helix-ii 中文意思是什麼
helix-ii
解釋
膠原螺旋肽-
Abstract : the effects of terbium ion on the conformation of calmodulin and on the interaction between calmodulin and melittin have been studied by the endogenous fluorescent spectrometry of calmodulin and melittin, and the sensitized fluorescent spectrometry of terbium ion, respectively. the results show that terbium ions have a tight binding site in the i and ii metal - binding sites of calmodulin. the conformation of calmodulin induced by terbium ion can bind melittin and transfer the tryptophane residue of melittin to a relatively hydrophobic environment, while the binding of melittin to calmodulin produces effect on the binding orders of terbium ion in camodulin. results from ft - ir spectrometry have revealed that upon binding of lanthanum ion, apo - calmodulin undergoes a conformational change with the increase of - helix content and the decrease of - sheet content. melittin ' s binding to calmodulin has no effect on its conformation induced by the binding of lanthanum ion to calmodulin
文摘:分別用鈣調蛋白和蜂毒素的內源熒光光譜以及鋱離子的敏化熒光光譜考察了鋱離子對鈣調蛋白構象變化以及對鈣調蛋白與蜂毒素相互作用的影響.結果表明，鋱離子首先結合在鈣調蛋白的第和第位點，鋱離子不影響鈣調蛋白與蜂毒素的相互作用，蜂毒素與鈣調蛋白作用后不影響鋱離子在鈣調蛋白上的鍵合順序.傅里葉變換紅外光譜結果表明三價的鑭離子與鈣調蛋白作用使鈣調蛋白的螺旋結構增加，折疊結構減少，與鈣離子對它的二級結構影響相類似.稀土離子在鈣調蛋白-蜂毒素復合體系中主要與鈣調蛋白作用 -
Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
分享友人