expression changes of le - acs gene family. the experiment showed that during the ethylene production under stress conditions, the le - acs2 gene expression maintained at a high level, different from system i ethylene production transition to system ii. this suggested that stress conditions changed the regulatory mechanism of le - acs2

    傷、 nacl 、 peg處理普通型番茄和nr突變體番茄的組織圓片研究le - acs基因家族的表達變化，結果表明：在脅迫乙烯生成過程中， le - acs2一直呈高水平表達，不存在如同系統向系統乙烯的轉化中le - acs基因家族的表達過渡，因此，脅迫條件下le - acs2基因的轉錄調控機制發生改變。
  14. It has not only many advantages of the prokaryotic expression system such as growing quickly, manipulating easily, strong power to express and control foreign protein, but also the ability to make foreign protein modified in post - translation. so pichia pastoris gene expression system is more appropriate to express many eukoryotic proteins than prokaryotic system and has attracted more and more people " s attention on it

    它不僅具有原核表達系統生長快速，操作簡單，外源基因表達量大並受到嚴格調控等特點，還具有真核生物特有的蛋白質翻譯后修飾的功能，表達真核生物蛋白質具有原核表達系統無法比擬的優越性。
  15. In addition, we also focused on constructing a plant virus - based gene vector pvx to express ltb - st fused gene by means of agroinfection. the possibility of producing vaccine was probed through plant virus - based gene vector expression system

    同時本研究還通過構建ltb ? st融合基因pvx病毒表達載體，並用農感染法轉化植物，探索了暫態表達系統生產疫苗的可能性。
  16. A method based on gene expression programming ( gep ) for identifying the nonlinear system model is presented, which makes up the insufficiency that traditional identification methods need much a priori information, and has a tidier and more efficient system model expression mode than genetic programming ( gp )

    摘要給出了利用基因表達編程（ gep ）進行非線性系統辨識的方法，彌補了傳統辨識方法需要過多預知信息的不足，有著比遺傳編程（ gp ）更簡潔有效的系統模型結構表達方式。
  17. These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5

    方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ e
  18. Advances of studies on the involvement of the second messenger system and gene expression in the effect of acupuncture

    第二信使系統和其介導的基因表達對針灸效應影響的研究
  19. Upregulation of gene expression of adrenomedullin and its receptor system in calcified vascular smooth muscle cells

    鈣化血管平滑肌細胞腎上腺髓質素及其受體系統的基因表達上調
  20. Pichia pastoris gene expression system is a second generation expression system in yeast after the s. cerevisiae

    甲醇畢赤酵母pichiapastoris表達系統是繼釀酒酵母之後的第二代酵母表達系統。
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