insertion sequences 中文意思是什麼
insertion sequences
解釋
插入順序,插入序列-
The results showed that the f fragment, 728bp in length, could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment, about 4kb in length, is inferred to have repeat sequences around tn5 insertion site, in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica. to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals, the experiment with mice was carried out
結果顯示， f片段長度為728bp ，與現有生物數據庫的blast比較分析，發現該序列僅有局部短於1oobp的區域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性，推測為一新基因； b片段長約4kb ，序列拼接結果推測靠近tn5插入位點部位有重復序列，對b片段tn5遠端的部分序列進行blast比較，發現它與小腸結腸炎耶爾森氏菌的強毒力島有一定的同源性。 -
Some tendency of tn5gusa5 transposition were found that all preferred sites of tn5gusa5 in xcc 8004 genomic dna are in at - rich regions ; target sequences of tn5gusa5 have some features that the probabilities of guanine and cytosine are high respectively at the head and tail base of target sequence ; the level of gene transcription does not influence insertion density of tn5gusa5 significantly
結果表明， tn5gusa5插入位點有一定的規律性： tn5gusa5在xcc8004基因組上傾向于插入低gc含量（ 50左右的區域插入密度最高）區段；插入位點的靶序列有一定的特異性，在靶序列的首位鳥嘌呤出現的幾率高，而在靶序列的末位胞嘧啶出現幾率高； tn5gusa5的插入密度與該區段基因的轉錄水平無明顯關系。 -
Transposable elements, or insertion elements, are dna sequences which can be inserted into many different sites in chromosomes.
轉位因子或插入因子是能夠插入到染色體不同位點的DNA序列。 -
Transposable elements, or insertion elements, are dna sequences which can be inserted into many different sites in chromosomes
轉位因子或插入因子是能夠插入到染色體不同位點的dna序列。 -
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。 -
After amplifying genomic sequences flanked with tn5gusa5 by tail pcr and dna sequencing, then doing blast with genomic sequence of xcc 8004, insertion sites of 10981 mutants were located precisely in xcc 8004 genome
經tail - pcr擴增tn5gusa5旁側序列並測序，經與xcc8004基因組序列比對后獲得精確定位的tn5gusa5插入突變體10981個。 -
Phz621 - phz622, the new gene replacement vector system, had been constructed through the insertion of the 1. 0kb and 1. 4kb flanking sequences of hau3r gene from wild - type s. lividans in the same natural orientation
預期在攜帶有大插入片段的基因置換yac分子進入野生型變鉛青鏈黴菌后， yac分子將通過1 . 4kb或1 -
The gd and ge gene was subcloned into puc18, resulting in pugdge. the fragment from pcdnas. 1 - including hcmv promoter / enhancer, mcs and neomycin resistance gene was inserted into the bamhi and bsteii restrication sites of pugdge, resulting in the universal transfer vector pgd - m - ge. the universal transfer vector pgd - m - ge has deleted the gi gene and 363bp in the 5 ' end of the ge orf of prv. there were 11 restrication sites for insertion of the foreign gene. the upstream and downstream flanking sequences were up to 1. 25kb and 1. 42kb. it will be useful for developing the recombinant prv expressing foreign gene ( s )
將gd 、 ge基因連接于質粒puc18獲得pugdge ，缺失質粒pugdge的bamh和bste位點間391bp的片段。在此缺失位置插入來自質粒pcdna3 . 1 -的一偽狂犬病病毒gd 、 ge 、 tk基因的克隆與通用轉移載體的構建段含hcmv啟動子。多克隆位點和neo報告基因的片段，構建了通用轉移載體ppd m pe 。 -
Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1
通過ecori酶切突變株基因組，得到完整的tn5 （含有在大腸桿菌中起始復制的oriv ）及其側翼的序列片段，該片段自連后轉化大腸桿菌，以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明， 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部，本實驗證明它們和042bm耐鹽相關，命名為rst - 0x1和rst - 0x2基因。
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