ibv 中文意思是什麼

ibv 解釋
傳染性支氣管炎疫苗
  1. 2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -

    S1基因：其全序列共1614bp （從起始密碼子atg到s前體蛋白裂解位點） ，編碼537個氨基酸，其氨基端有一編碼18個氨基酸的信號肽序列，第12 13位氨基酸殘基構成了信號肽的切割位點， 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。
  2. The high specificity of dot - elisa was proved by the specific blocking test and the cross - reaction te st in which the diagnostic diaphragm did n ' t react with the positive serum against ibdv, ibv, eds - 76, pox, ibv, iltv, salmonellosis jc. both the test within batch and the test among batches proved the method or the diagnostic diaphragm was stable. stored at 4c for at least 6 months, the diagnostic diaphragm ' s sensitivity and specificity did n ' t change

    阻斷試驗和交叉試驗表明快診膜具有良好的特異性：不與馬立克、法氏囊、雞痘、雞傳支、雞傳喉、雞減蛋綜合癥、雞傳鼻、雞沙門氏菌等陽性血清反應。批內和批間重復性試驗表明該法重復性良好。診斷膜片4至少可保存6個月，其特異性、靈敏性不變。
  3. There were deletion, insertion or mutation within the ibv isolates, the frontal 150nt showed the high variation

    核苷酸序列前150bp的變異最大，不同毒株間存在插入、缺失及點突變等。
  4. A pair of primer designed according to multi - sequence comparing results of published ibv sequences in genbank was used to amplify the nucleocapsid gene of 5 strains, the amplified rt - pcr product of all 5 strains is 1. 2kb in length

    對3個克隆質粒中的目的基因片段進行序列測定，結果表明該片段含有ibvn基因全序列，其結構基因全長1227bp ，編碼409個氨基酸。
  5. Soybean cotyledonary nodes were transformed with s gene of ibv via a. tumefaciens - med ated transformation. the highest transformation rate of 6. 7 % was detected in the present experiment

    以子葉節為外植體，在抗性篩選培養基上大多白化死亡，有少數生長出芽，轉化頻率最高為6 . 7
  6. The recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease. agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri - parental mating method

    用三親交配法將重組克隆導入根癌農桿菌，獲得了含pbi121重組質粒的eha105農桿菌菌株。
  7. Infectious bronchitis ( ib ) caused by infectious bronchitis virus ( ibv ) is an acute and highly contagious disease in chickens

    傳染性支氣管炎（ ib ）是由傳染性支氣管炎病毒（ ibv ）引起的一種急性高度接觸性雞傳染病。
  8. 90 % of them were positive by pcr. southern blot analysis showed that ibv si gene had been integrated into genomic dna of potato and most transgenic plants have two copies of ibv si gene. northern blot analysis indicated that most transgenic plants could transcribe normally si gene of ibv though the level of transcription was different in the different tansgenic plants

    對所得到的抗性植株進行分子生物學檢測， 90的再生植株pcr檢測為陽性； southernblot檢測證明外源基因已整合到植物基因組內，大多數為二拷貝， northernblot分析表明多數轉基因植株中外源基因能正常轉錄，但轉錄水平不一，也有外源基因發生基因沉默。
  9. The nucleotide sequences and deduced amino acid sequences of the m gene of 8 ibv isolates in china with the published sequences of reference strains in genbank were analyzed and compared, the results showed that the nucleotide sequences homogeneity between them is 87. 1 % ~ 100 %, and the deduced amino acid sequences homogeneity is 88. 1 % ~ 100 %

    將m基因核苷酸序列和推導的氨基酸序列進行比較的結果表明ibv分離株彼此間的核苷酸同源性為87 . 1 - 100 ，其相應的氨基酸序列的同源性為88 . 1 - 100 。
  10. The sequences of the structural protein genes and deduced a mino acid sequence of isolate lx4 were compared. by computer software, complete main structural genes sequence of ibv domestic strain and molecular characteristic genetic - variant analyses, and probably t cell and b cell epitopes of the main structural protein of infectious bronchitis virus were analyzed premently

    通過計算機分析軟體，對我國ibv地方流行毒株lx4的主要結構基因全序列、分子特徵及遺傳變異進行分析比較，並初步分析預測傳染性支氣管炎病毒主要結構蛋白上可能存在的t細胞和b細胞表位。
  11. To explore the feasibility of ib edible vaccine, we have transformed si gene of ibv into potato and researched the immunogenicity of it ' s expression product. the findings are as follows : 1. a pair of primers for ibv si gene were designed and synthesized according to the published nucleotide sequence of ibv si gene and multiclone sites of expression vector pbi121

    為了探索ibv可食性疫苗的可行性，我們進行了轉基因馬鈴薯表達ibv免疫原基因及其表達產物免疫原性的研究，取得以下結果： 1根據周繼勇等報道的ibv - zj971毒株s1基因核苷酸序列和pbi121植物表達載體的多克隆位點，設計併合成引物，以含s1pbs質粒為模板擴增s1基因，將擴增片段定向克隆到pbi121質粒的35s啟動子下游。
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