immobilized antigen 中文意思是什麼
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The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwich or competitive immunoreaction
將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。 -
The development of renewable amperometric is a possible way to circumvent the problem. here, antigen ( antibody ) is immobilized with graphite ( carbon ) and carrier on a transducer, the analyze is measured through on enzyme catalytic reaction after sandwitch or competitive immunoreaction. the surface of immuno - sensor can be renewed by used in a new immunoassay
將抗原（抗體）與石墨或者碳固定在載體材料中，在一個競爭性的或者夾心式的免疫反應后，將酶標抗原（抗體）鍵合在傳感器表面，通過一個酶催化反應來確定待測抗原（抗體）的濃度。 -
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
It was proved that the amount of immobilized antibody and the immunoactivity of bound antibodies could be well improved by colloidal au. hrp labeled antibody reacted with antigen, then hrp biocatalyzed dab ( 3, 3 ’ - diaminobenzidine ) in the presence of h _ 2o _ 2, resulting in an insoluble product onto the electrode surface, to achieve an obviously decreased frequency
在h _ 2o _ 2存在下，通過標記在抗人igg抗體上的辣根過氧化物酶（ hrp ）催化底物3 ， 3 』 -聯苯二胺（ dab ） ，反應生成不溶性產物沉積到石英晶振的au電極表面，達到質量放大的目的。 -
The newcastle disease antigen ( nd. ag ) was immobilized in a graphite paste matrix
Ab ，一種家禽傳染病） 。把新城疫疾病抗體固定在碳糊電極中。 -
Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a. biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction
抗體通過單層蛋白a固定到金電極表面，預先處理好的生物素化抗體和辣根過氧化物酶（ hrp ）標記的親和素形成的網狀復合物作為免疫反應信號的放大探針。 -
The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。 -
The experiment results indicated the immobilized antigen still keep its good reactivity and stability and can use for detecting reactions of antibody - antigen
實驗測試的結果表明，固定后的抗原，保持了良好的反應性和穩定性，可用於抗原抗體反應的測試。 -
After dissolving inclusion bodies, renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ), the fusion protein trx - scfv of electrophoretic purity was obtained, they still possessed antigen - binding activity
經變性復性，用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體，在一升的發酵液中得到78mg的重組蛋白。
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