inoculate with 中文意思是什麼
inoculate with
解釋
灌輸-
Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle
首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌;制備成菌懸液,紫外燈下照射誘變,紅光下塗抹篩選平板,恆溫培養2 3d ;按透明圈大小進行預初篩,挑選徑圈比大的菌落接斜面,恆溫培養3d ;按一株一瓶的方式進行初篩;從中選取酶活較大的3 5株,按一株2 3瓶的方式進行復篩;挑取酶活大且穩定的1 2株進入下一代誘變篩選。
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