genomic walking 中文意思是什麼
genomic walking
解釋
基因組步查,基因組步移-
Using random primed gene walking pcr ( rpgw - pcr ), ctsox2 gene was cloned and sequenced from the digested genomic dna of trionyx sinensis. compared with the mouse and chicken sox2, ctsox2 shared 86 % and 91 % nucleotide homology respectively, and 93 % and 97 % amino acid identity respectively
天i消化過的中華鱉（ trio矛移優sinensis ）基因組dna中克隆了c眨治xz基因（ 72obp ） ，它與鼠、鳥的及戲2基因分別有85 %和91 %的核昔酸同源性， 93 %和97 %的氨基酸同源性。 -
And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。
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