gvbd 中文意思是什麼

Protein kinase a is camp - dependent kinase, one of the most important signal transduction pathways, plays a pivotal role in growth, differentiation, tumor occur, cell cycle control, etc. pka activators such as camp, 8 - br - camp or phosphodiesterase ( pde ) inhibitor isomethyl butyl xanthine ( imbx ) or purified pka catalytic subunit all can inhibit germinal vesicle breakdown ( gvbd ) and meiotic maturation in mouse oocytes, also in xenopus oocytes

蛋白激酶a （ proteinkinasea ， pka ）是依賴于camp的絲蘇氨酸蛋白激酶，是重要的信號傳導途徑之一，廣泛參與許多生命過程，包括生長、分化、腫瘤發生、細胞凋亡、細胞周期調控等。以小鼠卵母細胞為實驗對象，給予pka激動劑camp 、 8 - br - camp或磷酸二酯酶抑制劑imbx （ isomethylbutylxanthine ）均可抑制小鼠卵母細胞的胚泡破裂及減數分裂成熟。

Rat cumulus - enclosed oocyte ( ceo ) and denuded oocyte ( do ) both spontaneously resume meiotic maturation in vitro, when cultured for 2h, the germinal vesical breakdown percentage of do reaches up to 88 %, and ceos get 53 %. oocytes begin to extrude first polar body 1 ( fb1 ) when cultured for 8h. mouse oocyte spontaneous maturation in vitro can be inhibited by 4mm hypoxanthine ( hx ) or 25um 3 - isobutyl - methylxanthine ( ibmx ). while, 4mm hx can not inhibit the spontaneous maturation of rat oocyte, and the lowest effective concentration of ibmx to inhibit rat oocyle spontaneous maturation in vitro is 50um. treated with the same concentration of ibmx, gvbd percentage of rat ceo is lower than that of do, the results indicat that cumulus cells delay rat oocyte spontaneous maturation and potentate the inhibition effect of ibmx, but it ' s not the case on mouse

Ceo和do培養8h時都開始排出pb1 ；在卵母細胞成熟抑制模型中， 4mm次黃嘌呤（ hx ）和25 m的異丁基甲基黃嘌呤（ ibmx ）是小鼠卵母細胞成熟的有效抑制劑，但4mm的hx抑制大鼠卵母細胞成熟效果不理想，用hx - m199和hx - mem培養24h ， gvbd率分別為61 ， 81 ，而ibmx有效抑制大鼠卵母細胞自發成熟的最小濃度為50 m ， ibmx可以降低大鼠ceo和do的gvbd率，但ceo中的卵母細胞gvbd率要顯著低於do的gvbd率， 100 m的jbmx處理24h后，大鼠ceo和do的gvbd率分別為24 ， 84 ，提示卵丘細胞的存在可以延遲大鼠卵母細胞的自發成熟，有助於抑制卵母細胞成熟，但在小鼠上ceo和do的自發成熟差異不明顯。

The calcium oscillation and distribution before gvbd in mouse oocyte

小鼠卵母細胞生發泡破裂前的鈣離子波動及分佈

By nuclear transferee transfered the nih 3t3 cell to enucleated gv and mii oocyte. the immunofluorescence results showed that jak. 2 was not transfered to somatoblast nuclear whether the recombined oocyte occured gvbd or not. when transfered somatoblast nuclear to enucleated and integrated m ii oocyte, the immunofluorescence results showed that jak2 only existed in the nuclear of integrated m ii oocyte but not the somatoblast nuclear. the nuclear transfer implied that the mature and immature oocyte cytoplasm could not transfer the jak. 2 protein to donor nuclear

通過核移植，將nih3t3體細胞核移入去核gv期和m期的卵母細胞中。熒光檢測發現，體細胞核與去核gv期卵母細胞質構成的重組卵無論是否發生gvbd ，核內均沒有jak2的出現。在去核和未去核的m期卵母細胞中移入體細胞核， jak2僅存在於未去核m期卵母細胞的核內，並不轉移到體細胞核內。