isolated protein 中文意思是什麼
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The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。 -
A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101. the complete sequence analysis of the plasmid show that it consists of 3176 bps, which contains four putative orfs. orf1 encodes a putative protein and is highly similar to repa which functions in replication
從屎腸球菌df101菌株中分離到隱秘的小質粒pefr ，全序列分析顯示質粒pefr由3176bp組成，編碼四個推定的orf ， orf1編碼的一個推定的蛋白和復制有關的repa有很高的相似性。 -
Whole mount in situ hybridization showed that amphihmgb transcripts were present ubiquitously in the early embryos from zygote to neurula, but they decreased gradually in mesoderm and endoderm and neural tube with development, and became restricted to epidermal cells in the hatched larva. it appear s that hmgb plays a role in the mitosis and differentiation of embryonic cells. another amphioxus cdna, amphiubf80, encoding ubiquitin / ribosomal fused protein s27a, was isolated from the gut cdna library of b. belcheri tsingtauense
S004cdna序列編碼一個156個氨基酸殘基組成的蛋白質，與人的泛素蛋白ubf80具有較高的氨基酸序列同源性（ 145 / 156同源） ，且該蛋白具有泛素蛋白ubf80所共有的兩個結構域，即由72個氨基酸組成的泛素介導的蛋白降解結構域和由48個氨基酸組成的核糖體蛋白s27a結構域。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
We isolated the badh cdna ( acbadh ) from the halophyte atriplex centralasiatica iljin using rt - pcr and the deduced protein sequences showed high similarity with other plants
我們以鹽生植物中亞濱藜（ atriplexcentralasiaticailjin ）為材料，用pt - pcr方法克隆了badhcdna 。 -
For uncovering the effects of reversible phospharylation on the structure and function of psii reaction centre, we purified a protein phosphatase associated membrane from the thykaloid membrane of ipomoea aquatica chloroplasts. in our experiments we studied the enzymology and spect rum characters of the purified phosphatase. in our lab, one kind of protein phosphatase associated thylakoid membrane of pomoea aquatica has been isolated
迄今人們對類囊體蛋白磷酸酯酶的研究較少，為了研究可逆磷酸化對psii反應中心結構與功能的影響，本文以蕹菜為材料，從葉綠體類囊體膜中分離純化到一種膜結合蛋白磷酸酯酶，進行了酶學性質和光譜性質的研究。 -
Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated
解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。 -
In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。 -
The targeted disruption of mouse rbp - j revealed that homozygous null mutants die before 10. 5 days postcoitum ( dpc ) with various abnormalities, including growth retardation and defects in the central nervous system and somites. in order to assess the function of rbp - j, a yeast two - hybrid screening using rbp - j as a bait protein was performed and a nuclear matrix protein, mint was isolated
Sdpc胚胎和人的hela細胞cdna文庫中篩選到一個可以與rbp斗相互作用的新蛋白ram7 ，后發laram7與mmt （ msxz inter肌tingnucleartargetprotein ）為同一蛋白。 -
With different wheat flour as the material and isolated soybean protein as addition agent to manufacture frozen dumplings, the effect of soybean protein on the frozen dumpling quality was studied
摘要以陜168 ， 3 - 2 ，普冰143 ， 31 - 1 - 12和981共5個不同品種（系）的小麥粉為原料，以大豆蛋白為添加劑製作冷凍水餃，研究大豆分離蛋白對冷凍水餃質量的影響。 -
The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75
從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。 -
Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced
首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。 -
Trichosanthin ( tcs ) is a single - chain ribosome inactivating protein. tcs belongs to a sort of metalloprotein, and ca2 + locates in its active center. it is isolated from trichosanthes kirilowii tubers of cucurbitaceae and is the active component of chinese drug tian hua fen
天花粉蛋白是從葫蘆科植物栝樓塊根中分離得到的一種單鏈核糖體失活蛋白，是中藥天花粉的有效成分，具有重要的理論研究和實際應用價值。 -
It was found that prophenoloxidase ( propo ), isolated as a monomeric protein, had a molecular weight of 87. 5 kda, and a 77 kda phenoloxidase molecule was often contained in preparations. the 87. 5 kda propo had a very low enzymatic activity on 0. 02 mol / l l - dopa, whereas the 77 kda po had a very high enzymatic activity on it
實驗發現，酚氧化酶原（ prophenoloxidase ）的分子量為87 . 5kda左右，在實驗操作過程中極易發生降解或自身降解，變成有活性的大小約77kda的酚氧化酶， 1 sds可使該酶原全部被激活而成為有活性的酚氧化酶。 -
The new protein ( tsk - 2 ) was isolated and purified to homogeneity by tsk g3000sw chromatography from the elutriant of thiophilic adsorption, which was incubated in the condition of 40 for 2 hours
將嗜硫色譜的洗脫峰在40水浴中保溫2小時后，再通過tskg3000sw分離得到新的蛋白組分（ tsk - 2 ） 。 -
In this research, the wheat, rice and tobacco with salt treatment were used as experimental materials to study the molecular mechanism of stress - tolerance in plant and make the relative gene engineering research. the main results are as following : 1. a novel ca2 + channel gene, named as tatpc1, was isolated from wheat, encoding a two - pore voltage - gated channel protein
本研究在總結當前植物鹽脅迫應答分子生物學研究進展的基礎上，以農作物小麥、水稻、煙草作為主要實驗材料，開展了植物耐逆分子機制和相關基因工程研究，主要結果為： 1從小麥中克隆到一個新的ca ~ （ 2 + ）通道蛋白基因， tatpc1 ，它編碼的是一種細胞膜上兩孔電壓門控式通道蛋白。 -
A novel chitosan ( cts ) - binding protein was isolated from the non - heading chinese cabbage leaves by chitosan affinity chromatography and partially characterized
說明cts在調控氨同化關健酶gdh和gs活性時，可能有ca 』 +信號系統的參與。 -
Our main products include all kinds of soy sauce powde, hvp ( hydrolyzed vegetable protein ) ， hap ( hydrolyzed animal protein ), isp ( isolated soy protein powder ), vinegar powder, broad bean catsup powder, sweet catsup powder, preserved beancurd powder, meat powder, mustard powder, pumpkin powder, etc
我們的產品主要有醬油粉、水解植物蛋白粉、水解動物蛋白粉、大豆分離蛋白粉、醋粉、豆瓣醬粉、甜面醬粉、腐乳粉、肉粉、芥末粉、南瓜粉等。 -
Isolated wheat protein bodies appear as evenly stained particles.
游離的小麥蛋白質看起來好象均勻染色的顆粒。 -
Isolated soy protein
離析大豆蛋白
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