high-molecular-weight protein 中文意思是什麼
high-molecular-weight protein
解釋
高分子量蛋白-
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。 -
It was found that prophenoloxidase ( propo ), isolated as a monomeric protein, had a molecular weight of 87. 5 kda, and a 77 kda phenoloxidase molecule was often contained in preparations. the 87. 5 kda propo had a very low enzymatic activity on 0. 02 mol / l l - dopa, whereas the 77 kda po had a very high enzymatic activity on it
實驗發現，酚氧化酶原（ prophenoloxidase ）的分子量為87 . 5kda左右，在實驗操作過程中極易發生降解或自身降解，變成有活性的大小約77kda的酚氧化酶， 1 sds可使該酶原全部被激活而成為有活性的酚氧化酶。 -
When the antigen ( brief named ag ) invaded into the organism, and the body can produce a kind of material ( high molecular weight protein, named antibody, brief named ab ) that can distinguish this antigen and evacuate it, the antigen and antibody take place the immuno - reaction. using their highly sensitivity to response mass change and specificity, it can be fabricated a piezoelectric immunnosensors
當異種蛋白（稱為抗原， antigen ，簡稱ag ）侵入生物體內時，體內能產生識別此類異物並將其排出的物質（大分子量蛋白質，稱抗體antibody ，簡稱ab ） ，抗原與抗體發生免疫反應，利用抗體（或抗原）對抗原（或抗體）的特異性識別功能和壓電晶體的高靈敏質量響應可製成壓電免疫傳感器。 -
Manufacturing protein drugs and high molecular weight biological by applying transgenic goat technology
應用基因轉殖羊技術生產蛋白質藥物和生物高分子 -
Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage
構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。
分享友人