inclusion bodies 中文意思是什麼
inclusion bodies
解釋
包含體-
After the expression form analysis, the insoluble recombinant proteins was purified by destraction and abstersion of inclusion bodies. to study the abstersion condition of the inclusion bodies, we adopted ultrasound crushing and freezing - melting methods
採用超聲加洗滌液破碎菌體；離心加凍融分離純化融合蛋白，研究不同的超聲次數和凍融對包涵體洗滌效果的影響。 -
The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times, and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant
用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體，在37條件下，洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體，包涵體在8mol l尿素中的溶解性非常好。 -
Renaturation of thermolabile hemolysin inclusion bodies expressed by vibrio parahaemolyticus
融合表達的副溶血弧菌不耐熱性溶血毒素包涵體復性的研究 -
Then the inclusion bodies were solubilized in 8 mol / l urea - 100 mmol / l p - mercaptoethanol ( - me )
將較為純凈的包涵體溶解於8mo比的尿素溶液中， 4放置過夜。 -
The recombinant protein with the molecular weight of 76 kd was mainly expressed as inclusion bodies in e. coli, which was identified by sds - page
Sds - page證明，重組融合蛋白的分子量約76kd ，並主要以包涵體的形式存在。 -
The hau3r gene was over - expressed when e. coli bl21 ( de3 ) ( phz2055 ) was induced with iptg, but the products existed in the form of inclusion bodies
在大腸桿菌bl21 （ de3 ）中hau3 ~ r基因獲得超量表達，但以無活性的包含體形式存在。 -
Its content was about 9. 8 % among total cell protein by gene genius bio imaging system. the fusion proteins were found largely in an insoluble inclusion bodies. the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105
經工ptg誘導，重組質粒在點co力『 blzi中表達出了c端融合了6xhis的融合蛋白，過量表達的蛋白主要以不溶性蛋白形式存在，其表達量占菌體總蛋白的9 . 8 % 。 -
The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger
膽囊充盈、其內充滿稀薄的膽汁；法氏囊、胸腺萎縮，腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物；腎臟腫大。病理組織學變化方面，早期肝臟、腎臟、腸主要以出血、水腫和壞死為主，且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體，包涵體呈嗜酸性，為大型圓形包涵體或不規則的顆粒狀；法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡，法氏囊濾泡數目增多細胞間有較大空隙。 -
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質 -
The results showed that infected chicken not only had charateristic pathologic changes such as serious degeneration, necrusis, formation of intranuclear inclusion bodies in the hepatic cells, but its immune organs were also seriously injured
結果表明，病雞發生以肝臟嚴重變性、壞死，並在肝細胞核內形成核內色涵體的特徵性病變。 -
Isolate and purify the target protein. prepare 50ml e. coli expressing the interesting proteins and harvest the cells and determinate the solubility of the target protein. completely solubilize inclusion bodies with 8m urea
溶解包涵體，將包涵體溶解物上ni一taslurry ，利用該融合蛋白表達時帶有的his一tag與ni +的親和作用分離、純化表達蛋白。 -
Influences on host plant cell pathology by tumv infection tumv particles were scattered in cytoplasm area of diseased cells separately or in bundles. the pinwheels, scrolls and laminated aggregates, which were the cross sections of cylindrical inclusion bodies, were observed under transmission electron microscope. meanwhile, pathological changes of diseased chloroplasts " morphology and structure took place
Tumv侵染寄主的細胞病理學特徵利用透射電鏡觀察接種寄主細胞的超薄切片，分離自杭州榨菜上的tumv分離物jc - 1在青菜和芥菜的細胞質中病毒粒子分散或成束分佈；細胞質中存在不同形態的柱狀內含體，分別為風輪體、捲筒體、片層聚集體；同時，葉綠體發生了形態和結構上的改變。 -
The purified enzyme had a specific activity of 68. 6 u / mg protein. overproduction of pga was often limited by translocation and / or periplasmic processing steps, subsequently resulted in intracellular accumulation of various types of pga precursors and then formed inclusion bodies in the cytoplasm and / or periplasm
經deae - sepharosecl6b離子交換層析和butyl - sepharosecl4b疏水層析，即可得純度提高20倍、比活為68 . 6u mg的青霉素g酰化酶，兩步純化的總得率達91 。 -
The supernatant fraction and the precipitation fractions were analyzed by western blotfor strain dh5 a / pkkfpga, 5 - 10 % pga precursors formed as inclusion bodies in the cytoplasm while no inclusion bodies formed in the periplasm, this suggested most pga precursors were transported to the periplasm and matured to active pga and indicated that the maturation of pga in strain dh5 / pkkfpga was limited by the translocation step
Western印跡分析表明對于菌株dh5 pkkfpga ， 5 - 10的原前體青黴g素酰化酶在胞內形成了包涵體，說明其成熟的限速步驟在胞內的運輸階段，而菌株dh5 psmlfpga則無明顯包涵體形成，說明菌株dh5 psmlfpga改善了青霉素g酰化酶的合成流，因而其表達能力高於菌株dh5 pkkfpga 。 -
Different hosts " response suggested that tumv - sd1 could infect plants of 10 species in 3 families. tumv - sd1 formed pine - wheel inclusion bodies in plant cells. the coat protein of the tumv - sd1 contains 3 components whose estimated molecular weight are 45kd, 38kd and 14kd respectively
寄主反應特性表明， tumv - sd1 6能侵染3科10種植物， tumv - sd1在寄主細胞內形成風輪狀內含體，外殼蛋白為3組分，分子量分別為45kd 、 38kd和14kd ；提純的病毒粒體為長線條狀。 -
The results show that the optimal conditions of the expression of bl21 ( de3 ) - ptxbl - hng and bl21 ( de3 ) - ptxb1 - m - insuiin are : bl medium, 37, 200rpm for 4. 75h, then 1ptg was added to the medium to 0. 01mmol / l, induce for another 5h. 3. hng fusion protein and m - insulin fusion protein are expressed as inclusion bodies in e. coli
工程菌表達條件的研究山西醫科大學2003屆碩士學位論文表達鑿l株blzi （ de3 ）一ptxbi一hng及bl21 （ de3 ）一ptxbi一介insulin均在bl培養基， 37oc 、 zoorpm搖床培養4小時45分時進入對數生民中後期，此時加入終濃度0 . olmlnol / l的i屍tg開始誘導，再持續培養5小時可達到最人表達量。 -
After washing with reagent, adopt the newest purification technology source30rpc, sds - page and densitometric scan analysis, the result show that expression level is 90 % of total bacterial proteins. after renaturation, ifnr, hgfa, hgfb, hpk5 were purified by akta purifier chromatogram instrument, sepharose fast flow, ssphacrayl series gel, selecting optimize condition. finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies, purification product purity > 98 %
結論:總之，通過對發酵罐中重組工程菌各種培養因素的研究，建立了一種高密度、高表達發酵工藝體系，為重組蛋白的后續純化提供了大量、穩定的原料供應;通過對不同目的蛋白的色譜行為的系統研究，建立了一種高效穩定、快速簡潔、易於放大的包涵體重組蛋白分離純化體系。 -
After sonification, the precipitate was washed repeatedly by ionic and non - ionic detergents to remove membrane proteins as could as possible, and the cleaner inclusion bodies were abtained
其次對包涵體體外復性進行研究。用離子型與非離子型去污劑反復洗滌包涵體，盡可能去除膜蛋白。 -
This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in e. coli, and summarize the most efficient ways to refold recombinant proteins
摘要描述了大腸桿菌異源重組蛋白質的形成、制備、變性和復性，綜述了國內外變性、復性的有效方法。 -
This subject uses the genetic engineering technology and the newest modern biotechnology to study the genetic engineering lower reaches stage, in order to research and establish the optimum, high density fermentation technology pattern and high efficient isolation and purification model of recombinant proteins from inclusion bodies
本課題利用基因工程技術，重點從基因工程下游階段著手研究，利用最新現代生物技術研究並建立經優化的高密度發酵工藝模式及高效分離純化包涵體重組蛋白模式。一
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