gene transfer technique 中文意思是什麼

gene transfer technique 解釋
基因轉移技術
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • transfer : n 1 移轉，轉送；調職；調任[轉學]證書；變換。2 （財產；權利等的）轉讓，讓與（證書），移轉，授受；...
  • technique : n. 1. （專門）技術；（藝術上的）技巧，技能。2. 手法〈如畫法，演奏法等〉。3. 方法。
  1. Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique

    本研究的特色和創新之處在於，針對羅氏沼蝦不耐低溫，但體型相對較大，精莢明顯的特點，首次將目前已知具有最強抗凍活性的雲杉卷葉蛾（ sprucebudworm ， choristoneurafumiferana ）抗凍蛋白（ sbwafp ）基因（ sbwafp ） ，通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中，以期培育出耐低溫的羅氏沼蝦新品系。
  2. This system included method of solid medium plate enveloped with parafilm to form colony, conjugational gene transfer in a selective medium, screening the nonmagnetic mutants by magnet adsorption technique

    此體系包括：以平板封膜培養技術獲得單菌落，在選擇性培養液中進行遺傳因子接合轉移，以液體培養和磁鐵吸附技術篩選突變子。
  3. 3. using clamp technique, ex vivo gene transfer into liver graft was performed during cold preservation via perfusion of the portal vein with 5ml ringer ' s solution containing replication - defective adenovirus vector adhuctla4 - ig

    供肝冷保存時，採用血管夾技術lamptechnique ）經門靜脈灌注攜帶融合基因hllctla4dg的重組腺病毒，于術后3天、 7天能定性檢測到hllctla4ig在受體外劃血卜1 。
  4. Using ex vivo gene transfer technique, exogenous gene was introduced to the liver graft during cold preservation and express locally in the graft. the effect of inhibition of rejection and inducing liver graft tolerance was observed. through this study, the possibility of achieving graft tolerance by gene transfer without routine immunosuppressive drugs was explored

    我們採用adeasy腺病毒載體系統，自行構建攜帶huctla4 - ig的重組腺病毒，通過先體外后體內（ exvivo ）基因轉移技術，于供肝冷保存時，將該治療基因導入大鼠移植肝，使其于移植肝局部表達，觀察其抑制排斥反應，誘導大鼠移植肝免疫耐受的作用。
  5. Though the technique of nuclejc transformation in plants has been developed and used widely, some problems in genetic information have not been resolved. for example, because the nucleic genome is so big and complicated that the integration sites and copies of foreign gene can not be controlled accurately, the expression of transferred genes is inefficient as a result of gene silencing or position effect. in nucleic transformation, furthermore, the transfer of multigene is difficult, and only after the prokaryotic genes undergo modification are they expressed in high plants

    植物的細胞核轉化技術已發展成熟並得到廣泛應用，但核基因組的遺傳轉化仍存在一系列至今尚未解決的問題：例如由於核基因組大，背景復雜，外源基因的整合位點和整合的拷貝數難以人為控制，造成鄭州大學2003年博士學位論文杜氏鹽藻（ dunaliellasalina ）葉綠體轉化研究外源基因表達效率低，容易出現基因失活、基因沉默、位置效應等現象;同時轉入多個基因時操作步驟過于復雜，所表達的原核基因必須經過修飾改造，環境安全難以保證等。
  6. Establishment of conditionally immortalized rat cochlear greater epithelial ridge and lesser epithelial ridge cell lines using a retrovirus - mediated gene transfer technique

    大鼠耳蝸大上皮嵴及小上皮嵴細胞永生細胞系的建立
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