indirect staining 中文意思是什麼
indirect staining
解釋
間接染色法-
Using indirect immuno - fluorescein staining method, the localization of sp - 10, tmdc - iii and aeg on human, mouse, rabbit, pig, bull sperm was observed under confocomicroscopy. it was found that antigens sp - 10 and aeg express on acrosome of all kinds sperms, supporting their roles in sperm - egg fusion. it was firstly described that tmdc - iii express mostly on the equator, neck and some part of tail of human sperm, on the acrosome of mouse and bull sperms, on the acrosome and neck of pig sperm, on the head, neck, and middle piece, principal piece of tail of rabbit sperm
利用間接免疫熒光法對aeg 、 sp - 10 、 tmdc -蛋白在人、小鼠、兔子、豬、牛精子上進行了精確定位， aeg 、 sp - 10在頂體部均有表達，確證了它們與精卵融合有關：首次對tmdc -蛋白在人、小鼠、兔子、豬、牛精子進行了精確定位，它主要存在於人精子尾部主段，小鼠和牛精子頂體，豬精子頂體和頸部，兔精子頭部、頸部和尾部的主段及尾段。 -
In this experiment three sperm proeins, sp - 10. aeg. tmdc - iii. that were cloned and expressed by brist u. k., immunized on rats for analysing their immunogenicity and the effects on the fertility. then using indirect immuno - fluorescein staining method, the localization of sp - 10, tmdc - iii and aeg on human, mouse, rabbit, pig, bull sperm was observed under confocomicroscopy. the results as follows : 1. the rats successfully produced antibody after immunization with antigens sp - 10, aeg, tmdc - iii respective
本文用英國葛蘭素公司重組構建表達的三種抗原蛋白sp - io ， aeg ， tmdc -免疫大鼠，通過elisa實驗、免疫熒光組化實驗、交配實驗對它們的免疫原性、在體內的生殖作用、精子表達部位進行了分析，結果如下： 1 -
Wang sp, grayston jt. serotyping of chlamydial trachomatis by indirect fluorescent - antibody staining of inclusions in cell culture with monoclonal antibodies [ j ]. j clin microbiology, 1991, 29 : 1295
余加林，吳仕孝.連接酶鏈反應在沙眼衣原體感染診斷中的應用[ j ] .重慶醫科大學學報， 1996 ， 21 : 409
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