induced sequence 中文意思是什麼

induced sequence 解釋
誘導序列
  • induced : 感生的
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  1. Rapid - sequence induction : anesthesia is most commonly induced by the method of rapid - sequence induction, in which rapid administration of an ultra - short - acting barbiturate ( e. g., thiopental ) is followed by a depolarizing muscle relaxant ( e. g., succinylcholine )

    快速序貫誘導：誘導麻醉最常用的是快速序貫誘導方法，應用此法時先快速給予超短時作用的巴比妥（如硫賁妥鈉） ，接著給去極化的肌肉鬆弛劑（如琥珀膽堿） 。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。
  4. A rapid - sequence induction : anesthesia is most commonly induced by the method of rapid - sequence induction, in which rapid administration of an ultra - short - acting barbiturate ( e. g., thiopental ) is followed by a depolarizing muscle relaxant ( e. g., succinylcholine )

    快速序貫誘導：誘導麻醉最常用的是快速序貫誘導方法，應用此法時先快速給予超短時作用的巴比妥（如硫賁妥鈉） ，接著給去極化的肌肉鬆弛劑（如琥珀膽堿） 。
  5. A cdna library of grass carp ( ctenopharyngodon idellus ) leukocytes stimulated with virus was constructed in order to clone and analyze the immune genes which are induced and expressed by virus infection in the immune system of this fish. initial studies on rapid identification of fish ' s new genes by expressed sequence tag analysis were also undertook in this paper

    為了克隆草魚免疫相關基因，我們構建了草魚白細胞cdna文庫，同時對利用生物信息資源快速鑒定魚類新的功能基因的方法進行了初步探討，為今後深入開展魚類功能基因研究奠定了良好的基礎。
  6. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  7. And many important cis - acting regulatory elements were found in the cloned sequence region by plantcare software analysis. ref promoter was predicted to be most probably induced by light, hot, gibberellin, ethenen or wound

    經plantcare軟體分析發現序列中含有多種調控元件，經預測ref啟動子可能被光、熱、 ga 、乙烯或傷所誘導。
  8. Compared with a reported cmv - cp gene sequence, the homology of nucleotide sequences were 100 %. the sequencing result also demonstrated the recombinant vector pet - 22b - cp has a proper orf encoding 218 amino acids. the recombinant vector was transformed into bl21 ( de3 ) cells. transformants were grown and induced by the addition of isothiopropylgalactoside ( iptg ) to a concentration of imm with continued shaking at 37 ?

    酶切鑒定及序列測定表明，重組表達質粒pet - 22b - cp連接區域符合設計要求，具有正確的開放閱讀框架，插入片段含有218個氨基酸的完整編碼區，其核苷酸序列與報道的cmv - cp基因的同源性為100 。
  9. The sequence and activity characteristics of shuikou reservoir induced earthquake are analyzed with several methods, such as probabilistic evaluation, comprehensive effect parameter e evaluation, two - step comprehensive fuzzy evaluation, the maximum magnitude of historical earthquakes evaluation, etc. the results show that the shuikou m ( subscript l ) 4. 1 earthquake on april 21, 1996 is a main - earthquake, and from now on, the possibility occuring m ( subscript l ) > 3 induced earthquake in shuikou reservoir area is low

    摘要應用概率法、綜合影響參數預測法、兩級模糊評判法、古登堡里克特公式外推預測法等對水口水庫誘發地震序列以及活動特徵進行了研究，認為： 1996年4月21日發生的m （下標l ） 4 . 1地震為水口水庫誘發地震主震，今後庫區發生大於3級以上誘發地震的可能性較小。
  10. Results occurrence rate and mortality of acute renal failure induced by antimicrobials, the urological system drugs and traditional chinese medicines occupied the top 3 places in sequence, and drugs for intravenous administration resulted in the highest rate ( 43. 87 % ) followed by oral drugs ( 42. 29 % )

    結果抗微生物藥物、作用於泌尿系統藥物、中草藥致急性腎衰竭的發生率及死亡率居前3位，靜脈用藥的發生率最高（ 43 . 87 % ） ，其次為口服用藥（ 42 . 29 % ） 。
  11. We validate that the multi - level rough set approximation induced by a sequence of reflexive relations is a special case of cbm - rs

    經過驗證，二元自反關系序列下的多層粗糙集模型是cbm - rs模型的特例。
  12. Secondly, the dissertation proposes a novel blind symbol - timing scheme for ofdm systems based on cyclostationarity feature of received symbols. the proposed schemes also exploits the periodicity of ofdm symbol introduced by cyclic prefix, by applying 2 - dimentional fourier transformation and choosing the appropriate correlation peak value as the symbol start location, the precision of this scheme is higher than previous conventional method. thirdly, the dissertation presents analysis with regard to channel estimation of ofdm systems. several interpolation algorithm in ofdm systems which based on pilot sequence have been analyzed in the first instance, and the influence of the channel noise on interpolation precision has discussed. the theoretic analysis and simulation results show that : the interpolation error induced by the precision of interpolation procedure itself has out weight

    第三，論文在ofdm系統的通道估計方面，先對基於導頻的ofdm通道估計中的多種插值方法進行了分析，討論了噪聲對插值精度的影響，指出插值本身的精度所造成的插值誤差遠大於噪聲所帶來的插值誤差，從而階次更高的插值演算法在實用中並非最優的;並指出插值濾波法比變采樣率演算法對噪聲的影響更為敏感，在信噪比較高時插值濾波演算法比變采樣率演算法更優。
  13. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  14. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
  15. When waves propagate over the ocean surface, a sequence of wave pressure is induced on the mudline or seafloor, which causes the coupling interaction between the deformation of soil skeleton and seepage movements of porous fluid

    海洋表面傳播的波浪在海水-海床的交界面處施加了循環波壓力，在這種循環波壓力作用下，海床內土骨架的變形與孔隙流體的滲流運動相互耦合。
  16. Sequence analysis shows that they share 98. 75 % similarity at the dna, and 98. 67 % at the protein level. ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l. the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss, positive bacterium strain was induced by iptg

    將ns2基因插入到原核表達性質粒pgex - 6p - 1的ecor 、 bamh多克隆位點之間，將重組原核表達質粒pgex - 6p - ns2轉化到bl21 （ de3 ） plyss感受態細胞中，獲得了表達ns2基因的陽性亞克隆重組子，在含amp的lb液體培養基中培養，經iptg誘導表達，用sds - page分析表達產物。
  17. Homology searches using blast with the amino acid sequence revealed that pdipl shares a high degree of homology ( 70 % identity ) with the tnf - induced protein b12

    此外，通過blast檢索發現pdip1蛋白與一個受tnf -誘導的蛋白質b12十分相似，彼此的氨基酸序列高達70是相同的。
  18. M sequence has several constructive methods such as cut - joining, spanning tree, the selected method, the induced method and so on

    M序列的構造方法有剪接法，升級法，選定法以及誘導法等。
  19. Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography

    方法： pcr擴增小鼠pemcdna編碼序列，將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上，轉化大腸桿菌bl21 ，誘導表達gst mpem融合蛋白，通過親和層析，獲得初步純化的產物，以羥胺切割驗證其一級結構。
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