isolated reaction 中文意思是什麼
isolated reaction
解釋
隔離反應-
Biotransformation in organic solvent is an attractived field in nowadays. compared with isolated enzyme whole cell is not used broadly as biocatalyst. in this research the cells of baker ' s yeast is adopted to mediated a model reaction in organic solvent, in which geraniol is converted to citronellol reductively
有機相生物轉化是當前生物技術中一個具有理論研究意義和應用價值的領域，目前該領域的研究大多集中在利用分離的酶進行生物催化，利用微生物完整細胞進行的研究比較少。 -
For uncovering the effects of reversible phospharylation on the structure and function of psii reaction centre, we purified a protein phosphatase associated membrane from the thykaloid membrane of ipomoea aquatica chloroplasts. in our experiments we studied the enzymology and spect rum characters of the purified phosphatase. in our lab, one kind of protein phosphatase associated thylakoid membrane of pomoea aquatica has been isolated
迄今人們對類囊體蛋白磷酸酯酶的研究較少，為了研究可逆磷酸化對psii反應中心結構與功能的影響，本文以蕹菜為材料，從葉綠體類囊體膜中分離純化到一種膜結合蛋白磷酸酯酶，進行了酶學性質和光譜性質的研究。 -
G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。 -
In this paper, 45 e. coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e. coli by animal test. type 1 pili of 45 strains isolated was detected by msha. the pila gene of 45 avian pathogenic e. coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank. results showed that pcr was more sensitive, faster and more characteristic than msha to detect type 1 pili
本研究將從四川規模化雞場分離鑒定、經1日齡雛雞致病性試驗得到的雞源致病性大腸桿菌45株，採用d -甘露糖敏感血凝試驗（ msha ）檢測1型菌毛，根據genbank中公布的人源大腸桿菌1型菌毛pila基因序列設計一對引物用pcr擴增雞源致病性大腸桿菌1型菌毛pila基因。 -
A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf
根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。 -
In order to understand the epidemiological behaviors of this pathogen, we studied strains e. coli 0157 isolated from patients and dung beetles in 1999 and 2000 in xuzhou city, jiangsu province, by methods of pcr, antimicrobial susceptibility, biochemical reaction and pfge ( pulse - field gel electrophoresis ) of chromosomal dna digested by restriction enzyme xbal
為了探索我國大腸桿菌o157 ： h7感染的流行病學特點，我們使用了聚合酶鏈反應、生化反應、抗生素敏感實驗、脈沖電場凝膠電泳等分子生物學方法，對我國1999 - 2000年徐州地區分離到的部分大腸桿菌o157 ： h7進行了分析。 -
The proviral genome cdna clone of snv strain was digested, and the fragments was cloned into the puc18 vector, sequenced, finally we got the sequence of snv genome. the two pairs of primers were designed and synthesized according to the snv strain env sequence. the env genes of the rev isolates isolated from china were amplified by polymerase chain reaction ( pcr )
根據網狀內皮增生癥病毒snv株env基因的序列，設計並且合成了兩對引物，利用該引物，以中國地方分離株sd9901 、 ha9901前病毒基因組cdna為模板，通過pcr技術，成功的從國內分離得兩株rev毒株中擴增出env基因，並將之克隆進pucm - t載體中測序。 -
The ghr cdna was isolated from southern catfish ( silurus meridionalis ) by reverse transcription polymerase chain reaction followed by rapid amplification of cdna ends
用rt - per加race的方法從南方大口鯰中克隆出其生長激素受體（ ghr ） 。 -
In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。 -
The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
應用逆轉錄-聚合酶鏈式反應（ rt - pcr ）技術克隆得到編碼草魚生長激素（ cgh ）的基因cdna ，並定向克隆到puc18載體上。 -
In this study, the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced. the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues
研究採用反轉錄?聚合酶鏈式反應（ rt ? pcr ）技術對兩株分別從柳州（ gxlz ）和南寧（ gxnn ）分離的廣西流行豬瘟病毒（ classicalswinefevervirus ， csfv ）進行e _ 2全基因的擴增、克隆和測序。擴增片段長度為1090bp ，編碼364個氨基酸殘基。 -
Dendritic cells were isolated from peripheral blood and cultured, then the expression of surface markers, hladr cd86 cd80 cd40 cd14 and cd11c were detected before and after treatment by flow cytometry, and the function of dcs was also evaluated by mixed lymphocyte reaction determination once before treatment and once after treatment
分離培養外周血dc ，流式細胞儀測定dc表面hladr cd86 cd80 cd40 cd14 cd11c的表達，混合淋巴細胞反應測定兩組dc的功能，治療前後各檢測1次。
分享友人