gene transfection 中文意思是什麼

gene transfection 解釋
基因轉染
  1. The protective effect of bcl - 2 gene transfection on heat stressed cardiomyocytes

    2基因轉染對熱應激心肌細胞的保護作用
  2. Protective effect of c - fos antisense gene recombinant transfection on ischemic hypoxic cardiomyocytes

    反義基因轉染對缺血缺氧心肌細胞的保護作用
  3. The recombinant baculovirus harboring the appa gene was obtained after co - transfection and several screening rounds

    用乳糖誘導使appa基因在大腸桿菌菌株bl21中得到了高效表達。
  4. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  5. Are the changes in signal transduction and gene expression related to the structural alteration of the sugar chain on some surface receptors resulting from the transfection with sense or antisense gnt - v

    而細胞信號轉導的改變是否與細胞表面某些受體的糖鏈結構的改變（由gnt - v的正義或反義cdna轉染引起的）有關
  6. Direct damage on supf trna gene can be neglected because half - life of mnng is 1. 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours. therefore the mutagenesis is not lesion directed

    20m的mnng經洗滌后的極微量殘留經過10 ? 20多個半衰期的降解后，己不足攻擊轉人的qdna分子，因此這種突變顯然是發生在mnng直接攻擊部位以外的正常堿基上。
  7. Improvement of adcmv - gfp gene transfection efficiency induced by heavy - ion beam irradiation on murine melanoma cells

    離子束輻射對用帶有綠色熒光蛋白基因的缺陷性腺病毒
  8. Restin was a novel gene isolated by our lab from differentiation tumor cell induced by all - trans retinoic acid in 1999, and its molecular functions were not well understood. it mainly expresses in terminally differentiated cells. by transfection of restin into tumor cell, the cells were arrested in g1 phase and the cell proliferation was inhibited

    Restin是1999年本室在研究全反式維甲酸誘導細胞分化時克隆得到的一個人類未知功能的新基因，該基因主要表達于終末分化細胞，穩定轉染該基因的細胞出現g1期阻滯，抑制細胞的增殖，提示restin與細胞周期的調控有關。
  9. The common inducing protocols, including specific inducers, five - step induction, stromal cell coculture and gene transfection, are not very effective, with the shortcomings of complicated manipulation and high costs

    目前常用誘導方法包括使用特異性誘導劑、 「五步誘導法」 ，還有尚有爭議的基質細胞共培養法，以及基因轉染與篩選，但有操作復雜、費用高、毒性大等缺點。
  10. Expression of target gene in mesenchymal stem cells after transfection of basic fibroblast growth factor gene

    堿性成纖維細胞生長因子基因轉染大鼠骨髓間充質幹細胞后目的基因的表達
  11. Transplantation of allogenetic bone marrow mesenchymal stem cells combined with vascular endothelial growth factor gene transfection for treating myocardial infarction

    同種異體骨髓間質幹細胞移植聯合血管內皮細胞生長因子基因轉染治療心肌梗死
  12. Gene therapy of tumor using telomerase as a target is a prospective new approach with tremendous potentials. 2. transfection of telomerase positive sense expression plasmid plncx - tr into human normal peripheral leukocytes did n ' t necessarily change the level of telomerase activity in cells, it indicated that expression of exogenous htr gene could not activate the expression of htert and increase telomerase activity

    將正義重組質粒plncx - tr ，在體外導入人體正常外周血白細htr基因在細胞中的表達及檢測端粒酶活性方法的研究胞中表達，結果發現雖然htr基因的rna量增加了，但白細胞的端粒酶活性並沒有發生明顯的改變，說明htr基因的過表達不能增強白細胞端粒酶活性。
  13. Experimental study on applying tgf - 1 gene transfection to modify in vivo tissue engineered cartilage

    兔骨髓幹細胞向軟骨細胞分化用於半月板組織工程重建
  14. 24, 48, 72 hours later after transfection, we testified the expression of pp38 with mab h19, and pp24 with the antiserurn of pp24 expressed in e. coli. the tests justified the expression of pp24 in prokaryotic and eukaryotic expressing systems. in order to study the correlation of pp38 and pp24, we cloned pp38 gene and pp24 gene into pbudce4

    為研究pp38和pp24之間的關系，將型mdvmd11株的pp38基因和pp24基因的完整orf克隆到真核雙表達載體pbudce4 . 1中，轉染cef后，通過ifa檢測和用抗pp24多克隆血清進行western - blotting試驗檢測到了pp38和pp24磷蛋白的共表達。
  15. In this study, a 1. 7kb kpni fragment and a lacz gene expression cassette carrying the e. coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ). the new transfer vector was called puni - lacz. the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure

    本研究以呈ge ~ -表型的經典弱毒疫苗bartha - k61株為親本株，在通用prv轉移載體pbdtk - uni的基礎上，在其多克隆位點中插入由sv40早期啟動子控制下的lacz基因表達盒，同時將下游同源臂增加了一個1 . 7kb的kpni片段，使上下游同源臂的長度都超過了1kb ，構建了一個新的轉移載體puni - lacz 。
  16. Transfection of the gp64 - mi and reintroducing hal33 gene bacmid dna into sf9 cells shown that hal33 can rescue the gp64 null bacmid in sf9 cells

    對該重組病毒bv的westernblot檢測，結果表明fi和fz同時存在並以二硫鍵相連。
  17. Construction of eukaryotic expressing vector of human epidermal growth factor gene and its gene transfection

    人表皮生長因子基因真核表達載體的構建及其基因轉染
  18. 5 in vitro specific killing experiments showed that spleen cells induced by pi, p3 and p4, which contained higher proportion of cdg + cells and generated better ctl activity, could be introduced to in vivo experiment against hcv 6 we successfully setup a hcv structural gene in vitro cell - transfection system sp2 / 0 - hcv, through which also setup a murine model with subcutaneous transplanting tumor of hcv

    將誘導的5種v特異性c l ，在體外進了丁特異性殺傷實驗，證一實，出n 、 p3和p4三條多肽誘導的脾糾泡含較高比例的cd 。糾1巾d ，叫產上較好的ctl殺傷活性，可以用作t細胞疫苗進fi體內抗hcv實驗。
  19. Furthermore, the egfp - eo gene was also cloned into pci - dhfr, a cho ( dhfr " ) cell expression vector. following the transfection and mtx selection, some cho cells presented green fluoresence, which will establish a good foundation for production of soluble eo glycoprotein and investigating its biological functions

    將p2種子液以moi5 - 10接種指數期sf9細胞，三天後呈現出最強的熒光。我們還將egfp - eo融合基因插入哺乳動物細胞表達載體pci - dhfr中，構建重組質粒pci - dhfr - egfpeo 。
  20. To confirm the approval of recombinant pcdnas - tgfjtf, gene and the results of its transfection, we used immuhistochemical staining ( sabc ). the department of or a logy 4 multiplication and differentiation of bmscs were observed by flow - cytometry ( tcm ), transilluminating electron microscope ( tem ) and other methods. the bmscs in controlled group was transfected with pcdna3 only

    採用電泳檢測pcdna3 - tgf _ 1構建是否成功；通過tgf _ 1免疫組化染色檢測轉染是否成功；運用形態學觀察、透射電鏡（ tem ） 、流式細胞儀（ fcm ） 、堿性磷酸酶（ alp ）染色、型膠原免疫組化染色等方法，觀察轉染后bmscs增殖與分化情況。
分享友人
分享到 Line

分享到臉書