gene recombination technology 中文意思是什麼

gene recombination technology 解釋
基因重組技術
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • recombination : 沖調；復水
  • technology : n. 1. 技術，工程，工藝。2. 製造學，工藝學。3. 術語（匯編）。
  1. Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress

    最近的研究表明，基因轉移與重組技術在結縷草遺傳改良中具有巨大應用潛力；結縷草遺傳連鎖圖譜構建取得重要進展；基因克隆和基因資源研究正在展開。
  2. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  3. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  4. The results were as following : 1. construction and identifcation of recombinant plant expression vector pbi ! 2i - th by dna recombination technology, sweet protein thaumatin gene was cloned into plant expression vector pbii2i. recombinant plasmid pbii2i - th was constructed successfully by enzyme cutting and electrophoresis

    甜蛋白thaumatin基因植物表達質粒pbi _ （ 121 ） - th的構建與鑒定利用dna重組技術，將植物甜蛋白thaumatin基因克隆至植物表達載體pbi _ （ 121 ）中，通過酶切、電泳，鑒定thaumatin基因已成功構建到植物表達質粒pbi _ （ 121 ）中。
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