immobilized antibody 中文意思是什麼
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A novel protein immobilization approach for piezoelectric immunosensors based on the sam of derivative aa has been proposed, which is formed by coupling merca ptoprorionic acid ( mpa ) to alginic acid sodium salt via edc and nhs. ( 1 ) the tr antibody is immobilized via edc and nhs. comparing with the mpa sam method, this new approach shows greater frequency response and high sensitivity
應用edc和nhs使褐藻酸鈉與胱胺偶合形成衍生褐藻酸鈉作新的自組裝材料，用於蛋白固定，應用這一新方法， （ 1 ）實現了轉鐵蛋白抗體分子的固定，並對轉鐵蛋白參考血清進行了測定，該方法可在0 . 08 25 . 7 g / ml范圍內對轉鐵蛋白進行測定。 -
The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwich or competitive immunoreaction
將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。 -
The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwitch or competitive immunoreaction. the surface of immuno - sensor can be renewed by used in a new immunoassay
將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。 -
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
The capture of the labeled antibody ? analyte complex for signal generation can be achieved with the antibody immobilized in a defined area of nitrocellulose membrane as mentioned
對產生信號的標記抗體抗原復合物的捕獲，是用抗體固定在已經提到的硝酸纖維素的特定區域內來達到的。 -
Deviation in the immobilized antibody density is relatively high even with a minute change in experimental conditions, and the amount of detection antibody can be accurately controlled by transferring a metered volume ( 8 )
盡管在實驗條件下只有很小改變，固定抗體的濃度偏差也會相對較高，而檢測抗體的量就能通過移取量好的體積來精確控制。 -
It was proved that the amount of immobilized antibody and the immunoactivity of bound antibodies could be well improved by colloidal au. hrp labeled antibody reacted with antigen, then hrp biocatalyzed dab ( 3, 3 ’ - diaminobenzidine ) in the presence of h _ 2o _ 2, resulting in an insoluble product onto the electrode surface, to achieve an obviously decreased frequency
在h _ 2o _ 2存在下，通過標記在抗人igg抗體上的辣根過氧化物酶（ hrp ）催化底物3 ， 3 』 -聯苯二胺（ dab ） ，反應生成不溶性產物沉積到石英晶振的au電極表面，達到質量放大的目的。 -
A piezoelectric immunosensor was developed by immobilized transferring antiserum on the surface of quartz crystal to especial answer transferring, it can be get good answer to determined in the range of 3. 92 ~ 79mg / l ; the adsorption and desorption of consult serum on the quartz crystal monitored with tr - antibody, determined the rate constant of immuno - reaction was 2. 14xl07 ( mol / l ) ' 1xs ' 1, the association constant of the immuno - reaction ( k1ss ) was 6. 75xl07 ( mol / l ) ~ l
Oz vomg l范圍內的參考血請中的轉鐵蛋白進行了定量測定，獲得很好的響應；利用石英晶體微天平技術在線監測了參考血清在固定了轉鐵蛋白抗體的石英晶振上的吸附與解吸過程，測得了其抗原抗體免疫反應的速率常數為2 14xl0 』 （ mol l ） 』 xs 「 『 ，結合常數為6 -
In determining the optimal concentration of each antibody, we have used a strategy in which the capture antibody is first immobilized at a particular density on the predetermined site of membrane, and the labeled antibody is then varied to retain a maximum signal - to - noise ratio
在測定每個抗體的最佳濃度中，我們用到了這樣一個方案：先將捕獲抗體以特定的濃度固定在膜上預留的部位，然後改變標記抗體濃度得到最大的信號噪音比例。 -
A novel protein immobilization approach based on mercapto - pha sam for piezoelectric immunosensors has been proposed, which is formed by coupling mercaptoprorionic acid ( mpa ) to poly ( allylamine hydrochloride ) ( pha ) via 1 - ethyl - 3 - ( 3 - dimethylamino - propl ) carbodiimide ( edc ) and n - hydroxysuccinimide ( nhs ). after self - assembling a mercapto - pha sam on the gold electrode of the quartz crystal, the goat - anti - igm antibody is immobilized via cross - linking with glutaraldehyde. the detecting linear range of the piezoelectric immunosensor employing mercapto - pha sam is 0. 66 - 26. 4 ug / ml
應用偶聯試劑碳二亞胺（ edc ）和n -羥基琥珀酰亞胺（ nhs ）使聚丙烯胺（ pha ）與巰基丙酸（ mpa ）偶合形成巰基化的聚丙烯胺，應用這一新方法，實現了羊抗人igm抗體分子的固定，並在0 . 66 26 . 4 g / ml范圍內對人血清免疫球蛋白igm進行了測定。 -
Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a. biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction
抗體通過單層蛋白a固定到金電極表面，預先處理好的生物素化抗體和辣根過氧化物酶（ hrp ）標記的親和素形成的網狀復合物作為免疫反應信號的放大探針。 -
The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。 -
The experiment results indicated the immobilized antigen still keep its good reactivity and stability and can use for detecting reactions of antibody - antigen
實驗測試的結果表明，固定后的抗原，保持了良好的反應性和穩定性，可用於抗原抗體反應的測試。 -
After self - assembling a cystamine sam on the gold electrode of the quartz crystal, the a, - at antibody has been immobilized via cross - linking with glutaraldehyde
該方法是一種很有潛力的免疫傳感器抗原抗體分子固定的新方法。
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