genomic library 中文意思是什麼
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In summary, we constructed and characterized a bac library of silky, a unique chinese native breed of chicken. the library has high genome coverage ( 13 - fold ), chimerism ( 6 % ) and overlapped bacs, which made it a valuable resource to complete chicken physical map, study functional genes and construct bac contigs. we analyzed the whole genomic sequence and snps of tyrp1 and found that silky tyrp1 is different with other breeds both in microsatellite and transcription regulation site
中國農業人學博卜學位論文摘要本研究構建了中國特有雞種絲羽鳥骨雞基因組bac文庫並進行了文庫質量鑒定，它所具有的13倍高基因組覆蓋率、 6 %的嵌合率和部分重疊的克隆使其成為完善雞的基因組圖譜、研究基因功能和構建bac重疊群的優質資源。 -
When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a, 109 candidates interacting with nifa had been selected by testing for the expression of the his3, ade2 and lacz reporter genes
誘餌質粒和文庫質粒共轉化釀酒酵母（ saccharomycescerevisiae ） pj69 - 4a ，通過檢測報告基因his3 、 ade2及lacz的表達進行篩選，初篩得到109個陽性酵母菌落。 -
Then pseudomonas sp genomic library was constructed
陽性克隆的篩選工作仍在進行中。 -
In this paper, we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid
以dig - oligo2為探針，菌落原位雜交篩選cosmid文庫，得到56個陽性重組子。 -
Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence
以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。 -
Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis. so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library. cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids
以此片段為探針釣出了位於該區域的文庫克隆，並利用指紋印跡和雜交技術將這些文庫克隆排列起來，進而以位於該區域的不同位置的片段做臂構建了用於轉化和接合轉移用的基因置換質粒，並試圖通過基因置換將該區域置換下來，但尚未得到最終結果。 -
In this paper several methods of developing microsatellite primers in plants were summarized : to search the database and find the primers which were delivered ; to transfer them among the different species ; to establish genomic library ; to develop ssr primer based on moleculars markers
摘要綜述了開發植物微衛星引物的幾種方法：搜尋核酸數據庫和尋找已存在的微衛星引物；不同物種間共用；建立基因組文庫；利用分子標記技術發展ssr引物。 -
The library consisted of 1. 3 x 106 clones with an average insert size of about 18kb. the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin. screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr, we got four positive clones
3x10 『個重組噬菌體，插入片段大小約為18kb ，含插入片段的頻率為100隊以中亞濱蓉甜菜堿醛脫氫酶門adh ）基因近5 』端的約400hp片段為探針，篩選中亞濱蓉基因組文庫，得到了4個陽性克隆。 -
It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0. 5 - 3kb fragments of a. brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors
Brasilensesp7的nifa全序列構建在pgbd - c2載體上，得到重組質粒pgbd - nifa ，以其為誘餌，篩選sp7質粒基因組文庫（構建在pgad系列質粒上） 。 -
In the past, we cloned the cdna of zmcdc5 based on the studies on difference of gene expression in shoot and radicle of maize seedling in our lab and then got the whole genome sequence by maize genomic library screening and inverse pcr
本實驗室根據玉米胚芽和胚根差異表達的研究，克隆得到zmcdc5基因的cdna ，並通過篩庫和反向pcr獲得了zmcdc5基因組全長序列。 -
Its genomic dna was partially digested by sauial. dna fragments from 4 to 16 kb were collected after electrophoresis and ligated with bamhi - digested puc18 to construct genomic library. the total number of recombinant plasmids is about 9000
進一步在opua基因的上下游序列分別設計引物，從h . trueperi基因組dna中擴增出所預期大小的片段，測序驗證已獲得opua基因的全序列。 -
The work on physical mapping of the chromosome of s. nanchangensis ns3226 was initiated. nearly a full set of chromosomal asei - bamhi fragments of s. nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library. thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion, suggesting distinct identifications
此外，還開展了南昌鏈黴菌ns3226染色體物理圖譜構建的前期研究工作：基本克隆到了南昌鏈黴菌ns3226染色體上全套的ase - bamh片段，以它們為探針從南昌鏈黴菌ns3226的基因文庫中釣到164個陽性克隆，並從中篩選到34個ase linkingcosmids ，用bamh進行初步的酶譜分析，結果表明其中有20個cosmids的bamh酶譜相互間沒有明顯的重疊性。 -
A genomic dna library of atriplex centralasiatica iljin was constructed using lambda embl3 / bamh i as vector
為了得到badh基因的上游序列，我們以lambdaembl3 budl為載體，構建了鹽生植物中亞濱茵的基因組文庫。 -
Previously, we have constructed a 400mmol / l nacl - treated cdna library of s. salsa and acquired 1000 ests ( expressed sequence tags ) by the large - scale partial sequencing of randomly selected cdna clones. in this research, we isolated a cdna that maybe encode an aquaporin ( s. salsa plasma membrane intrinsic protein, sspip, bf023789 ) located in plasma membrane of s. salsa. then we analyzed the sequence characterizations, genomic structures and transcriptional levels under salinity stress
本實驗室構建了400mmol lnacl處理的鹽地堿蓬（ s . salsa ）地上組織的cdna文庫，隨機挑取單克隆進行測序，獲得了可能編碼鹽地堿蓬質膜水通道蛋白（ s . salsaplasmamembraneintrinsicprotein ， sspip ）的cdna ，其序列號為bf023789 。 -
The me2155 genomic library was constructed using phz132 as the cosmid vector, and 10 positive clones were fished out using 0. 9kb pcr product as probe
以phz132為載體，構建了mc ~ 2155的基因組文庫。以0 . 9kb的擴增產物為探針從文庫中釣出10個陽性克隆子。 -
Genoraic dna of b. megaterium was partially digested by restriction enzyme sau3a and was used to establish the genomic library in plasmid pbluscripts. selection for endoglucanase positive clones from transformed e. coli was carried out on the cmc medium. seventy - four clones that showed hydrolysis ability on the cmc plate were obtained
用ecori和sall對含有幾丁質酶基因的重組質粒tvchi （含幾丁質酶編碼基因的pmd18一t載體）和穿梭質粒phy300plk進行雙酶切並連接，轉化e . colidhs 。 -
In the first part of this experiment, we isolated the tsmt gene ( genebank accession number : af499726. 1 ) from a a zap - cdna library cons tructed from a 200mmoll - 1 nacl - treated library of thellungiella salsuginea and analyzed its sequence characterization, genomic organization and the differential expression in response to salt stress. tsmt encodes metallothionein and there is only one copy in thellungiella salsuginea genome according to its southern blotting
L ~ （ - 1 ） nacl處理的鹽芥地上部分構建的zap - cdna文庫中克隆了編碼金屬硫蛋白的基因tsmt （ genebank號為af499726 . 1 ） ，並分別對其序列特徵、基因組結構和在鹽脅迫下的表達特性進行了分析，結果表明tsmt編碼鹽芥金屬硫蛋白， southern結果顯示tsmt在鹽芥基因組中只有一個拷貝。 -
Screening of a potato genomic library with a full - length sb401 cdna resulted in the isolation of six positive clones. one of clones, designated 901, was further characterized
對花粉發育過程中特異表達基因的研究，科學家們希望從分子水平上闡明小孢子發育的本質。 -
Through in situ hybridization and colony pcr, a positive recombinant plasmid was isolated from the genomic library and sequenced. the inserted dna fragment was about 4. 3 kb
將pcr擴增片段用地高辛標記探針，利用原位雜交和菌落pcr從基因文庫中獲得含有陽性信號的重組質粒。
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