gene marker 中文意思是什麼

gene marker 解釋
基因標記
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • marker : n 1 作記號的人；打分數的人；記分器；劃線器；指示器；【無線電】指點標；（撞球等的）記分員。2 【軍...
  1. A pcr - based dna marker for a - gliadin gene in common wheat

    醇溶蛋白基因的分子標記
  2. Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker

    本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。
  3. Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions

    本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。
  4. Transgenic plants and their marker gene knock - out

    轉基因農作物及其標記基因的消除
  5. We designed one primer pairs ctb - 1, ctb - 2 to amplify about 580bp of cyt b gene sequence as a molecular marker to analyze phylogenetic relationship of 14 species of oedipodidae in china. dna sequences were aligned using clustal x, followed by refinement by eye based on the corresponding deduced amino acid sequences. after cutting off 5 " and 3 " termini unaligned sequences, we get 462 bp segment

    本研究從分子生物學角度入手，採用cytb作為分子標記，採用自行設計的一對cytb基因特異引物ctb - 1 、 ctb - 2 ，通過pcr技術，共獲得斑翅蝗科4個亞科14個種的代表個體以及癩蝗科1個種的代表個體的580bp左右的cytb部分序列。
  6. We discussed the advances of research on resistant breeding for frogeye leaf spot of soybean from three aspects, resistant breeding, resistant germplasm screening and marker on resistant gene for frogeye leaf spot, which could provide information for resistant breeding

    摘要從抗灰斑病育種、抗性資源篩選和抗灰斑病基因分子標記三個方面闡述了國內外抗大豆灰斑病育種的研究進展，為抗灰斑病育種者提供可參考的相關信息。
  7. Sinense y. x. lin using allzyome marker. the experiment employ six enzymes : est, mdh, fdh, gdh, sod and acp, using their polymtic locus to analyse the allele gene frequency, the number of the effective allele gene

    採用est 、 mdh 、 fdh 、 sod和acp等5種酶系統，分析荷葉鐵線蕨的等位酶遺傳變異，用間接法估算荷葉鐵線蕨交配系統的特徵參數? ?異交率t 。
  8. The isozyme took one kind of important genetic marker is widely applied to biology research each domain, the plant isozyme can in the very great degree be able to reflect between the plant individual the heredity difference, is surveys the gene difference and the hereditary change one important method

    同工酶作為一種重要的遺傳標記被廣泛應用於生物學研究的各個領域，植物同工酶能夠在很大程度上能反映植物個體之間的遺傳差異，是探測基因差異和遺傳變異的一種重要手段。
  9. Lea3 gene probe and marker probe were labeled by dig - chem - link

    Okblea3片段，並用地高辛標記法進行了lea3基因探針的制備。
  10. Identification and marker - assisted selection of hmw - glutenin 1dx5 gene in wheat

    技術研究中國特有小麥種子貯藏蛋白基因的遺傳變異
  11. Three chloroplast transformation vectors including pds16s - cat, ptn1269 - bar and psp72 - n5 - bar - n3 were constructed, using ! 6s rrna or chln gene sequence as a homologous segment and cat or bar as a selective marker gene, respectively. foreign genes were introduced to the cells of d. salina by microprojectile bombardment method and a pilot chloroplast tran

    3 .杜氏鹽藻葉綠體165出na基因的克隆和轉化載體的構建根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，克隆了杜氏鹽藻葉綠體16srrna基因部分序列1100bp ，並利用克隆的16srrna鄭州大學2003年博士學位論文
  12. ( 2 ) the interest gene in pcar is under the control of acti which is the strong promoter in rice. the selectable marker gene is hyg gene

    （ 2 ）表達載體pcar中目的基因由單子葉強啟動子水稻act1啟動子調控，選擇標記基因為潮黴素抗性基因。
  13. The ms188 gene was finely mapped. a total of 8 new indel markers were designed to map msl88 using a segregating population with a total of 2135 male sterile progenies. ms188 was finally mapped to a region of 95. 8kb between the molecular marker mda7 and k24c1

    在與ms188連鎖的分子標記mc015附近設計了8個indel分子標記，對遺傳群體中2135株不育植株進行基因型分析，最後將目的基因定位於第五條染色體分子標記mda7和k24c1之間95 . 8kb的區間內。
  14. Study on a stripe resistance gene and its molecular marker in wheat ica31 from syria

    31抗條銹病基因分析及分子標記研究
  15. Z which involve in melanin, tyrosinase related protein 1 gene ( tyrpi ) and dermal melanin inhibitor gene ( id ). the genomic structure of tyrp1 was determined and its correlation between melanin accumulation and tyrp1 expression was studied. moreover, we constructed a bac contig near id locus which was on a region short of dna marker in chr. z

    利用構建的bac文庫對z染色體上黑色素相關基因酪氨酸酶相關蛋白1基因（ tyrp1 ）的基因結構以及該基因的表達與黑色素沉積的關系進行了研究，同時構建了位於z染色體上缺乏標記區段的表皮黑色素抑制因子基因（ id ）的bac重疊群。
  16. Green fluorescent protein is widely applied in researches of modern life science, such as gene product moved - process in vivo, protein localization, drug screening and preliminary selection of transgenic individual as a molecular marker

    綠色熒光蛋白這種獨特的生物學特性，使其作為報道基因在現代生命科學研究領域中，如細胞內基因產物的動態過程，蛋白質在細胞內的定位，藥物的篩選，以及轉基因個體的初步鑒定等等有著廣泛的應用。
  17. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  18. No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract

    將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。
  19. Research field were transgenic breeding, marker assisted breeding, molecular design breeding, related plant functional gene, genetic diversity, molecular maker genetic, basic theory and experiment technology of genetic breeding

    研究領域主要涉及轉基因育種、分子標記輔助育種、分子設計育種以及相關的植物功能基因、遺傳多樣性、分子標記遺傳及遺傳育種基礎理論和實驗技術等。
  20. Location of the sterile gene for female sterile wheat by microsatellite marker

    小麥雌性不育基因的微衛星標記定位
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