klac 中文意思是什麼
klac
解釋
克拉奇-
Cloning and expression in e. coli of lactase. specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis. klac gene was amplified by pcr and subsequently cloned
乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母（ kluyveromyceslactis ）菌株k538的基因組dna為模板，設計引物，利用pcr獲得乳糖酶基因klac ，經dna測序驗證，得到克隆t1549 。 -
Klac gene was ligated to the pet - 30a ( + ) vector for expression. then the recombinant plasmid petlac4 was transformed into e. coli bl21. the positive transformants were induced at different temperature for three hours by iptg with the final concentration of lmm. sds - page analysis and lactase activity assay showed that klac gene was expressed in e. coli, and that the expression level at 28 was much higher than that at 37
表達產物的sds - page分析和酶活性測定表明， klac基因在大腸桿菌中獲得活性表達，其中低溫條件28下的表達水平明顯高於37 ， 28誘導的細胞經超聲波破碎所測酶活力為0 . 475u / ml ， 37僅為0 . 134u / ml 。
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