gene order 中文意思是什麼
gene order
解釋
基因序列-
Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。 -
With the successful expression of exogenous gene in plant. the advantage of plant expression system become a highlight increasingly. based on the successful expression of hbmp - 3m gene into tobacco, we maked the study o f transferring hbmp - 3m gene into tomato and hbmp - 3 gene into tobacco, in order to obtain tomato transgenic plant with hbmp - 3m gene and tobacco transgenic plant with hbmp - 3 gene, to establish basis for getting step farther of leaning the expression of hbmp - 3m gene and hbmp - 3 gene in plant, the difference between the product of hbmp - 3m gene and hbmp - 3 gene in plant and the active of expressible product
隨著植物基因工程的迅猛發展，一些疫苗、抗體、細胞因子等異源蛋白在植物中的成功表達，植物表達系統的優點日益受到關注。本研究室在成功地將hbmp - 3成熟肽基因轉入煙草並檢測有目的蛋白表達的基礎上，進行hbmp - 3成熟肽基因轉化番茄和hbmp - 3全長基因轉化煙草的研究，以期獲得轉hbmp - 3成熟肽基因番茄和轉hbmp - 3全長基因煙草植株，為進一步研究hbmp - 3成熟肽基因和全長基因在植物體內的表達及區別奠定基礎。 -
G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。 -
Recently, we know the number of muntjac deer chromosomes varies from 6 to 48, and there are karyotypic polymorphism intra - species relationships. these animals are extremely useful for phylogenetic studies. in order to discuss the phylogenetic relationships of muntiacinae, we have studied the nuclear gene sequence of muntiacus muntjak, muntiacus crinifrons, muntiacus reevesi, and elaphodus cephalophus
為了探討麂亞科動物間的親緣關系，本論文以赤麂（ muntiacusmuntjak ） 、黑麂（ muntiacuscrinifrons ） 、小麂（ muntiacusreevesi ）和毛冠鹿（ elaphoduscephalophus ）這4種麂亞科動物為材料，以核基因序列為研究對象，作進一步的研究。 -
In order to benefit from this new technique without any dangers, on the basis of the arguable contents, the paper put forward a series of principals with the knowledge of systematical philosophy, mathematics and ecological. agriculture : welfare principal, invariable principal of gene, non - spread principal of gene, equal principal of substance and informed perfectible principal. and then we establish a model of evaluating the social effects of gmo
為了從這種新技術成果中獲得好處，同時又不會帶來風險，本文以爭論的內容為基礎，從轉基因作物能比傳統農作物帶來更多的社會效益的角度出發，運用系統哲學、生態農業和數學的一些知識，提出了轉基因作物研究和推廣中遵循的一系列原則：福利原則、基因穩定性原則、基因不擴散原則、實質等同性原則、以及知情選擇原則，並進而建立起評價具體的轉基因作物的社會效益的模型。 -
Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique
本研究的特色和創新之處在於，針對羅氏沼蝦不耐低溫，但體型相對較大，精莢明顯的特點，首次將目前已知具有最強抗凍活性的雲杉卷葉蛾（ sprucebudworm ， choristoneurafumiferana ）抗凍蛋白（ sbwafp ）基因（ sbwafp ） ，通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中，以期培育出耐低溫的羅氏沼蝦新品系。 -
3. the effect of sporulation - independent promotor on toxicity of natural strain in order to study the effect of sporulation - independent promotor ( p3a ), p3a was spliced with the cry1c gene, then inserted into the shuttle vector pht304, and then recombinated plasmid pbmb827 was obtained. after transferring pbmb827 into strain ybt - 1520, it was surprising that the transformants had almost no potency against all lepidopteran larvae tested
3非依賴芽胞形成icp的cry3a啟動子（ p _ （ 3a ） ）對野生菌株特性的影響帶p _ （ 3a ）和cry1c基因的重組質粒pbmb827轉入ybt - 1520 ，轉化子對所測昆蟲的毒力下降非常明顯，芽胞和晶體也很難脫落。 -
In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate
將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。 -
Meanwhile, in order to improve the e. coli with ability of using sugar. we have recombined the vecter of parg25 a which have ability of using sugar with the gene of sucrase length of 7800bp
同時，為了促進所構建的大腸桿菌具有蔗糖利用能力，我們構建了含有大小為7 . 8kb的蔗糖水解酶基因sacc重組質粒，篩選得到含有兩種基因的重組子，並轉化大腸桿菌，使大腸桿菌獲得蔗糖利用能力。 -
In order to facilitate the study of biological function of add gene cluster, e. coli - s. avermitilis intragenus conjugation system was established. in addition, phz2114 for the replacement of the entire add gene cluster and phz2130 for disruption of adda were constructed
建立了除蟲鏈黴菌的接合轉移系統，並構建了用於置換全部基因簇的基因置換質粒phz2114和adda的基因中斷質粒phz2130 ，為研究除蟲鏈黴菌add基因簇的生物學功能奠定了基礎。 -
In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not
合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。 -
Css is the one of the colonization factor antigens which is a protective antigen can cause immune reaction by the means taking orally. in this study, carrot was separately transformed with agrobacterium tumefacience strain lba4404 that contains ctb or ctb - cs3 fused gene in order to get oral diarrhea vaccine. this is a potent strategy to produce etec oral vaccine
本研究期望通過農桿菌轉化系統將ctb （霍亂毒素b亞基，可作為佐劑和載體）基因和ctb - cs3融合基因分別轉入胡蘿卜植株，使ctb和ctb ? cs3融合基因穩定整合到胡蘿卜基因組內，希望以此獲得以胡蘿卜為受體的etec口服疫苗，使服食者在進食的同時就可獲得腹瀉免疫。 -
Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。 -
The experimental procedure that begins with a cloned segment of dna, or a protein sequence, and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function
在已知dna克隆片段或蛋白質序列上引導程序性的變異（通過直接誘變）並返回到基因組，來研究基因和蛋白質功能的實驗方法。 -
Secondly, analysis of peroxidase isoenzyme with polyacrylamidedel electrophoresis for was performed in order to investigate the changes of gene expression under sound stimulation. it could be seen from electrophoresis gel that each group had 6 enzyme bands. new enzyme band in pod electrophoretogram was n ' t detected for stressed groups
此外，在部分實驗組的培養基中加入不同濃度的蛋白質合成抑制劑環己亞胺酮（ chm ）后發現， pod和cat的活性有所降低，暗示著聲波處理使保護酶活性升高的原因可能是聲波處理促進了細胞內酶的合成。 -
In order to solve the default of the limited valid scope and discontinuity characteristic, introducing the state continuous conversion gene based on establishment of respective mathematics model for low and magnitude current scope, and integrate with them reasonably then having established the mathematics model of suitable whole scope and having solved present mathematics model existent problems
本文針對目前電弧爐負載數學模型存在的適用范圍有限、特性不連續等缺點，在建立了分別適合大、小電流區域運行特性的數學模型的基礎上，引入狀態連續轉換因子，將分別適合大、小電流區域運行特性的數學模型有機結合為一個整體，建立了適合全范圍的數學模型，解決了目前數學模型存在的問題。 -
In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera, the ecori - n fragment located at 54. 8 - 59. 3 kbp of the viral genome was sequenced. the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda
對棉鈴蟲單核衣殼核多角體病毒（ helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus ， hasnpv ）基因組中ecori ? n片段進行序列分析，獲得了完整的解螺旋酶基因（ hel ） ，其開放閱讀框大小為3762bp ，編碼一個分子量為146kda的蛋白質。 -
Rearrangements have been occurred in at least twelve mitochondrial genes, which have drastically altered the gene order of the mitten crab e. japonica sinensis to the putative ancestral pancrustacean ( crustacean / insecta ) gene order
中華絨螯蟹線粒體基因組與推測的泛甲殼類原始的線粒體基因順序相比，至少有12個基因發生了重排，造成線粒體基因排列順序顯著的變化。 -
Dna sequences of the entire mitochondrial genome from mammals and african clawed frog suggested that all vertebrates shared a common gene order
從非洲爪贈到人類的線粒體基因組都具有共同的基因順序。 -
4 gene rearrangements and proposed mechanisms of vertebrate mtdna vertebrate mitochondrial gene order was initially considered completely conserved
4脊椎動物線粒體基因重排及機制脊椎動物線粒體基因順序最初認為是完全保守的。
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