gene trapping 中文意思是什麼

gene trapping 解釋
基因捕獲技術
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • trapping : （油墨轉移力）疊印
  1. In the previous work, we cloned sh2a gene in this region by exon trapping and exon linking, which is a novel member of sh2 family. sh2 domain is considered as " protein recognized code "

    在前期工作中，運用外顯子捕獲和外顯子拼接等策略，我們在這一區域克隆了sh _ 2a基因，是sh _ 2信號蛋白家族的新成員。
  2. Normal tissues and tumor tissues methods a gene was cloned by exon trapping and exon linking technique. homologous analysis of the gene was researched by blast. then we explored the gene expression pattern in various human tissues by northern blot and rt - pcr

    正常組織及腫瘤組織實驗方法利用捕獲的外顯于進行blast同源性分析，之後採用外顯于拼接技術克隆新基因，利用rtpcr及northern印跡雜交檢測該基因的組織表達情況，同時以p 《 ctin作內對照。
  3. The exon trapping, an " cdna - indepentent " technology, has been used for several years. its virtue is to know the position of the exon, exclude the others sources and eliminate the problems of the putative positive or reselection. so, exon trapping is one of the most important means for isolating the disease gene at susceptible sites

    近年來採用的外顯子捕獲是一種「 cdna -非依賴性」的技術，這種方法的優點是得到的外顯子都已進行了定位，排除了其它來源的可能性，消除了「 cdna -依賴性」克隆技術的假陽性及復選性問題，因此它是從易感位點分離疾病基因的重要途徑之一。
  4. Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced

    在一步轉化系統中，光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求，並且簡化、優化了整個共培養過程，省去了一篩、二篩和預分化步驟，只用7周的時間就可以初步得到再生轉化植株；共191塊愈傷組織得到125塊抗性愈傷組織，轉化頻率達到65 . 4 ，最後共得到99棵獨立來源的再生植株，抗性愈傷組織再生頻率達到79 . 2 。
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