gfp 中文意思是什麼

gfp 解釋
玻璃纖維增強塑料
  1. The changes of the expression of gfp in biu - 87 cell that induced by the aconitine and hab toxins, gtx were detected with fluorescence microscope, and quantitatively measured with image - pro plus software

    經誘導，抗性細胞發出較強的綠色熒光，表明重組質粒pegfp - c - fos在biu - 87細胞中成功表達。
  2. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  3. By using the gfp - labeling technique, we have found that gfp - cam colocalized with all the characterized structures formed during cytokinesis

    通過採用gfp標記技術，我們觀察到gfp - cam與胞質分裂期細胞內形成的特徵結構存在共分佈。
  4. To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus

    在構建了紅色熒光蛋白aes表達載體后，將其與tle綠色熒尤蛋白載體共轉染細胞，共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象，而且aes的表達可抑制tlei向胞核內的聚積。
  5. By tagging the oxt gene with a gfp tag, it was found that this gene mainly expressed in the cytosol

    通過把。 xt基因加上gfp標記，發現該基因主要在細胞質中表達。
  6. Using gfp retrovirus to label tumor cells and vascular endothelia cells

    逆轉錄病毒標記腫瘤與血管內皮細胞
  7. The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments. based on this, the co - transfection systems used in the study was established. we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342, after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc

    當分別共轉染附加kozac序列和核定位信號的gfp與人集縮素smc亞基hcap一e特異的rnai質粒rhe和人集縮素smc亞基hca屍一c的f { nai質粒rhc時，都觀察到gfp和hochest33342標記的轉染后hela細胞表現多核和染色質橋現象。
  8. Polymer his - tagged peac1 - gfp efficiently activated myosin mg - atpase activity, which indicated that peacl might take part in correlative living activities in vivo. moreover, this result provided experimental proof in vitro for fusing gfp to actin isoform directly to study the dynamics of microfilaments and its regulation in vivo. we prepared rabbit anti - pea actins polyclonal antibodies using peacl as antigen which being expressed and purified from prokaryotic cells, and the antibodies possessed better immunity activity to pea actins

    通過肌動蛋白體外對dnase以及肌球蛋白atpase活性影響的研究，發現單體his - taggedpeac1 - gfp能顯著抑制dnase活性，在肌動蛋白聚合條件下能有效激活肌球蛋白atpase活性，這一結果預示著peac1在體內可能參與相關的生命活動，為利用gfp直接與肌動蛋白異型體融合來研究體內微絲的動態變化及其調節提供了實驗依據。
  9. Gfp - oscam61 was transported into the nucleoplasm upon a block in isoprenoid biosynthesis by mevinolin treatment of tobacco cells. these results indicate that the prenylated oscam61 molecules are mainly membrane - associated while its unprenylated counterparts are transported into the nucleoplasm. thus, oscam61 may play functions in coordinating ca2 + signaling with isoprenoid metabolism

    用抑制異戊烯合成途徑的mevinolin處理轉化了gfp - oscam61的煙草細胞，原來定位於膜上的gfp - oscam61則進入細胞核，說明異戊烯化的oscam61結合在膜上而它的非異戊烯化形式存在於細胞核質中，因此， oscam61同時受鈣信號和異戊烯代謝的調控，並可能在鈣信號傳遞和異戊烯代謝的協調過程中發揮功能。
  10. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  11. We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously. there are three kinds of combination probability that could be observed by gfp marker

    在實驗中同時共轉染人集縮素smc亞基hcap一e和hcap一c特異的rnai質粒rhe和rhc和pcdna3 . 1 + kg到hela細胞。
  12. They are combination of co - transfection with rhe and gfp expressing plasmids, co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time. whichever the combination may be, we always observed the phenomena of chromatin bridge in co - transfected cells. these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits

    這時，存在三種可能的情況，即共轉染rhe和pedna3 . 1 * kc 、共轉染rhc和pedna3 . 1 + kg 、共轉染rhe和rhc和pc洲a3 . 1 + kg ，但都觀察到明顯的染色質橋現象，進一步證明這種表型是人集縮素中smc亞基的缺陷的特徵表型。
  13. Root nodules of the soybean and alfalfa inoculated with xj96077 ( g ) and colony of xj96077 ( g ) were observed using the confocal laser scanning microscope, and the expressions of gfp were detected

    而且，通過不同品種的苜蓿、大豆的結瘤試驗，發現xj96077對不同大豆品種的結瘤能力不同。
  14. Sv40 polya signal and two loxp sites was added consecutively after the termination codon taa, and then a gfp gene was inserted between the two loxp sites

    在三個終止密碼子taa之後，連上sv40polya 。 sv40polya之後，又連上兩個loxp位點。在兩個loxp位點，插入標記基因gfp 。
  15. High stability of ptr102 - derived marking plasmids indicated that novel vector systems based on ptr102 could be constructed for the study on molecular biology and ecology of m. huakuii. 1kb gfp cdna fragment amplified by pcr was cloned into e. coli expression plasmid pet - hc, which was under the control of rna polymerase gene promoter from phage t7 with its own translation initiation codon

    將pcr擴增的1kbgfpcdna片段克隆到大腸桿菌表達載體pet - 11c上，使gfpcdna在帶有lac操縱基因的t7噬菌體rna聚合酶基因啟動子的控制下、以自身的atg作為翻譯起始密碼進行翻譯。
  16. In this study, in order to examine the dynamics of tip [ ca2 + ] i together with the dynamics of tip - localized f - actin in vivo, a kind of double labeled material was constructed. the ca2 + and actin microfilaments of arabidopsis pollen tubes were labeled by cameleon and gfp - mtalin respectively

    本研究以擬南芥花粉管為材料，通過轉基因技術，將分別標記ca ~ （ 2 + ）和微絲的兩種熒光蛋白cameleon與gfp - mtalin在花粉管中同時表達，實現活體花粉管中ca ~ （ 2 + ）與微絲的同時標記。
  17. The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter, length and shape being identical to that of muscle f - actins, which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ), and were identified as having a 9 nm diameter by negative staining, corresponding with that of the muscle f - actins ( 7 - 10 nm ). under polymerization conditions, his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin, that is, an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained. the critical concentration is 0. 75 umol / l near to that of chicken muscle actin ( 0. 56 umol / l ) under the same condition

    熒光標記結合熒光顯微觀察表明：從可溶性上清中純化的his - taggedpeac1 - gfp聚合形成的微絲不僅可以直接在熒光顯微鏡下觀察，也可被微絲的特異標記物鬼筆環肽所標記，而且其直徑、長度以及形態上與已知的聚合肌動蛋白熒光絲一致；電鏡負染的結果進一步證實其直徑為9nm ，與傳統微絲直徑相當（ 7 ? 10nm ） ；聚合曲線有明顯的停滯期，為典型的s型聚合曲線，聚合臨界濃度為0 . 75 mol l ，這一結果與已有報道相似。
  18. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  19. We successfully construct the eukaryotic expression vector of gfp - eif - 5a and its mutational vector using genetic engineering techniques. we found that eef - 5 a localized in nucleus as well as in cytoplasm just for a short time after its transient expression, then distributed only in cytoplasm

    Eif - 5a的hypusine修飾是其活性和功能發揮所必需的，我們通過pcr方法實現了hypusine位點的定點突變，並進一步構建了含gfp標簽的eif - 5a及其hypusine位點突變的真核表達載體。
  20. Furtherly expression phase analysis showed that orf2 appeared in different form during infection. to reveal the function of haorf2, vha - orf2 - gfp recombinant virus which tagged with egfp - orf2 fusion protein was generated

    構建了ha2與綠色熒光蛋白融合表達的重組病毒vha - orf2 - gfp ，對該重組病毒感染細胞后，融合篡碩士學位論文mast卜r 』 s 』 r拼ifs
分享友人
分享到 Line

分享到臉書