homology sequence 中文意思是什麼

homology sequence 解釋
同凋列
  • homology : n. 1. 相應,符合;關系相同。2. 【化學】同系(現象)。3. 【生物學】同源。4. 【數學】透射;同調。
  • sequence : n 1 繼續;接續;連續。2 順序;程序;次第;關系;關聯。3 後果;結果;接著發生的事;後事;後文。4 ...
  1. The homology of deduced bdnf sequences is over 90 % among the three reptiles. but the bdnf sequence of tylototriton taliangensis with those of other three reptiles showed a little lower homology. finally, the homology of bdnf gene among the reptiles and the aves is higher than that among reptiles and in other species

    此外, 4個基因編碼區存在著高度的同源性,除大涼疣螈同源性偏低外,另外3種bdnf基因同源性高達90 ,並且其中的爬行動物bdnf基因與鳥類的同源性高於其它物種。
  2. Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

    以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎,對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。
  3. The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector. their cdna sequences were determined with positive clones or purified pcr product. conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province, their homology is 90. 6 %

    結果:其中一對引物擴增得到一600bp產物;另一對引物擴增得到三條特異性的dna條帶,大小分別為1 . 5kb 、 1 . 3kb和800bp ,將600bp和800bpdna進行克隆及測序,並推導編碼的氨基酸序列。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。
  5. 2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -

    S1基因:其全序列共1614bp (從起始密碼子atg到s前體蛋白裂解位點) ,編碼537個氨基酸,其氨基端有一編碼18個氨基酸的信號肽序列,第12 13位氨基酸殘基構成了信號肽的切割位點, 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。
  6. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染,誘導機體免疫及與宿主細胞相互關系中的作用,本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析,揭示了我國豬瘟病毒流行株之間的遺傳演化關系,為豬瘟病毒的流行病學研究提供依據。
  7. The homology of the other two motifs ( v and vi ), which are also quite conserved in other helicases, were lower than 30 %. the hasn pv helicase protein had considerable amino acid sequence similarity with other baulovirus helicases ( 58 % ), and the highest ( 66 % ) with semnpv and the lowest with xcgv. ( 43 % ). hasnpv helicase was the first helicase reported in single - nucleocapsid nucleopolyhedrovirus there are 5 homologue regions ( hrs ) in hasnpv genome which may play important roles in the viral replication

    同源性比較發現hasnpv解螺旋酶的氨基酸序列與甜菜夜蛾核多角體病毒( spodopteraexiguemnpv , semnpv )的解螺旋酶具有最高的同源性( 66 ) ,與xestiac - nigrum顆粒體病毒( xcgv )解螺旋酶同源性最低( 43 ) , hasnpv解螺旋酶基因是第一個報道的單粒包埋核多角體病毒的解螺旋酶基因。
  8. Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range, phynotype, 16s rrna rflp, 16s rrna sequence, 16s - 23s igs rflp, rapd, rep - pcr, dna - dna hybridization homology. at the same time, the competitive nodulation capacity of rhizobia, effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant

    本研究採用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術系統研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統發育中的地位和相互關系。
  9. Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis

    二、研究論文1 、參照人sry基因hmg - box保守區的序列,設計一對兼并引物, pcr擴增了中華絨螯蟹的sox基因,並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ,其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ,顯示該基因在進化上具高度的保守性。
  10. The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli

    結果表明:運用msha法檢測1型菌毛的檢出率為80 ( 36 45 ) , pcr法的檢出率為95 . 5 ( 43 45 ) , pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強;選擇5株優勢血清型雞源致病性大腸桿菌代表株( o _ ( 89 ) , o _ ( 119 ) , o _ ( 141 ) , o _ ( 127 ) )的1型菌毛pila基因的pcr擴增片段經純化后,分別定向克隆到puc18質粒的多克隆位點,構建了含有目的基因片段的克隆質粒,並轉化到dh5株大腸桿菌載體菌中,篩選獲得陽性克隆菌株。
  11. Homology analysis blast analysis showed that the amino acid sequence of the asf gene cloned from cotton was significantly homologous with adp - ribosylation factor ( asf ) of mammalian, plant and yeast, etc. the amino acid sequence of the gene shows 99 % ( 179 / 180 ), 98 % ( 177 / 180 ), 96 % ( 174 / 180 ) and 92 % ( 168 / 180 ) identity to arabidopsisthalianaarfl, triticum aestivum, solatium tuberosum and bos taurus, respectively

    棉花arf基因的同源性分析應用ncbi進行dna序列的相似性分析,結果表明:棉花arf基因與其它植物、動物和酵母的arf基因具有較高的同源性。棉花arf基因編碼的氨基酸序列與擬南芥,小麥、馬鈴薯、牛的同源性分別達到99 ( 179 / 180 ) 、 98 ( 177 180 ) 。 96 ( 174 180 )楊花腸p核符吞fi曰手谷回伽皿的夭險和92 ( 168ill ) 。
  12. Stilbene synthase genes ( l, 2 ) from vitis venefera were cloned and sequenced. the results of sequence analysis showed the total length of s2 is 1617bp, the homology between s2 and pvin ( reported ) is 99 % ; the total length of si is 1622bp, the homology between si and pvin ( reported ) is 98 %

    Dna序列分析結果表明,來自雷司令的芪合酶基因全長1617bp ,與文獻報道的該基因( pvin )比較,堿基同源率為99 ,來源於粉紅玫瑰的芪合酶基因全長1622bp ,與pvin的序列比較,堿基同源率為98 。
  13. Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b. subtil is chromosomal dna ( from glyb to apre ), and 27 % homology with bacillus sp

    將此重組質粒phchi轉化巨大芽抱桿菌b . megateriumap25 ,得到兩株轉化子,分別命名為p25113一9 , p25113一10 。
  14. Gene sequence analysis showed that the fragment we have obtained has 94 % and 90 % homology with common buckwheat storage protein and legumins, respectively. meanwhile, by analyzing the amino acid sequence, it shares 93 % - 83 % homology with legumins from common buckwheat and sword bean

    採用上述方法獲得的tb22kda核苷酸序列與甜蕎過敏性貯藏蛋白及豆球類蛋白的同源性分別達到94和90 ;氨基酸序列與來自甜蕎中的球蛋白,刀豆蛋白有93 - 83同源性。
  15. Homology analysis showed that pta1 molecule is highly conserved among human, gibbon and monkey, sharing the same sequence about 93 % ~ 95 % at the protein level. pta1 and monoclone antibodies fmu1 - 7 were named as cd226 in 7th workshop and conference on human leucocyte differentiation antigens ( hlda )

    Pta1以及我室制備的一套mabfmu1 - 7在2000年第7屆人類白細胞分化抗原國第四軍醫大學碩士學位論文中文摘要際協作會議中被命名為cd226 。
  16. Its amino acid sequence exhibits significant homology with those of other thrombin - like snake venom enzymes. based on the homology, the catalytic residues and disulfide bridges of gloshedobin were deduced to be as follows : his43, asp88, ser182 and asp176 ; and disulfide bridges, cys7 - 141, cys 28 - 44, cys 76 - 234, cys120 - 188, cys152 - 167 and cys178 - 213

    以同樣的方法,得到長白山白眉蝮蛇( gloydiusussuriensis )毒類凝血酶基因,並比較了它與大連蛇島蝮蛇類凝血酶基因序列的差別,發現兩者的同源性高達98 ,只有三個氨基酸不同: gln ~ ( 66 )
  17. The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned

    從陽性克隆子中提取克隆片段,經序列測定分析,結果表明,克隆片段的cdna大小為816bp ,編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank , embl , ddbj , pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索,結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ;蛋白質氨基酸序列的同源率為90 95 ,表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4
  18. The full orp encodes a 487 - amino acid protein with a calculated molecule weight of 53. 484 kda and an isoelectric point of 6. 75. at the primary sequence level, it shared high homology with clr, so was named clr - like serine protease, clsp. the clsp sequence has been submitted to genbank / embl ( genbank accession number af178985 )

    該蛋白在核酸和氨基酸水平上與人補體組分cl :表現高度同源,故將其命名為補體cl :樣絲氨酸蛋白酶(旦lr一like旦erineprotease , clsp ) ,該基因已經在ge心ank登錄(登錄號為af17s985 ) 。
  19. The sequence of quinolone resistant determing regions ( qrdr ) of gyrase subuint a of salmonella analysis indicated that the gyra gene of strain sll - 3 share 95. 8 % respectively identities with the sequence that griggs reported which showed a high homology between the strains

    對1株耐藥菌株的染色體pcr擴增產物進行了測序,與griggs等在genbank中注冊的沙門氏菌gyra基因qrdr的編碼序列進行比較,同源率為95 . 8 。
  20. Usted homology sequence

    調節同調序列
分享友人