genes for expression 中文意思是什麼

genes for expression 解釋
表現基因
  • genes : 格涅斯
  • for : FOR f o r ＝ free on rail 【商業】火車上交貨（價格）。1 〈表示目標、去向〉向，往。 leave [sail] f...
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. These confirmed the successful transformation of the a78 - 3 and a78 - 4 on selection medium containing basta. microarray on membranes were fabricated from a set of 384 pinus taeda genes ( cdnas ) related to lignin synthesis, adaptation or primary metabolism for examination of gene expression in the sublines. the results showed : ( 1 ) the correlation coefficients between the transgenic sublines a78 - 3 and a78 - 4 and the untransformed control a95 : 88 : 22 were 0. 8028 - 0. 9028, while those of a78 - 5 are 0. 8897 - 0. 9302

    選擇384個與木質素生物合成及植物生理代謝和環境適應性有關的基因或cdna片段構建尼龍晶元膜，並對轉基因細胞亞系a78 - 3 、 a78 - 4和a78 - 5和對照亞系a95 ： 88 ： 22等培養再生植株進行基因表達的微陣列檢測，結果表明： （ 1 ）三個亞系與對照之間的pearson相關系數分別為0 . 8607 、 0 . 7975和0 . 9630 。
  2. Fusion protein expression system can overcome those problem, increased the yield of yield of recombinant protein in e. coli. this remarkable increase in protein yield was thought to be due to protection of the target protein from proteolysis, improved folding, and efficient mrna translation. fusion protein also make the detection and purification easy, is a good strategies for achieving high - level expression of genes in escherichia coli

    小分子量異源蛋自在人腸桿菌的表達受mrna不穩定、翻譯起始效率低、易被蛋自酶降解等因素的干擾，較難獲得高效表達，通過與已高表達的蛋自融合表達可以克服以上問題，可以使大多數蛋白獲得高效表達。
  3. 13 chatterjee - kishore m, wright k l, ting j p, stark g r. how stat1 mediates constitutive gene expression : a complex of unphosphorylated stat1 and irf1 supports transcription of the lmp2 gene. embo j., 2000, 19 : 4111 - 4122. 14 mahboubi k, pober j s. activation of signal transducer and activator of transcription 1 stat1 is not sufficient for the induction of stat1 - dependent genes in endothelial cells

    例如， jak - stat中， socs1 ppn以及stat1蛋白對于系統輸出的影響相對其他蛋白來說要明顯的多，這三個蛋白主要控制著jak - stat信號通路的「信號幅度」和響應強度response magnitude ，最大響應值。
  4. Recombinant fpvs were selected and purified by blue plaques expressing 3 - galactosidase. the expression of foreign genes in rfpvs were confirmed by ifa. trails for evaluating protective efficacy of rfpvs against very virulent mdv or aiv challenge

    純化后脂質體轉染，藍斑篩選純化得到穩定的重組病毒rfpv - ps - ha - pe / l - f ，間接免疫熒光法證實， ha基因和f基因同時得到了表達。
  5. The dna microarray is capable of profiling the expression levels of many genes simultaneously, and is a promising technology for the elucidation of gene interactions. and how to extract the regulatory information hidden in the millions of data points that result from the microarray experiments has become a problem that researchers are eager to resolve

    基因晶元（ genechip ， microarray ）作為一種高通量的檢測方法，可以同時測量成千上萬個基因的表達水平，已成為后基因組時代研究基因間相互作用的一個有力的工具，如何從晶元實驗產生的表達數據中揭示出其所蘊含的豐富的調控信息是研究者都渴望解決的問題。
  6. When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a, 109 candidates interacting with nifa had been selected by testing for the expression of the his3, ade2 and lacz reporter genes

    誘餌質粒和文庫質粒共轉化釀酒酵母（ saccharomycescerevisiae ） pj69 - 4a ，通過檢測報告基因his3 、 ade2及lacz的表達進行篩選，初篩得到109個陽性酵母菌落。
  7. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  8. To further confirm the expression levels of those differentially expressed genes in rat brain of + gz exposure group, the primers for two known genes ( 53 # and 72 # ) and one unknown genes ( 58 # ) were designed for semi - quantitative reverse transcription polymerase chain reation ( rt - pcr ), using gapdh as the reference

    4 、進一步驗證了通過ssh方法得到的差異表達基因片段在+ 1ogz重復暴露腦組織中的差異表達情況。對兩個己知基因克隆53 # 、 72 #及一個新基因克隆58 #分別設計引物，以gapdh為內參照，進行半定量rl 、 pcr反應。
  9. One dataset available for such an analysis involved gene expression profiling of the early growth factor response to platelet derived growth factor pdgf in a human glioblastoma cell line ; this study differentiated genes whose expression was regulated by signaling through the phosphoinositide - 3 - kinase pi3k versus the extracellular - signal regulated kinase pathways

    本文採用人膠質母細胞瘤細胞對血小板衍生生長因子的早期反應特徵性基因表達數據，以區分通過磷酸肌醇- 3 -激酶和細胞外信號調控激酶信號途徑調控表達的基因
  10. Then, nap300 and 75 were fused in a similar way to phba gene encoding 3 - ketothiolase for a direct comparison. the result indicated that the two promoter ' s expression efficiency reached a peak at the same developmental stage of tobacco, which means they have the advantage of being used simultaneously for expressing different foreign genes in plant

    將nap300 、 7s分別與phba基因（編碼3 -酮硫裂解酶）相連，在相似表達環境中對二者功能進行比較，發現兩個啟動子表達模式基本相同並在同一時期達到活性高峰，因此nap300可用於改善phb合成基因在植物體內的表達調控。
  11. With the advance of human genome project, bioinformatics has been promptly developed in recent 20 years. as a branch of a new class of bioinformatics which allows the monitoring of expression levels for thousands of genes and proteins simultaneously, biochip technologies are increasingly applied to broader fields

    隨著人類基因組計劃的提出與開展，生物信息學在近20年內得到了迅猛的發展，作為生物信息學領域一個新分支的生物晶元技術由於能夠同時對成千上萬的基因和蛋白質片斷進行表達分析，已經得到了越來越廣泛的應用。
  12. Discussion in the process of development, differentiation and metabolism, the differential expression of genes plays a pivotal role. mrna differential display, established by liang and pardee in 1992, is among the most sensitive methods for the isolation of differentially regulated mrna

    經rt一pcr反應篩查17例脊索瘤及其周圍正常組織，擴增片段大小與引物設計一致，可見瘤周組織rnf一n基因表達明顯增高，而ctc一sodms在腫瘤組織中表達增高。
  13. For gene expression similarity analysis, we find that there tends to be higher correlations among co - regulated genes when transcription - rate - based correlations are used compared to those based on transcript amounts

    我們運用了cho等人的酵母細胞周期mrna量以及wang等人的酵母mrna降解率。在基因相似性研究中，通過數據庫， young等人的chipchip數據，我們可以得到共調控基因。
  14. In contrast, there was very little difference in gene expression between calli subcultured for one week and two weeks with only 0. 78 % of the genes showing significant different expression. ( 4 ) the 14 genes with different expression level between the control and the transformed sublines a78 - 3 and a78 - 4 were classified by their function

    88的未鑒定功能基因的表達呈顯著差異；而在愈傷組織培養二周與愈傷組織培養一周之間，則在第大類、第v大類和第大類未鑒定基因中各有1個表達呈顯著差異。
  15. Experimental protocol were performed by instruction manual of lightcycler - rna amplification kit sybr green i. human named genes hgec - 40s were used for differential expression screening

    以未作任何處理的內皮細胞作為對照。內皮細胞切應力加載完成後，提取細胞總rna 。
  16. A corollary has been that proteins, in addition to their structural and enzymatic roles in cells, must be the primary agents for regulating the expression, or activation, of genes

    在這個推論下，蛋白質除擔負細胞結構和酵素的角色外，必定也是調控（或活化）基因表現的主要因子。
  17. Abstract : the low expression level of foreign genes in transgenic plants is a puzzling question for researchers in plant genetic engineering. the biosafety concern is now causing a growing public wariness of transgenic plants around the world. this article reviews the new strategies currently used in construction of plant expression vector for plant genetic engineering. these strategies are important for obtaining better expression of foreign genes and developing safer transgenic plants

    摘要外源基因在轉基因植物中表達效率低一直是令相關研究者困擾的一個問題.轉基因植物的生物安全性最近在全球范圍內開始引起世人的擔憂.本文簡要介紹了近年來在植物表達載體構建方面所採用的一些新策略，這些策略有助於增強外源基因的表達水平、提高生物工程體的安全性。
  18. Introduced infected by the turnip mosaic virus. there are many useful objective genes can be used in vegetable genetic transformation, but the research work of chinese cabbage genetic transformation is little. the reaserch on transforming chinese cabbage using agrobacterium - med ated method has n ' t been report at home and abroad. ln the test, tumv - cp gene was transferred into chinese cabbage ( brassica campestris l. ssp. pekinensis ) via agrobacterium - mediated method. a high effective regeneration and genetic transformatin system has been established, the detection by the method of molecular biology, has proved that the regenerative plants are transgenic plants and the target genes have been expressed transgenic plants partly. meanwhile transgenic progenies were traced and investigated so that heredity, stability and expression of target gene were researched. the virus resistant, stable plants were expected to obt ain so that theoretical base can be established for chinese cabbage breeding by gene engineering

    利用農桿菌介導法轉化大白菜抗病基因的研究工作在國內外未見報道。本課試驗採用農桿菌介導法將tumv - cp基因導入大白菜中，建立了高效的大白菜離體再生、遺傳轉化體系，並對轉基因植株進行分子生物學檢測，證實得到的再生植株為轉基因植株，目的基因已在部分植株上表達。同時，對轉基因植株的後代進行檢測，分析該基因所控制性狀的遺傳穩定性以及基因表達情況，為大白菜基因工程抗病育種提供理論依據。
  19. A further analysis using human embryonic tissues ( 16 to 24 weeks ) showed a development - specific expression pattern in heart, skeletal muscle, liver, lung, kidney and brain suggesting a role for these genes in embryonic development

    胚胎組織（ 16周- 24周）的northern雜交結果表明隨著發育階段的不同，以上四個基因在心臟，骨骼肌，肝，肺，腎和大腦中的表達量有一定的變化，這說明它們在胚胎發育過程中起著一定的作用。
  20. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
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