jurkat 中文意思是什麼

jurkat 解釋
尤爾卡特
  1. It is interesting that pma plus calcium ionophore a23187 can inhibit pma - induced pta1 expression, and this effect ca n ' t be reversed by calcmeurin inhibiter fk506. pta1 mabs can inhibit ctl activation and differentiation in mixed lymphocyte culture system when added at the beginning of the culture but can induce platelet activation and aggregation in the fc dependent manner. in 1997, pta1 cdna was cloned from cdna library of tpa activated jurkat cells, which belongs to immunoglobulin superfamily ( igsf ) with two v - like domains of extracelluar region of pta1

    Il - 2 、 tnf - 、 pma可以使t細胞pta1表達上調， tgf -可以下調pta1的表達，而pma加上鈣離子載體a23187可以顯著抑制pma的上調作用，且這種抑制作用不被calcineurin抑制劑fk506所逆轉， 1997年burns教授從pma活化的jurkat細胞cdna文庫中克隆了pta1cdna全長，證實pta1是一個新分子，屬于免疫球蛋白超家族，胞膜外區有兩個v樣結構域。
  2. After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells, the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope, indirect immunofluorescence and flow cytometer analysis. prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed

    建系細胞培養上清與肝癌細胞作用后，經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。
  3. Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y. after confirmed by sequncing. pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod

    方法：本文採用rt ? ? pcr的方法擴增jurkatt淋巴瘤細胞特異性重排的tcr可變區基因片段，克隆到真表達載體pcdna _ 3中，經序列測定無誤后，堿裂解法大量提取質粒，制備dna疫苗。
  4. Results : we obtained the recombinant plasmid containing idiotypic tcryv1 fragment of the jurkat cell line

    結果：本文構建了jurkat淋巳媲細胞系獨特型tcrvvidna重組載體pcdna 。 1cry 。
  5. After screening by g418, jurkat cell lines were established to express scfvs stably

    穩定轉染jurkat細胞，經g4篩選，建立了穩定表達scfv融合蛋白的細胞系。
  6. Jurkat cell lines were established to express scfv fusion proteins stably. the products of stably transfect cells and their activities were analysed

    穩定轉染jurkat細胞，建立穩定表達scfv融合蛋白的細胞系，並分析其表達產物的活性。
  7. ( 2 ) after transiently transfected in cos - 7 cells, secreting expression were detect. jurkat cell lines were construct to express scfvs stably. the activities of the products were confirmed

    穩定轉染jurkat細胞，經篩選獲得穩定表達scfv融合蛋白的細胞系，並證實表達產物具有與hbsag特異性結合的功能。
  8. Effect of cholesterol defeciency on the activity of protein tyrosine kinases in jurkat cells

    細胞蛋白酪氨酸激酶活性的影響
  9. Our results showed that, in the activated mouse spleen t lymphocytes and self - proliferative jurkat cells, actin bound to brgl, nfl / ctf and rna polymerase ii during gene transcription. whereas, in unactivated mouse spleen t lymthocytes, no binding could be found

    結果表明，在活化的小鼠脾t淋巴細胞和自主增殖的jurkat細胞中，肌動蛋白可以與mf復合物、轉錄因於nfi ctf和rna聚合酶11結合。
  10. The results are shown as follows : 1, western blot analysis confirmed that baf complex and nf1 / ctf exist in the nuclei of self - proliferative jurkat cells

    主要結果如下： 1 、通過免疫印記實驗證實，在自主增殖的jurkat細胞核中，存在baf復合物和轉錄因子nf1 ctf 。
  11. Regulation of soluble trail and membrane trail in jurkat cells by pma

    腎綜合征出血熱患者體內可溶型和膜型
  12. Conclusion : we successfully obtained the lymphoma specific tcr y vi recombinant plasmid of the jurkat cell line. the expressive gene fragment of tcr y vi was 333bp long. analyses of the tcryvl fragment showed that it contaied three antigen epitopes

    在pcdna3 tcry組及pcdna3 tcry一il一2組小鼠中用rt ? ? pcril均檢測到了重組質粒在肌肉中的mrna表達。結論： 1 、成功構建ju水扯細胞系r 。
  13. 2, immuno - co - precipitation showed that in the activated mouse spleen t lymphocytes and self - proliferative jurkat cells, brg1, nf1 / ctf and rna polymerase ii were closely bound together, and this was not found in un - activated cells

    2 、免疫共沉澱實驗證明，在活化的小鼠脾t淋巴細胞和自主增殖的jurkat細胞中， brg1 、 nf1 ctf和rna聚合酶是緊密結合在一起的，在沒有活化的小鼠脾t淋巴細胞中這三種蛋白質沒有免疫共沉澱現象。
  14. In this dissertation, jurkat cells, hela cells and mouse spleen t lymphocytes were chosen as experimental materials to answer the question. with the aid of various techniques such as elisa, immuno - co - precipitation, indirect immunofluoresent co - localization, double - labeling immunoelectron microscopy and so on, the relationships of baf complex with nf1 / ctf and rna polymerase ii were careful observed and analyzed

    本文以jurkat細胞、 hela細胞和小鼠脾t淋巴細胞為研究材料，通過酶聯免疫吸附實驗（ elisa ） 、免疫共沉澱、免疫熒光共定位和免疫電鏡雙標記等實驗，觀察和分析了baf復合物與轉錄因子nf1 ctf和rna聚合酶在基因轉錄活動中的相互聯系。
  15. 34 we further investigate the apoptosis related gene. using different concentration of il - 16 treat jurkat cells in 1640 without serum, the expression level of bid and c - myc increase together with the increase il - 16 concentration. these suggest that bid and c - myc may take a part in the apoptosis induced by high concentration of il - 16

    結果表明，在無血清培養jurkat細胞24hr后，隨著rhil ? 16濃度的增加， jurkat細胞的凋亡程度也增加，與之相應， bid和c一myc的表達水平也增加，這表示bdc m廠可能參與il叫6誘導的扣水ct細胞凋亡的調節。
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