ion exchange chromatography 中文意思是什麼

ion exchange chromatography 解釋
離子交換層析法
  • ion : n. 【物理學】離子。 positive [negative] ion正[負]離子。
  • exchange : vt 1 （以某物與另一物）交換，調換 （for） 2 互換，交流，交易。3 兌換。 vi 1 兌換 （for） 2 交換；...
  • chromatography : n. 【化學】層析，色層（分離）法。
  1. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。
  2. This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column

    使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法，從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。
  3. A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc. hwtx - v has 35 amino acid residues, with the molecular mass 4111. 4da. the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh, which consists of 6 cys, formed three pairs of disulfide bridges

    本文報道從虎紋捕鳥蛛（ selenocoimahuwena ）粗毒中，結合陽離子交換和反相高效液相色譜分離純化出一種昆蟲毒素，命名為虎紋捕鳥蛛毒素- （ huwentoxin - ， hwtx - ） ；其天然突變體（ themutantofhuwentoxin ， mhwtx - ）也同時純化出來。
  4. Using i - dopa as a specific substrate, phenoloxidase ( po ) from penaeus chinensis hemolymph was purified by gel - filtration and ion - exchange chromatography, and characterized in terms of its molecular weight and enzymatic properties in this study

    本文以中國對蝦（ penaeuschinensis ）的血淋巴為材料，利用凝膠過濾和離子交換等方法，對酚氧化酶（ phenoloxidase ）進行了分離純化和生物化學性質研究。
  5. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  6. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  7. Sodium tripolyphosphate for industrial use - separation by ion exchange chromatography and determination of the different phosphate forms

    工業三聚磷酸鈉離子交換柱色譜法分離測定不同形式的磷酸鹽
  8. Determination of sulfur amino acids in feedstuffs - ion exchange chromatography

    飼料中含硫氨基酸測定方法-離子交換色譜法
  9. Ion - exchange chromatography a type of chromatography in which the compounds are separated according to their acidity

    離子交換色譜法：是一種根據混合物酸度將其分離的色譜技術。
  10. To reveal the reason of above phenomenon, we purified the eluant by poros 20hq ion - exchange chromatography and got five proteins. one of them ( hq - s ) was proved to can be proteolytic

    為弄清這一現象的產生原因，將嗜硫色譜洗脫峰通過poros20hq離子交換色譜進一步分離，獲得5個分子量非常接近的組分。
  11. Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity

    通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法，從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。
  12. High performance ion exchange chromatography was applied in studying qualitatively and quantitatively of bacteria, which was shown as follows : firstly, physio - biochemical characteristics of bacteria was investigated by ion exchange chromatography. for the first time spores and nutrient of bacillus pumilus had been separated successfully by chromatography. chromatographial behaviors of bacteria at different cultivating environment and different growth phase were also studied

    本文利用高效液相離子交換色譜系統研究細菌學，探討了該方法在細菌定性、定量方面的應用，主要包括三個方面：首先，利用離子交換色譜系統表徵細菌生理、生態方面的變化，首次成功地在色譜上區分了短小芽孢桿菌的芽孢及營養體；考察了不同的培養環境對細菌色譜行為的影響及不同生長階段的細菌的色譜行為。
  13. Polyethylene glycol - accompanied ion - exchange chromatography to purify recombinant hepatitis b virus surface antigen

    聚乙二醇伴隨式離子交換層析分離重組乙肝病毒表面抗原
  14. But the whole level of serum titer in combined vaccine group was higher than others. igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose

    對分離到的血清用飽和硫酸銨鹽析法提取igg ，並用deae纖維素離子交換層析法對提取的igg進行純化。
  15. In this paper, one interactive protein was isolated and purified further by ion - exchange chromatography, whose molecular weight was determined by sds - page to be 24kda under non - reduced conditions and 25kda under reduced conditions

    最後，分離純化作用蛋白中分子量為24kda的蛋白，發現其存在鏈內二硫鍵，二硫鍵斷裂后的分子量為25kda 。
  16. The bioactive strain ' s fermentation product was isolated and purified primarily using methods of solvent extraction, acid - alkali extraction, ab - 8 macropore absorption chromatography, 1 x 007 positive ion exchange chromatography, the result showed the purification product has prominent bioactivity inhibit staphalococcus aureus

    中國熱帶農業科學院、華南熱帶農業大學2003屆碩士研究生採用有機劑抽提法、酸堿抽提法、大孔吸附樹脂柱層析和陽離子交換樹脂柱層析等方法對hsl 306菌株發酵產物進行了初步分離純化。
  17. One is a combination of ion exchange chromatography on q sepharose ff, hydrophobic interaction chromatography on phenyl hp, gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q. the other is a combination of ion exchange chromatography on q sepharose ff, two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially

    上清液中重組蝗蛇毒類激血酶的純化是通過設計的不同分離純化方法組合進行的，並對每一種組合進行了活力與純度及口收率的評價，結果表明兩種組合方式都具有一定的實用性和可操作性。
  18. Although ion exchange chromatography is used popularly in determination of chloride in environmental, biological samples, it is rarely used in the determination of chloride in plant because it is easily affected by the ph and the matter coexisted

    摘要離子色譜分析雖已廣泛應用於食品、農業、環境保護及醫療衛生等日常監測和研究工作，但由於其對待測組份的共存干擾物質及酸堿度要求較苛刻，因此目前用於穀物類樣品分析的方法還較少。
  19. Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease. serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography. the t profile, t stability profile, ph profile, substrate specificity and inhibitor were tested

    該酶分子n -端的氨基酸序列為avidtgveashpef ，通過n -端氨基酸序列設計簡並引物，提取被毛孢owvt - 1的總rna ， rt - pcr克隆了該酶的成熟肽編碼基因（ 1000bp ） ，序列分析表明，絲氨酸含量高達12以上。
  20. The purified badh, which became a single band in sds - page, has been obtained by a combination of ammonium sulfate fractionation, ion exchange chromatography and gel filtration chromatography

    遼寧堿蓬甜菜堿醛脫氫酶分子量為129kd ，單個亞基的分子量為64 . 5kd ，表明其為同型二聚體。
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