hybridoma 中文意思是什麼

hybridoma 解釋
雜合瘤
  1. The ascites fliud titer of the other hybridoma s2 for further hybridization is 1 : 16000 by blood agglutination. the isotype of 2 - e4 is igg2a, and s2 is iggs

    用於再雜交的另一細胞株為本室保存的分泌抗人a型紅細胞單克隆抗體的雜交瘤細胞株s ； ，其血凝效價為l ： 16000 。
  2. The bispecific antibody tilers of ascites fluid and culture supernatant of 3 - hn are 1 : 16000 and 1 : 64. at the same time the anti - p24 antibody and the anti - human rbc type a antibody in the ascites fluid of hybrid - hybridoma 3 - hn are tested, and their titer is 1 : 800 and 1 : 16000 respectively

    為89士9條j十； ；的腹水型和上清型雙特異性抗體的效價分別為1 ： 16000和1 ： 64 ，同時測定腹水型抗體抗p24抗體活性及抗人a型紅細胞凝集抗體活性，效價分別為1 ： 800和1 ： 16000 。
  3. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  4. 2. monoclonal antibody gl biochem provides the following hybridoma development program

    吉爾生化從2005年2月起開始提供抗體制備服務。
  5. The chromatosome of hybrid - hybridoma 3 - hn are 132 + 15, that of hybridoma 2 - e4 and s2 are 84 + 9 and 89 + 9

    2 e4和s 。在hat液中培養，均於一周內死亡。
  6. 2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody

    選其中一株3 - h _ （ 11 ）做雜交-雜交瘤細胞染色體計數，同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數：制備腹水型和上清型抗體，用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體，即紅細胞固相吸附法測雙特異中文摘要性抗體，紅細胞凝集試驗測單特異性抗人a型紅細胞抗體，間接elisa法測抗p24抗體；用腹水型抗體做耐熱性及耐凍融實驗。
  7. Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but

    通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價，高親和力，較好特異性的抗mt單克隆抗體，最後獲得了5株單克隆細胞株，都能分泌針對mt的特異性抗體，建立了選擇性基團免疫分析法，用制備的抗體測定了人血清中mt的含量，作了mt的抑制標準曲線，其抑制范圍從50pg ? 30ng ，半抑制量為1
  8. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  9. By this procedure, milstein and kohler developed a hybridoma that made a specific antibody.

    米爾斯坦和科勒用此方法培養出一個雜種瘤，后來成為一種特效抗體。
  10. The batch culture of jal1 hybridoma cells

    1雜交瘤細胞的批量培養研究
  11. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
  12. 1975 hybridoma technology deeloped for production of monoclonal antibodies

    1975年，雜交瘤技術被應用於生產單克隆抗體
  13. 1975 hybridoma technology developed for production of monoclonal antibodies

    1975年，雜交瘤技術被應用於生產單克隆抗體
  14. Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained

    方法：基因工程p24抗原免疫小鼠4次，歷時2個月，取脾細胞與骨髓瘤細胞株sp2 0 ，用peg融合， hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞，三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。
  15. Establishment and characterization of two hybridoma cell lines secreting anti - osteosarcoma antibody

    抗人成骨肉瘤雜交瘤細胞系的建立及其特性鑒定
  16. Expression of human papillomavirus type 16 l1 and construction of hybridoma cell strain of human papillomavirus type 16 l1 monoclonal antibody

    1蛋白表達及其單克隆抗體雜交瘤細胞株的建立
  17. Therefore the hybrid - hybridoma can secrete not only bispecific antibody but also anti - human rbc type a monospecific antibody and probably anti - p24 monospecific antibody. the mixed antibodies purified by ammonium sulfate is further purified by p24 affinity chromatography. the unsp

    這樣，分離出雙特異性抗體，並證實雜交雜交瘤除分泌抗p24和人紅細胞的雙特異性抗體外，還分泌抗p24單特異性抗體和抗人a型紅細胞單特異性抗體。
  18. Development of hybridoma cell line excreting monoclonal antibody against total aflatoxins

    抗總黃麴黴毒素單克隆抗體的制備及特性
  19. Methods 1 mr1 hybridoma was cultured in hollow fibre system. culture supernatant was harvested, then precipitated by half - saturated ammonia sulphate. mfm was purified by deae - sephacel ion exchange chromatography. mri concentration was measured by elisa

    硫酸錢半飽和沉澱， oeae陰離子交換層析分離純化抗co40l單克隆抗體mri 。 pbs透析后， 505一隊ge檢測純化抗體的純度， eusa測定抗體濃度。
  20. The isolated at - iii will be used in the screening of positive clones of hybridoma cell lines

    本研究採用常規法進行小鼠免疫和細胞融合。
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