immobilized protein 中文意思是什麼

immobilized protein 解釋
固定化蛋白質
  1. A novel protein immobilization approach for piezoelectric immunosensors based on the sam of derivative aa has been proposed, which is formed by coupling merca ptoprorionic acid ( mpa ) to alginic acid sodium salt via edc and nhs. ( 1 ) the tr antibody is immobilized via edc and nhs. comparing with the mpa sam method, this new approach shows greater frequency response and high sensitivity

    應用edc和nhs使褐藻酸鈉與胱胺偶合形成衍生褐藻酸鈉作新的自組裝材料，用於蛋白固定，應用這一新方法， （ 1 ）實現了轉鐵蛋白抗體分子的固定，並對轉鐵蛋白參考血清進行了測定，該方法可在0 . 08 25 . 7 g / ml范圍內對轉鐵蛋白進行測定。
  2. The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in

    實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。
  3. One was based on the sbs sensitive membrane and " gas phase " immunoassay technology. by immobilized anti - tb or anti - trichinosis on the sbs membrane, it can be used to determine m. tuberculosis and trfohiflosjs. the other one was based on the protein a sensitive membrane and " liquid phase " immunoassay technoiogy

    一種是基於sbs膜固定抗體的壓電氣相兔疫技術，我們利用該技術首次對結核病和旋毛蟲病分別進行了快速診斷：另一種是基於蛋白a定向修飾抗體的壓電液相兔疫傳感技術，我們利用該技術首次對結核病進行了診斷。
  4. A novel protein immobilization approach based on mercapto - pha sam for piezoelectric immunosensors has been proposed, which is formed by coupling mercaptoprorionic acid ( mpa ) to poly ( allylamine hydrochloride ) ( pha ) via 1 - ethyl - 3 - ( 3 - dimethylamino - propl ) carbodiimide ( edc ) and n - hydroxysuccinimide ( nhs ). after self - assembling a mercapto - pha sam on the gold electrode of the quartz crystal, the goat - anti - igm antibody is immobilized via cross - linking with glutaraldehyde. the detecting linear range of the piezoelectric immunosensor employing mercapto - pha sam is 0. 66 - 26. 4 ug / ml

    應用偶聯試劑碳二亞胺（ edc ）和n -羥基琥珀酰亞胺（ nhs ）使聚丙烯胺（ pha ）與巰基丙酸（ mpa ）偶合形成巰基化的聚丙烯胺，應用這一新方法，實現了羊抗人igm抗體分子的固定，並在0 . 66 26 . 4 g / ml范圍內對人血清免疫球蛋白igm進行了測定。
  5. Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a. biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction

    抗體通過單層蛋白a固定到金電極表面，預先處理好的生物素化抗體和辣根過氧化物酶（ hrp ）標記的親和素形成的網狀復合物作為免疫反應信號的放大探針。
  6. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
  7. Hydrolysis and mechanism of wastewater contained protein using immobilized protein enzyme

    固定化蛋白酶對廢水中蛋白質的水解效果與機理
  8. After dissolving inclusion bodies, renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ), the fusion protein trx - scfv of electrophoretic purity was obtained, they still possessed antigen - binding activity

    經變性復性，用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體，在一升的發酵液中得到78mg的重組蛋白。
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