hybrid plasmid 中文意思是什麼
hybrid plasmid
解釋
雜種質粒-
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。 -
The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。 -
In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia
並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。 -
The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination
為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用，將它們與nifa基因互換載體后共轉化酵母，仍然可以激活三個報告基因的表達，而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。 -
The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain
成膜素相關蛋白1 （ phrip1 ）是一個含608個氨基酸的蛋白質，它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制，對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中，根據phrip1的序列設計引物對其進行pcr擴增，得到該基因后將其連接到了pks質粒上，並進一步構建成了誘餌質粒peg202 - phrip1 。 -
Then we cloned the mature ca fragment and the same region of the selected ca fragment from library plasmid to vector pgadt7, but neither did they interact with ga in the assay of two - hybrid system. then we used arabidopsis cam2 isoform as bait to screen the two - hybrid library
之後將成熟ca和文庫中篩到的ca編碼部分的基因分別亞克隆至雙雜交激活域載體pgadt7 ，進行雙雜交檢測它們與g蛋白的相互作用，結果仍為陰性。 -
At first, total 31 transcription factors correlated to cell proliferation, differentiation, physiological stress and apoptosis, were selected ( see in article ) to use in the two - hybrid, and cloned into plasmid pact by using rt - pcr to amplify these transcription factors from fetal tissue cdna or stimulated lymphocyte cdna. as result, only six transcription factors atf3, atf4, e2f6, c - jun, c - fos and p53 were correctly cloned
基於以上的背景信息，本文利用細胞雙雜交技術，對resrin在細胞周期中可能的作用的轉錄因子進行了篩選:一、首先從genebank中篩選了31個與細胞周期密切相關的轉錄因子，設計了特異性引物。從胎兒組織和刺激后的淋巴細胞中克隆了這些轉錄因子並構建於細胞雙雜交系統的pact質粒中。
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