homologous recombination 中文意思是什麼

homologous recombination 解釋
同源重組,同源重組合
  • homologous : adj. 1. 同源的。2. 【生物學】異體同型的。3. 【化學】同系列的；同屬列的；同周期的。4. 【醫學】同源的。5. 【醫學】＝ homoplastic.
  • recombination : 沖調；復水
  1. Homologous recombination in meiosis

    減數分裂中的同源重組
  2. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  3. Thirdly, the hyl gene was cloned to puc19 and pbr322 respectively. then the fragment containing ampr derived from puc19 and hasa came from pse380 - has were inserted into hyl either or both in vitro. transformed the recombinated vectors into s. equi by electro - operation, then plating on hag solid medium containing ampicillin, and selected clones hi which the hyl gene was inactivated by gene replacement through homologous recombination

    （ 1 ）直接插入外源基因滅活hyl方法：將hyl片段與載體puc19 pbr322連接后，用來自puc19的amp ~ r抗性基因片段從hyl基因的中部插入將載體上的hyl片段分為兩部分； （ 2 ）用hasa替換hyl部分片段的方法：將hyl片段與載體pucl9 pbr322連接后，用hasa片段替換部分hyl ，再將amp ~ r片段接入到hasa上游。
  4. The exchange of homologous regions between two dna moleculs. also called general recombination

    Dna分子之間同源區段的交換，也稱為一般重組。
  5. A deletion recombinant virus ( hasnpva132 ) of hal32 was generated by homologous recombination in e. coli. electron microscope pictures revealed the deletion virus could replicate in hzaml cells, which indicates hal32 is not essential for the replication of hasnpv

    通過在大腸桿菌內同源重組構建得到orf132的缺失重組病毒（ hasnpv凸132 ） ，轉染成功后的電鏡切片表明， hasnpv凸132能夠在hzami細胞內正常復制、增殖。
  6. The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination

    為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用，將它們與nifa基因互換載體后共轉化酵母，仍然可以激活三個報告基因的表達，而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。
  7. Compared with nucleic genome, the chloroplast genome is very small and easily to be manipulated genetically, and the foreign gene is site - direct integrated into the chloroplast genome through homologous recombination

    與核轉化相比，葉綠體基因組小，遺傳操作簡單，外源基因是通過同源重組機制定點整合進葉綠體基因組。
  8. Integration through homologous recombination between the 2. 7 kb pks gene and the natural pks gene was confirmed by southern hybridization

    Southern雜交證實2 . 7kbpks基因和天然pks基因之間通過同源重組發生了整合。
  9. 3. the mechanism of dna integration mediated by homologous recombination in chloroplast transformation was tentatively explored. we isolated two chloroplast dna fragments from brassica napus and used them as targeting sequence to integr

    成功地通過h次轉化獲得整合併表達多基因的轉基因煙草，縮短了研究周期，對相關轉基因植物的研究有一定參考價值。
  10. Homologous recombination hr

    同源性重組
  11. Construction of multifunctional genetically engineered pesticides - degrading bacteria by homologous recombination

    同源重組法構建多功能農藥降解基因工程菌研究
  12. This result indicates that u32 glna encodes gs. we also attempted to disrupt and delete u32 reca by homologous recombination but failed

    本論文還嘗試了用同源重組的方法中斷和缺失u32的reca基因，但沒有成功。
  13. The fragment containing mutant gene was subcloned into suicide vector pkng101, named pkh6, pkh 7 and pkh8 respectively. moreover, eaggec 17 - 2 was used to construct gene mutants by homologous recombination

    利用自殺質粒pkng101作為載體，構建了ip氏ip7和if 』 ps基因重組自殺質粒pkll6 、 pkh和嚇。
  14. The dna double strand break ( dsb ) can be rejoined by at least two discrete pathways, i. e., the homologous recombination and the nonhomologous end - joining. at least 10 genes were found involved in nhej

    Rad18是一種單鏈dna結合蛋白，具有許多泛素系統e3所有的典型ring結構，與rad6 （ ubc2 ）形成結合緊密的異二聚體。
  15. But gene targeting was mainly applied to such field as bacterium, yeast and some epiphyte at present, seldom used in higher plants because of the lower frequency of homologous recombination

    但基因打靶技術目前主要應用於細菌，酵母和一些絲狀真菌，在高等植物中應用極少。原因是高等真核生物中同源重組的發生頻率極低。
  16. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
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