ha virus 中文意思是什麼

ha virus 解釋
virus]血細胞吸附病毒
  • ha : int. 哈!〈表示驚愕、快樂、疑惑、躊躇等〉。vi. 「哈!」地叫一聲。n. 「哈!」的一聲。
  • virus : n 1 【醫學】病毒；濾過性病原體。2 毒素；毒害。3 惡意，惡毒。4 【計算機】（電腦）病毒〈指擾亂或破...
  1. Sequence comparison indicated that the nucleotide homologies between the ha gene and those from eight other h3 subtype sfv isolates from china are over 95 %, and nucleotide identities between h3 sfv isolates in china and a typical h3 subtype avian influenza virus ( aiv ) strain dkuk1 - 63 are 95 % to 100 %. these might tell us that the ha gene of all h3 subtype sives isolated in china was originally from aiv although the vaccine based on ha do not protect against different subtype of influenza virus, it can provide favorable resistance to infection with antigenic variants within a subtype

    通過與其它7株國內siv分離株和6株genebank中的h3流感病毒ha序列比較分析，結果表明分離自我國不同地區的8株h3siv核苷酸序列同源性均在95 %以上，且與禽流感病毒（ aiv ）經典代表株dkuk - 63序列同源性在95 - 100 %之間，證實我國h3sivha基因來源於禽的流感病毒。
  2. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  3. Hepatitis a antibody ( anti ha ) - main laboratory test used to detect hepatitis a. detects antibody produced by patient ' s immune system in response to hepatitis a virus

    肝靜脈? ?肝臟主要的流出靜脈，連接肝臟和腔靜脈，然後到心臟。位置在肝臟上方。
  4. The purpose of this study is to construct a recombinant pseudorabies virus expressing ha gene of swine influenza virus and to develop specific diagnosis for differentiating the recombinant virus immunized animals and those naturally infected with prv or sfv

    本試驗的目的是以ge基因缺失的prv為載體構建可以表達sivha基因的重組病毒疫苗用於預防這兩種病，同時，基於此重組病毒的特點，建立可以區分此重組病毒疫苗和prv及siv自然感染的鑒別診斷方法。
  5. Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque

    利用脂質體介導的方法，將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞，依據報告基因lacz在細胞中的表達，篩選藍色蝕斑的重組病毒，經數次蝕斑純化后， pcr鑒定、 western - blot分析。
  6. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  7. The expression of ha in vero cells infected with rprv - ha was detected by western - blot. the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha. the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza

    結果表明所獲得的重組病毒（ rprvha ）遺傳性狀穩定，在培養細胞中能穩定的表達與sivha具有相似生物學活性的外源蛋白，為進一步制備抗豬流感的重組偽狂犬病毒活載體疫苗奠定了基礎。
  8. The hemaglutinin ( ha ) of aiv plays the key role in determing the pathogenicity, cell receptor binding property and host range of the virus. the homology of the ha sequences reported and registered in genbank of different strains of h _ ( 5 ). h _ ( 9 ) subtype was respectively analyzed and compared with each other. the conservative domin of ha seqence of h _ ( 5 ), h _ ( 9 ) subtype was selected for pcr amplification. two sets of specific primers were designed

    本研究根據genbank中查出的禽流感病毒h _ 5亞型，禽流感病毒h _ 9亞型的ha片段基因組序列，利用dnasis軟體分別對aiv的h _ 5 、 h _ 9亞型ha基因區域進行同源性比較，設計篩選出兩對聯合pcr反應的特異性引物，其中一對是h _ 9亞型通用引物，擴增出ha片段695bp ，另一對引物為h _ 5亞型的通用引物，擴增出的ha區域部分目的片段約為448bp 。
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