gene segment 中文意思是什麼

gene segment 解釋
基因節段
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • segment : n 1 (自然形成的)段落;斷片;部分;分節;段;節。2 【數學】(線)段;弓形。3 圓缺;球缺。4 環節...
  1. Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits. after reverse transcription, cdna was amplified using specific primers for each gene segment

    用qiagen的rneasyrna試劑盒提取病毒rna后,逆助興才李醫『筍脂碩聖『老『才轉錄合成cdna 。
  2. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株( agrobacteriumtumefaciens ) ,含質粒pyhy (目的基因及npt 、 gus基因) ,對白樺試管苗莖段,葉柄,葉片三種外植體進行侵染,結果表明:大葉片生長勢強,為轉基因的最優外植體,轉化率能夠達到22 。
  3. Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine

    結果顯示:擴增的cdna片段長度為289bp ,其中含有一個編碼45個氨基酸的開放閱讀框,閱讀框所編碼的氨基酸中含有10個半胱氨酸,且在序列中均排列成cys - x - cys ,其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ,擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ,這一點也和果蠅mtn基因十分相似。
  4. Described here is the cloning and characterization of a new trichome - specific - promoter in arabidopsis. by ddrt ( differential display reverse transcription ) - pcr and reverse northern, a 280bp sequence is acquired from the epidermal in viciafaba, which is specially expressed in the epidermal. then using race, we abtain a 3. 0 kb gene segment including the 3 ' end full sequence and the 5 ' end partial sequence of the 280bp segment

    首先,我們利用差異顯示技術和反northern技術,以蠶豆葉肉細胞為對照,從蠶豆葉片表皮細胞中克隆出長280bp的新的表皮特異表達基因片段,再通過race技術獲得此基因片段的3 』端全序列和5 』端部分序列。
  5. We designed one primer pairs ctb - 1, ctb - 2 to amplify about 580bp of cyt b gene sequence as a molecular marker to analyze phylogenetic relationship of 14 species of oedipodidae in china. dna sequences were aligned using clustal x, followed by refinement by eye based on the corresponding deduced amino acid sequences. after cutting off 5 " and 3 " termini unaligned sequences, we get 462 bp segment

    本研究從分子生物學角度入手,採用cytb作為分子標記,採用自行設計的一對cytb基因特異引物ctb - 1 、 ctb - 2 ,通過pcr技術,共獲得斑翅蝗科4個亞科14個種的代表個體以及癩蝗科1個種的代表個體的580bp左右的cytb部分序列。
  6. The experimental procedure that begins with a cloned segment of dna, or a protein sequence, and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function

    在已知dna克隆片段或蛋白質序列上引導程序性的變異(通過直接誘變)並返回到基因組,來研究基因和蛋白質功能的實驗方法。
  7. Three chloroplast transformation vectors including pds16s - cat, ptn1269 - bar and psp72 - n5 - bar - n3 were constructed, using ! 6s rrna or chln gene sequence as a homologous segment and cat or bar as a selective marker gene, respectively. foreign genes were introduced to the cells of d. salina by microprojectile bombardment method and a pilot chloroplast tran

    3 .杜氏鹽藻葉綠體165出na基因的克隆和轉化載體的構建根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料,克隆了杜氏鹽藻葉綠體16srrna基因部分序列1100bp ,並利用克隆的16srrna鄭州大學2003年博士學位論文
  8. A gene is a short segment of dna

    一個基因是dna (脫氧核糖核酸)上面的一個小段。
  9. Gene - a functional unit of heredity which is a segment of dna located in a specific site on a chromosome

    基因位於染色體特定部位代表遺傳功能單位的dna片斷。
  10. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接;用連接產物轉化大腸桿菌jml09感受態細胞,置含amp 、 x - gal和iptg的lb平板上培養12 20小時;挑取白色菌落於選擇性培養基擴大培養,堿裂解法小量提取質粒dna ,並進行酶切分析鑒定,結果獲得整合有prvge基因的重組質粒pugedna ,並與其它prv分離株進行ge基因序列同源性分析。
  11. There is no characteristic in the amino acid sequence 63 - 152 and it is the piece that we want to delete to identify the function of the segment. ie180 gene mutants deleted the 64 - 151 amino acid was amplified by muti - pcr and were cloned by pmdist - vector. the clone plasmids were named pjmp1p3p2. the segment corresponding to the sequence of 1 - 1079 amino acid of the genbank sequence amplified by pcr, its clone plasmids was named pjmp1p2. the segment corresponding to the sequence of 454 - 1079 of genbank sequence amplified by pcr and its clone plasmids is named pjmp6p2 - three clone plasmids and pcdna3 were digested by restriction enzyme bamhi and hind, the gene segment of p1p2, p1p3p2, p6p2 were recycled

    本試驗應用dnamis及prosis軟體分別對genbank中登記號為no352564的ie180序列進行了蛋白質序列分析,結果表明其1 - 34段氨基酸序列具有典型helix - turn - helix特徵序列,並且富含酸性氨基酸d及e ,是典型的dna識別序列;富含絲氨酸序列的152 - 409氨基酸序列是一個與激活有關的、潛在的磷酸化位點; 454 - 696氨基酸區域是dna結合域; 64 - 151氨基酸片段沒有明顯的序列特徵;從中可看出ie180蛋白的1 - 1080氨基酸段具有典型的轉錄激活因子結構特徵。
  12. A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4. 1 softwarre, they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain, therery, they serve mutually as template and primer, and their extension were carried out to synthesize 90 bp " gnrh / trs

    本研究根據genbank中已發表的人gnrh2基因mrna序列以及轉運肽( transporter , trs )基因核苷酸序列,藉助oligo4 . 1設計了一對寡核苷酸引物,以引物3末端的短互補序列退火形成小段雙鏈,從而互為模板和引物,通過引導合成長達90bp的gnrh trs序列gnrh trs 。
  13. Up to the mid - 1970s, it was thought that a gene was one single continuous segment of the dna strand - until it was discovered that in higher organisms such as ourselves a gene can consist of several different segments that have to splice themselves together like pieces of edited movie film before they can pass on their information in the growth of new cells

    一九七零年代中以前,世人以為基因是一個完整而無間斷的dna片段;但后來發現,人類及其他高等生物的基因由幾個片段構成,要像剪接電影一樣併合起來,才能在新細胞生長過程中傳遞訊息。
  14. We have cloned a segment of rci - 1 gene by means of rt - pcr and made it as a probe. the effects on the molecular level of the single chemical reagents and the mixed chemical reagent were contrasted under non - stress and different stresses, such as chilling, high - salt and pathogen infection. it was shown that on the condition of non - stress, the control had no expression of rci - l all the time

    採用rt - pcr的方法克隆得到其片段,製作成相應的探針,檢測在非逆境條件下及逆境條件下(包括低溫、鹽害、病害) ,適宜濃度的sa單劑、 ipt單劑以及sa與ipt的復劑對rci - 1基因表達狀況的影響。
  15. According to the conserved domain of the pal gene family, we achieved to the segment of pal gene about 950bp in rhodiola crenulata. some proposals were put forward for further development of the important enzymes in the metabolic pathway of salidroside

    我們還根據苯丙氨酸解氨酶( pal )基因家族的保守序列,以大花紅景天dna為模板,通過pcr擴增得到其約950bp長的pal基因的克隆,測序並比較了它與其基因家族的同源性。
  16. Phylogenic tree from 29 strains were draw based on the ndv hn gene ( part segment ), and the result is similar to that of f gene

    根據29株ndvhn基因部分編碼序列繪制ndv系統發育進化樹,發現f及hn基因的分型結果大致相同。
  17. An internal segment of the whig gene of s. griseus was amplified from plzl through pcr. the 304bp dna fragment was inserted into the ecori / bamhi site of e. coli - streptomyces shuttle plasmid pkc1139, generating pkc1139 : : a whig, named plz107, for gene disruption

    Pcr法克隆whig基因內部304bp片段,連接到大腸桿菌-鏈黴菌穿梭質粒嚇0139 ,構建了基因陽壞用重組質粒賊q139 : :凸mg ,命名plz107 。
  18. Arginine rich human histone h3 is a kind of basic nucleosome protein. in the medium of physiological condition, by electrostatic interaction, histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge. in this paper, in order to establish a new technology of transfection, the functional domains la and ii gene segments of pea were lined with histone h3 gene segment, then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis

    本實驗在已成功獲得的pea功能區a ,功能區基因片段與人組蛋白h3基因片段的克隆菌株的基礎上,構建原核表達載體,將克隆質粒pmd - 18t - pea經nco , mlu雙酶切,回收酶切產物;將克隆質粒pmd - 18t - h3經mlu , xho雙酶切,回收酶切產物。
  19. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段,從c ta基因5 』端第94位到500位核苷酸段設計引物,目的片段407bp ,覆蓋第116和188位兩個aug密碼子,也包含了第一外顯子和第二外顯子間的剪接位點:用常規分子生物學方法構建了反義片段的腺病毒表達載體( padeasy - 1系統) ;腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒,用氯化銫密度梯度離心法獲得純化的高滴度腺病毒;進行體外基因轉移,分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞,觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用,從而達到調控mhc -類分子表達的目的。
  20. Gene duplication is defined as segment of dna duplicate one or more copies, it is believed to be a major mechanism for the generation of evolutionary novelty

    摘要基因倍增指基因組中含有基因的dna片段復制出一個或更多拷貝的過程,是進化出新物種的主要原因。
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