hydantoin 中文意思是什麼
hydantoin
解釋
海因-
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With the procedures, the overall recovery of enzymatic activity reached 20 % and the specific activity for substrate hydantoin was about 4 u / mg protein. the purification factor was about 4. 7 folds with the purity about more than 95 % as estimated by sds - page analysis. d - hydantoinase gene from strain ss - ori was cloned to five different vectors to be five recombinant plasmids and in turn to transfer into five different e. coli strains, respectively
對其中產酶活性最高的一工程菌pexsec一hdt /雲coljblz ] （ de3 ）海因酶的表達條件進行了研究，目的蛋白的表達量約占總菌蛋白的20 % ，其酶活力為0 . 92u / ml ，約是原始菌株的d一海因酶表觀活性的4 . 6倍。 -
Hydantoinase ( ec 3. 5. 2 ) hydrolyzes its substrate 5 ' - monosubstantial hydantoin to enantiomerical n - carbamyl - amino acids and in turn, the resulting product can be chemically or enzymatically converted into the corresponding optically active amino acids
海因酶（ hydantoinase ， ec3 . 5 . 2 ） ，是一類催化海因、 5 』 -單替代海因及其衍生物環酰胺鍵斷裂的酰胺水解酶。 -
In bioconversion of a d - amino acid and its derivative, the substrate, 5 ' - monosubstituted hydantoin is firstly transferred into an intermediate, d - carbamyl amino acid by d - hydantoinase, and in turn into an optically active d - amino acid by either d - carbamoylase or chemical, nano2. nowadays, the bioconversion of d - amino acids has been industrialized by enzymes, so called one - step process
D -型氨基酸及其衍生物的生物轉化，經歷兩個反應歷程，首先是在海因酶的作用下，催化5 -單替代海因形成氨甲酰類氨基酸中間物，後者再在酶或化學的作用下，生成相應的光學活性氨基酸或其衍生物。 -
Hydantoinases which consist of hydantoin hydrolase, n - carbamoylase and sometimes hydantoin racemase can hydrolyze d, l - 5 - substituted hydantoin into corresponding optically active amino acids. therefore, the hydantoinases from micro - organisms are widely applied to the industrial production of natural and unnatural amino acids. microbial hydantoinases are considered as industrial enzymes from the origin of life
乙內酰脲酶是廣泛存在於動物、植物和微生物的一類酶系，包括乙內酰脲水解酶（ hydantoinhydrolase ， hyuh ）和n -氨甲酰基氨基酸水解酶（ n - carbamoylase ， hyuc ） ，有些情況下還包括乙內酰脲消旋酶（ hydantoinracemase ， hyur ） 。 -
The l - n - carbamoylase of arthrobacter bt801, coded by the hyuc gene, is the rate - limiting and the only stereoselective enzyme. hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and, at the same time, activity expression was obtained in e. coli and pichia pastor is gs115 respectively
節桿菌bt801的乙內酰脲水解酶催化乙內酰脲類化合物水解開環， n -氨甲酰基氨基酸水解酶是該菌乙內酰脲酶系中惟一具有立體選擇性的酶，也是整個反應體系的限速酶。 -
Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine. hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801. n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids
節桿菌（ arthrobacter ） bt801是由軍事醫學科學院生物工程研究所保存的l -乙內酰脲酶產酶菌株， puc18 - 169是由本室構建的含有節桿菌bt801的l -乙內酰脲酶完整操縱子序列的亞克隆質粒。 -
To improve the production and activity of the enzyme, the hydantoin hydrolase gene ( hyuh ) was cloned into e. coli m15 and bl21 ( de3 ) by using vector pqe60 and pt221 respectively. two expression plasmids were thus constructed
用pcr技術從質粒puc18 - 169中擴增出l -乙內酰脲水解酶基因（ hyuh ）和n -氨甲酰基氨基酸水解酶基因（ hyuc ） ，分別在大腸桿菌和畢赤酵母中實現了活性表達。
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