laser flow cytometry 中文意思是什麼
laser flow cytometry
解釋
激光流血球計數-
We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast
本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。 -
In this work, effects of sfamnpv on mitochondrial and er in sl - 1 cells were studied. the main results were as follows : effect of sfamnpv ( moi = 6 ) on the function of mitochondrial in sl - 1 cells was investigated by mtt assay and membrane potential was assayed by both flow cytometry and confocal laser scanning microscope
完成的工作包括以下幾個方面： （ 1 ）細胞內線粒體的變化通過mtt法研究病毒感染對細胞線粒體功能的影響，同時用流式細胞儀和共聚焦激光顯微鏡研究線粒體膜電位（ m ）的變化， western檢測2種凋亡調控蛋白cytoc和bcl - 2蛋白的變化。 -
Under the flow cytometry assay, the effect of sfamnpv on mitochondrial membrane potential ( m ) was investigated by rh - 123 staining of cells. the rh - 123 fluorescent intensity in sl - 1 cells was reduced in time - dependent manner. the results of confocal laser scanning microscope confirmed that sfamnpv was able to reduce awm
Westernblotting檢測線粒體cytoc和bcl - 2含量的的變化，結果顯示抗凋亡蛋白bcl - 2在病毒的感染過程中含量是逐漸降低的，即病毒的感染導致bcl - 2基因表達的下降。
分享友人