inducible promoter 中文意思是什麼
inducible promoter
解釋
誘導型啟動子-
Histochemical staining in tl transgenic tobaccos showed that sigp1 promoter was not only organ - specific ( especially expressed in leaves and guard cells ), but also highly inducible by multi stress treatment such as drought, aba, high salinity and low temperature. quantitative analysis showed that gus activity in roots was very weak and no great changes were observed after treatment
通過對t1代轉基因苗的組織化學定位表明，該啟動子驅動的gus基因在逆境（如乾旱、 aba 、高鹽和冷）脅迫的葉片中表達，根中不表達，且老葉中的表達量高於幼葉中的表達量，說明其表達受發育階段的調控。 -
Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay
由於vgb基因啟動子不能在枯草桿菌中啟動表達，因此，根據已發表的果聚糖蔗糖酶基因（ sacb ）序列設計引物，從枯草桿菌wb600總dna中擴增出該基因的啟動子片段，然後將其與vgb基因編碼區及終止子序列相連，成功地組建了sacvgb融合基因。 -
To prove that the cloned dna fragment can express tryptopanase, a new plasmid pet28c - tnaa, in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ), a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter, which is inducible by iptg
用iptg誘導表達t7rna聚合酶，以表達質粒上的目的基因。在葡萄糖存在的條件下，用常規方法發酵和誘導（ 37 1mmiptg ） ，發現表達的蛋白質條帶的分子量與理論上計算的分子量一致。但是發酵液中檢測不到吲哚，表明雖然表達了目標蛋白，但表達的蛋白質沒有酶活性。 -
Pks expression plasmids containing strong, temperature - inducible, bacteriophage lambda promoters with or without the escherichia coli phage t7 promoter were constructed and some of them lead to high expression of the expected c
為在大腸桿菌超量表達該2 . 7kbpks基因所編碼的兩個功能結構域，構建了具有不同啟動子組合的pks表達質粒。 -
We have created transgenic tobacco plants in which expression of the atnced3 coding region is under strong constitutive 35s cauliflower mosaic virus ( 35s ) promoter, stress - inducible rd29a promoter or stomotal - specific kst1 promoter by agrobacterium tumefaciens - mediated transformation
構建了組成型35s 、誘導型rd29a 、氣孔特異表達性kst1啟動子驅動atnced _ 3基因表達的真核表達載體，通過農桿菌介導的葉盤法轉化煙草，得到了轉基因植株。 -
The characteristics of various cis - regulatory elements of plant promoters, such as tata - box, transcription start point and upstream elements, and different types of the plant promoter, such as constructive promoter, tissue - specific promoter and inducible promoter, as well as bi - direction promoter, alternative promoter and linked promoter will be described or analyzed in this present paper
並著重從組成型、組織特異型和誘導型啟動子3個方面介紹了其結構特徵、功能，以及它們在植物基因工程中的應用和研究進展，簡述了雙向啟動子、可變啟動子和串聯啟動子的研究情況，提出植物啟動子研究中存在的問題與展望。 -
Base on the successful expression of pks gene by cits857 controlled lambda promoter, an e. coli expression vector phz330, an e. coli and streptomyces bi - functional vector phz1060 were constructed containing the dts857 - pr for inducible expression in both hosts. both vector
根據受cits857調控的p _ r能引導pks基因的成功表達，構建了大腸桿菌表達載體phz330以及在大腸桿菌和鏈黴菌兩種宿主中都能表達外源基因的雙功能表達載體phz1060 。 -
Inserting the anther box into a given area of the chemical - inducible promoter resulted an anther - specific expression of gus upon the induction of chemical inducers, such as bth. 4
在克隆的化學誘導啟動子中適當位點插入花藥盒可以使誘導應答集中於花藥，從而使天然的化學誘導性啟動子變成人工花藥特異性啟動子。
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