incubation temperature 中文意思是什麼

Three different batches of broomcorn millet cultures obtained under uniform conditions in millet processing, initial inocula level, and incubation were found not significantly differing in sporulation capacity. on non - nutritional agar plates, temperature significantly affected sporulaiton

本研究用白碳黑、硅藻土和變色硅膠對新鮮黍米培養物進行處理並在5和10下保存，均導致其快速喪失產抱能力。

The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )

以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。

Abstract : soil incubation experiments were conducted in lab to delineate the effect of soil temperature and soil water content on soil respirations in broad - leaved / korean pine forest ( mountain dark brown forest soil ), dark coniferous forest ( mountain brown coniferous forest soil ) and erman ' s birch forest ( mountain soddy forest soil ) in changbai mountain in september 2001. the soil water content was adjusted to five different levels ( 9, 21, 30, 37 and 43 ) by adding certain amount of water into the soil cylinders, and the soil sample was incubated at 0, 5, 15, 25 and 35 for 24 h the results indicated that in broad - leaved / korean pine forest the soil respiration rate was positively correlated to soil temperature from 0 to 35. soil respiration rate increased with increase of soil water content within the limits of 21 to 37, while it decreased with soil water content when water content was over the range. the result suggested the interactive effects of temperature and water content on soil respiration. there were significant differences in soil respiration among the various forest types. the soil respiration rate was highest in broad - leaved / korean pine forest, middle in erman ' s birch forest and the lowest in dark coniferous forest. the optimal soil temperature and soil water content for soil respiration was 35 and 37 in broad - leaved / korean pine forest, 25 and 21 in dark coniferous forest, and 35 and 37 in erman ' s birch forest. because the forests of broad - leaved / korean pine, dark coniferous and erman ' s birch are distributed at different altitudes, the soil temperature had 4 - 5 variation in different forest types during the same period. thus, the soil respiration rates measured in brown pine mountain soil were lower than those in dark brown forest and those measured in mountain grass forest soil were higher than those in brown pine mountain soil

文摘:為了研究土壤溫度和土壤含水量對闊葉紅松林（山地暗棕壤） 、雲冷杉暗針葉林（山地棕針葉林土壤）和岳樺林（生草森林土）的土壤呼吸的影響，於2001年9月在長白山進行了土壤實驗.利用增加土壤樣柱的含水量，將土壤含水量分為9 ， 、 21 、 30 、 37和43 5個等級，土壤樣品分別在0 、 5 、 15 、 25和35的溫度下保持24小時.闊葉紅松林土壤在0 （ 35范圍內，土壤呼吸速率與溫度呈正相關.在一定的含水量范圍內（ 21 （ 37 ） ，土壤呼吸隨含水量的增加而升高，當含水量超出該范圍，土壤呼吸速率則隨含水量的變化而降低.土壤溫度和水分對土壤呼吸作用存在明顯的交互作用.不同森林類型土壤呼吸作用強弱存在顯著差異，大小順序為闊葉紅松林>岳樺林>雲冷杉暗針葉林.紅松闊葉林土壤呼吸作用的最佳條件是土壤溫度35 ，含水量37 ;雲冷杉暗針葉林下的山地棕色針葉土壤呼吸作用的最佳條件是25 ， 21 ;岳樺林土壤呼吸作用的最佳條件是35 ，含水量37 .但是，由於長白山闊葉紅松林，雲冷杉林和岳樺林處在不同的海拔帶上，同期不同森林類型土壤溫度各不相同，相差4 ~ 5 ，所以野外所測的同期的山地棕色針葉林土呼吸速率應低於暗棕色森林土呼吸速率，山地生草森林土呼吸速率應高於山地棕色針葉林土的呼吸速率.圖2表1參25

Soil incubation experiments were conducted in lab to delineate the effect of soil temperature and soil water content on soil respirations in broad - leaved / korean pine forest ( mountain dark brown forest soil ), dark coniferous forest ( mountain brown coniferous forest soil ) and ermans birch forest ( mountain soddy forest soil ) in changbai mountain in september 2001. the soil water content was adjusted to five different levels ( 9, 21, 30, 37 and 43 ) by adding certain amount of water into the soil cylinders, and the soil sample was incubated at 0, 5, 15, 25 and 35 for 24 h the results indicated that in broad - leaved / korean pine forest the soil respiration rate was positively correlated to soil temperature from 0 to 35. soil respiration rate increased with increase of soil water content within the limits of 21 to 37, while it decreased with soil water content when water content was over the range. the result suggested the interactive effects of temperature and water content on soil respiration. there were significant differences in soil respiration among the various forest types. the soil respiration rate was highest in broad - leaved / korean pine forest, middle in ermans birch forest and the lowest in dark coniferous forest. the optimal soil temperature and soil water content for soil respiration was 35 and 37 in broad - leaved / korean pine forest, 25 and 21 in dark coniferous forest, and 35 and 37 in ermans birch forest. because the forests of broad - leaved / korean pine, dark coniferous and ermans birch are distributed at different altitudes, the soil temperature had 4 - 5 variation in different forest types during the same period. thus, the soil respiration rates measured in brown pine mountain soil were lower than those in dark brown forest and those measured in mountain grass forest soil were higher than those in brown pine mountain soil

為了研究土壤溫度和土壤含水量對闊葉紅松林（山地暗棕壤） 、雲冷杉暗針葉林（山地棕針葉林土壤）和岳樺林（生草森林土）的土壤呼吸的影響，於2001年9月在長白山進行了土壤實驗.利用增加土壤樣柱的含水量，將土壤含水量分為9 ， 、 21 、 30 、 37和43 5個等級，土壤樣品分別在0 、 5 、 15 、 25和35的溫度下保持24小時.闊葉紅松林土壤在0 （ 35范圍內，土壤呼吸速率與溫度呈正相關.在一定的含水量范圍內（ 21 （ 37 ） ，土壤呼吸隨含水量的增加而升高，當含水量超出該范圍，土壤呼吸速率則隨含水量的變化而降低.土壤溫度和水分對土壤呼吸作用存在明顯的交互作用.不同森林類型土壤呼吸作用強弱存在顯著差異，大小順序為闊葉紅松林>岳樺林>雲冷杉暗針葉林.紅松闊葉林土壤呼吸作用的最佳條件是土壤溫度35 ，含水量37 ;雲冷杉暗針葉林下的山地棕色針葉土壤呼吸作用的最佳條件是25 ， 21 ;岳樺林土壤呼吸作用的最佳條件是35 ，含水量37 .但是，由於長白山闊葉紅松林，雲冷杉林和岳樺林處在不同的海拔帶上，同期不同森林類型土壤溫度各不相同，相差4 ~ 5 ，所以野外所測的同期的山地棕色針葉林土呼吸速率應低於暗棕色森林土呼吸速率，山地生草森林土呼吸速率應高於山地棕色針葉林土的呼吸速率.圖2表1參25

Just before use, heat the glucose agar tube in boiling water or flowing steam for 15 min, then cool rapidly to the incubation temperature

使用前在沸騰的水浴中或蒸氣中加熱葡萄糖瓊脂試管15分鐘，然後迅速冷卻至培養溫度。

The recombinant vectors were transformed into e. coli m15 respectively and the expression was induced based on the optimal values of the iptg concentration incubation temperature and induction time determined in the previous section

根據優化確定的iptg誘導濃度、誘導溫度和時間進行誘導表達。 5的積層膠， 15的分離膠，變性聚丙烯酰胺凝膠電泳（ sds - page ）檢查表達情況。

After hrp deposition, majority of its initial activity was lost ; however, the left part which still remains the activity has the high resistance to denaturation environments like high temperature, long incubation time and organic solvent exposure

研究發現，沉積后的hrp活性損失很大，但沉積后仍具有活性的部分酶在高溫、長時間和有機溶劑等環境下表現出了明顯好於游離hrp的催化穩定性。

Fig. 2. effect of temperature on fruit rot development of guava inoculated with botryodiplodia theobromae after 7 days of incubation

圖三、溫度對四種病原所引起的番石榴果腐病病勢進展之影響。

Effect of temperature on incubation and thermal tolerance of the chinese forest frog

溫度對中國林蛙卵孵化和孵出熱耐受性的影響

Clsm, afm and tem testified the deposition of these drugs and the experiments carried out in ( 1 ) different initial incubation concentration, ( 2 ) different temperature, ( 3 ) different nacl concentration and ( 4 ) different ph revealed that the drugs have a relative high deposition efficiency in the high initial incubation concentration, high temperature, high nacl concentration and high ph environment, and relatively stable release properties in the low initial incubation concentration, low temperature, high nacl concentration and low ph environment

在（ 1 ）不同初始濃度、 （ 2 ）不同溫度、 （ 3 ）不同鹽濃度和（ 4 ）不同ph值下對模型藥物rdb和抗癌藥物dnr沉積和釋放的研究發現高初始濃度、高溫、高鹽和高ph值環境下有利於藥物的沉積；而低初始載藥量、低溫、高鹽和低ph值環境下，沉積后的藥物則具有相對平緩的釋放性能。

Fig 1. effect of temperature on mycelial growth of pestalotia psidii ( pp ), colletotrichum gloeosporioides ( cg ), phomopsis sp. ( ps ), phyllosticta sp. ( pa ) and botryodiplodia theobromae ( bt ) after 2 days of incubation on pda

圖一、溫度對於五種番石榴果腐病菌的菌絲在馬鈴薯葡萄糖瓊脂培養基生長的影響。

Incubation lasts anywhere from 24 - 28 days, and seems to be temperature dependent

孵化期為24 ? 28天，具體取決于溫度條件。